Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity

Abstract Psychological stress has been linked to the development and exacerbation of overactive bladder symptoms, as well as afferent sensitisation in other organ systems. Therefore, we aimed to investigate the effects of water avoidance stress on bladder afferent nerve activity in response to bladd...

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Autores principales:	Kylie A. Mills, Eliza G. West, Donna J. Sellers, Russ Chess-Williams, Catherine McDermott
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b0cacc02675478ea0c4931856fb83a7
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spelling	oai:doaj.org-article:5b0cacc02675478ea0c4931856fb83a72021-12-02T16:37:37ZPsychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity10.1038/s41598-021-97053-52045-2322https://doaj.org/article/5b0cacc02675478ea0c4931856fb83a72021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97053-5https://doaj.org/toc/2045-2322Abstract Psychological stress has been linked to the development and exacerbation of overactive bladder symptoms, as well as afferent sensitisation in other organ systems. Therefore, we aimed to investigate the effects of water avoidance stress on bladder afferent nerve activity in response to bladder filling and pharmaceutical stimulation with carbachol and ATP in mice. Adult female C57BL/6J mice were exposed to either water avoidance stress (WAS) for 1 h/day for 10 days or normal housing conditions. Voiding behaviour was measured before starting and 24-h after final stress exposure and then animals were euthanised to measure afferent nerve activity in association with bladder compliance, spontaneous phasic activity, contractile responses, as well as release of urothelial mediators. WAS caused increased urinary frequency without affecting urine production. The afferent nerve activity at low bladder pressures (4–7 mmHg), relevant to normal physiological filling, was significantly increased after stress. Both low and high threshold nerves demonstrated enhanced activity at physiological bladder pressures. Urothelial ATP and acetylcholine release and bladder compliance were unaffected by stress as was the detrusor response to ATP (1 mM) and carbachol (1 µM). WAS caused enhanced activity of individual afferent nerve fibres in response bladder distension. The enhanced activity was seen in both low and high threshold nerves suggesting that stressed animals may experience enhanced bladder filling sensations at lower bladder volumes as well as increased pain sensations, both potentially contributing to the increased urinary frequency seen after stress.Kylie A. MillsEliza G. WestDonna J. SellersRuss Chess-WilliamsCatherine McDermottNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kylie A. Mills Eliza G. West Donna J. Sellers Russ Chess-Williams Catherine McDermott Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
description	Abstract Psychological stress has been linked to the development and exacerbation of overactive bladder symptoms, as well as afferent sensitisation in other organ systems. Therefore, we aimed to investigate the effects of water avoidance stress on bladder afferent nerve activity in response to bladder filling and pharmaceutical stimulation with carbachol and ATP in mice. Adult female C57BL/6J mice were exposed to either water avoidance stress (WAS) for 1 h/day for 10 days or normal housing conditions. Voiding behaviour was measured before starting and 24-h after final stress exposure and then animals were euthanised to measure afferent nerve activity in association with bladder compliance, spontaneous phasic activity, contractile responses, as well as release of urothelial mediators. WAS caused increased urinary frequency without affecting urine production. The afferent nerve activity at low bladder pressures (4–7 mmHg), relevant to normal physiological filling, was significantly increased after stress. Both low and high threshold nerves demonstrated enhanced activity at physiological bladder pressures. Urothelial ATP and acetylcholine release and bladder compliance were unaffected by stress as was the detrusor response to ATP (1 mM) and carbachol (1 µM). WAS caused enhanced activity of individual afferent nerve fibres in response bladder distension. The enhanced activity was seen in both low and high threshold nerves suggesting that stressed animals may experience enhanced bladder filling sensations at lower bladder volumes as well as increased pain sensations, both potentially contributing to the increased urinary frequency seen after stress.
format	article
author	Kylie A. Mills Eliza G. West Donna J. Sellers Russ Chess-Williams Catherine McDermott
author_facet	Kylie A. Mills Eliza G. West Donna J. Sellers Russ Chess-Williams Catherine McDermott
author_sort	Kylie A. Mills
title	Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_short	Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_full	Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_fullStr	Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_full_unstemmed	Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
title_sort	psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5b0cacc02675478ea0c4931856fb83a7
work_keys_str_mv	AT kylieamills psychologicalstressinducedbladderoveractivityinfemalemiceisassociatedwithenhancedafferentnerveactivity AT elizagwest psychologicalstressinducedbladderoveractivityinfemalemiceisassociatedwithenhancedafferentnerveactivity AT donnajsellers psychologicalstressinducedbladderoveractivityinfemalemiceisassociatedwithenhancedafferentnerveactivity AT russchesswilliams psychologicalstressinducedbladderoveractivityinfemalemiceisassociatedwithenhancedafferentnerveactivity AT catherinemcdermott psychologicalstressinducedbladderoveractivityinfemalemiceisassociatedwithenhancedafferentnerveactivity
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Psychological stress induced bladder overactivity in female mice is associated with enhanced afferent nerve activity

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