Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs

Basavaraj Binjawadagi,1,2 Varun Dwivedi,1 Cordelia Manickam,1,2 Kang Ouyang,1 Yun Wu,3 Ly James Lee,3 Jordi B Torrelles,4 Gourapura J Renukaradhya1,21Food Animal Health Research Program, Ohio Agricultural Research and Development Center, 2Department of Veterinary Preventive Medicine, Ohio State Uni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Binjawadagi B, Dwivedi V, Manickam C, Ouyang K, Wu Y, Lee LJ, Torrelles JB, Renukaradhya GJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5b17d0654b3341a8bb7803da6174afa6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5b17d0654b3341a8bb7803da6174afa6
record_format	dspace
spelling	oai:doaj.org-article:5b17d0654b3341a8bb7803da6174afa62021-12-02T04:21:18ZAdjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs1178-2013https://doaj.org/article/5b17d0654b3341a8bb7803da6174afa62014-01-01T00:00:00Zhttp://www.dovepress.com/adjuvanted-polylactic-co-glycolic-acid-nanoparticle-entrapped-inactiva-a15630https://doaj.org/toc/1178-2013 Basavaraj Binjawadagi,1,2 Varun Dwivedi,1 Cordelia Manickam,1,2 Kang Ouyang,1 Yun Wu,3 Ly James Lee,3 Jordi B Torrelles,4 Gourapura J Renukaradhya1,21Food Animal Health Research Program, Ohio Agricultural Research and Development Center, 2Department of Veterinary Preventive Medicine, Ohio State University, Wooster, OH, USA; 3NanoScale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, 4Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH, USAAbstract: Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is an economically devastating disease, causing daily losses of approximately $3 million to the US pork industry. Current vaccines have failed to completely prevent PRRS outbreaks. Recently, we have shown that poly(lactic-co-glycolic) acid (PLGA) nanoparticle-entrapped inactivated PRRSV vaccine (NP-KAg) induces a cross-protective immune response in pigs. To further improve its cross-protective efficacy, the NP-KAg vaccine formulation was slightly modified, and pigs were coadministered the vaccine twice intranasally with a potent adjuvant: Mycobacterium tuberculosis whole-cell lysate. In vaccinated virulent heterologous PRRSV-challenged pigs, the immune correlates in the blood were as follows: 1) enhanced PRRSV-specific antibody response with enhanced avidity of both immunoglobulin (Ig)-G and IgA isotypes, associated with augmented virus-neutralizing antibody titers; 2) comparable and increased levels of virus-specific IgG1 and IgG2 antibody subtypes and production of high levels of both T-helper (Th)-1 and Th2 cytokines, indicative of a balanced Th1–Th2 response; 3) suppressed immunosuppressive cytokine response; 4) increased frequency of interferon-γ+ lymphocyte subsets and expanded population of antigen-presenting cells; and most importantly 5) complete clearance of detectable replicating challenged heterologous PRRSV and close to threefold reduction in viral ribonucleic acid load detected in the blood. In conclusion, intranasal delivery of adjuvanted NP-KAg vaccine formulation to growing pigs elicited a broadly cross-protective immune response, showing the potential of this innovative vaccination strategy to prevent PRRS outbreaks in pigs. A similar approach to control other respiratory diseases in food animals and humans appears to be feasible.Keywords: porcine reproductive and respiratory syndrome, mucosal vaccine, nanoparticles, cross-protection, pigsBinjawadagi BDwivedi VManickam COuyang KWu YLee LJTorrelles JBRenukaradhya GJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 679-694 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Binjawadagi B Dwivedi V Manickam C Ouyang K Wu Y Lee LJ Torrelles JB Renukaradhya GJ Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs
description	Basavaraj Binjawadagi,1,2 Varun Dwivedi,1 Cordelia Manickam,1,2 Kang Ouyang,1 Yun Wu,3 Ly James Lee,3 Jordi B Torrelles,4 Gourapura J Renukaradhya1,21Food Animal Health Research Program, Ohio Agricultural Research and Development Center, 2Department of Veterinary Preventive Medicine, Ohio State University, Wooster, OH, USA; 3NanoScale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, 4Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH, USAAbstract: Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is an economically devastating disease, causing daily losses of approximately $3 million to the US pork industry. Current vaccines have failed to completely prevent PRRS outbreaks. Recently, we have shown that poly(lactic-co-glycolic) acid (PLGA) nanoparticle-entrapped inactivated PRRSV vaccine (NP-KAg) induces a cross-protective immune response in pigs. To further improve its cross-protective efficacy, the NP-KAg vaccine formulation was slightly modified, and pigs were coadministered the vaccine twice intranasally with a potent adjuvant: Mycobacterium tuberculosis whole-cell lysate. In vaccinated virulent heterologous PRRSV-challenged pigs, the immune correlates in the blood were as follows: 1) enhanced PRRSV-specific antibody response with enhanced avidity of both immunoglobulin (Ig)-G and IgA isotypes, associated with augmented virus-neutralizing antibody titers; 2) comparable and increased levels of virus-specific IgG1 and IgG2 antibody subtypes and production of high levels of both T-helper (Th)-1 and Th2 cytokines, indicative of a balanced Th1–Th2 response; 3) suppressed immunosuppressive cytokine response; 4) increased frequency of interferon-γ+ lymphocyte subsets and expanded population of antigen-presenting cells; and most importantly 5) complete clearance of detectable replicating challenged heterologous PRRSV and close to threefold reduction in viral ribonucleic acid load detected in the blood. In conclusion, intranasal delivery of adjuvanted NP-KAg vaccine formulation to growing pigs elicited a broadly cross-protective immune response, showing the potential of this innovative vaccination strategy to prevent PRRS outbreaks in pigs. A similar approach to control other respiratory diseases in food animals and humans appears to be feasible.Keywords: porcine reproductive and respiratory syndrome, mucosal vaccine, nanoparticles, cross-protection, pigs
format	article
author	Binjawadagi B Dwivedi V Manickam C Ouyang K Wu Y Lee LJ Torrelles JB Renukaradhya GJ
author_facet	Binjawadagi B Dwivedi V Manickam C Ouyang K Wu Y Lee LJ Torrelles JB Renukaradhya GJ
author_sort	Binjawadagi B
title	Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs
title_short	Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs
title_full	Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs
title_fullStr	Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs
title_full_unstemmed	Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs
title_sort	adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/5b17d0654b3341a8bb7803da6174afa6
work_keys_str_mv	AT binjawadagib adjuvantedpolylacticcoglycolicacidnanoparticleentrappedinactivatedporcinereproductiveandrespiratorysyndromevirusvaccineelicitscrossprotectiveimmuneresponseinpigs AT dwivediv adjuvantedpolylacticcoglycolicacidnanoparticleentrappedinactivatedporcinereproductiveandrespiratorysyndromevirusvaccineelicitscrossprotectiveimmuneresponseinpigs AT manickamc adjuvantedpolylacticcoglycolicacidnanoparticleentrappedinactivatedporcinereproductiveandrespiratorysyndromevirusvaccineelicitscrossprotectiveimmuneresponseinpigs AT ouyangk adjuvantedpolylacticcoglycolicacidnanoparticleentrappedinactivatedporcinereproductiveandrespiratorysyndromevirusvaccineelicitscrossprotectiveimmuneresponseinpigs AT wuy adjuvantedpolylacticcoglycolicacidnanoparticleentrappedinactivatedporcinereproductiveandrespiratorysyndromevirusvaccineelicitscrossprotectiveimmuneresponseinpigs AT leelj adjuvantedpolylacticcoglycolicacidnanoparticleentrappedinactivatedporcinereproductiveandrespiratorysyndromevirusvaccineelicitscrossprotectiveimmuneresponseinpigs AT torrellesjb adjuvantedpolylacticcoglycolicacidnanoparticleentrappedinactivatedporcinereproductiveandrespiratorysyndromevirusvaccineelicitscrossprotectiveimmuneresponseinpigs AT renukaradhyagj adjuvantedpolylacticcoglycolicacidnanoparticleentrappedinactivatedporcinereproductiveandrespiratorysyndromevirusvaccineelicitscrossprotectiveimmuneresponseinpigs
_version_	1718401312532463616

Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs

Ejemplares similares