A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea

Abstract Chickpea (Cicer arietinum L.) is the third most important food legume crop. Seed size is the most economically important trait for chickpea. To understand the genetic regulation of seed size in chickpea, the present study established a three-way association of CT repeat length variation of...

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Autores principales:	Vikas Dwivedi, Swarup Kumar Parida, Debasis Chattopadhyay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b313e687677463d97ec8f4c441bb175
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spelling	oai:doaj.org-article:5b313e687677463d97ec8f4c441bb1752021-12-02T12:30:25ZA repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea10.1038/s41598-017-05332-x2045-2322https://doaj.org/article/5b313e687677463d97ec8f4c441bb1752017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05332-xhttps://doaj.org/toc/2045-2322Abstract Chickpea (Cicer arietinum L.) is the third most important food legume crop. Seed size is the most economically important trait for chickpea. To understand the genetic regulation of seed size in chickpea, the present study established a three-way association of CT repeat length variation of a simple sequence repeat (SSR) in myo-inositol monophosphatase gene (CaIMP) with seed weight and phytic acid content by large scale validation and genotyping in a set of genetically diverse germplasm accessions and two reciprocal intra-specific mapping populations. Germplasms and mapping individuals with CT repeat-length expansion in the 5′ untranslated region of CaIMP exhibited a pronounced increase in CaIMP protein level, enzymatic activity, seed-phytate content and seed weight. A chickpea transient expression system demonstrated this repeat-length variation influenced the translation of CaIMP mRNA, apparently by facilitating translation initiation. Our analyses proposed that the SSR marker derived from 5′ UTR of a CaIMP gene is a promising candidate for selection of seed size/weight for agronomic trait improvement of chickpea.Vikas DwivediSwarup Kumar ParidaDebasis ChattopadhyayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vikas Dwivedi Swarup Kumar Parida Debasis Chattopadhyay A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea
description	Abstract Chickpea (Cicer arietinum L.) is the third most important food legume crop. Seed size is the most economically important trait for chickpea. To understand the genetic regulation of seed size in chickpea, the present study established a three-way association of CT repeat length variation of a simple sequence repeat (SSR) in myo-inositol monophosphatase gene (CaIMP) with seed weight and phytic acid content by large scale validation and genotyping in a set of genetically diverse germplasm accessions and two reciprocal intra-specific mapping populations. Germplasms and mapping individuals with CT repeat-length expansion in the 5′ untranslated region of CaIMP exhibited a pronounced increase in CaIMP protein level, enzymatic activity, seed-phytate content and seed weight. A chickpea transient expression system demonstrated this repeat-length variation influenced the translation of CaIMP mRNA, apparently by facilitating translation initiation. Our analyses proposed that the SSR marker derived from 5′ UTR of a CaIMP gene is a promising candidate for selection of seed size/weight for agronomic trait improvement of chickpea.
format	article
author	Vikas Dwivedi Swarup Kumar Parida Debasis Chattopadhyay
author_facet	Vikas Dwivedi Swarup Kumar Parida Debasis Chattopadhyay
author_sort	Vikas Dwivedi
title	A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea
title_short	A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea
title_full	A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea
title_fullStr	A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea
title_full_unstemmed	A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea
title_sort	repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5b313e687677463d97ec8f4c441bb175
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A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea

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