Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.

Tocopherols are a class of four natural compounds that can provide nutrition and function as antioxidant in both plants and animals. Maize kernels have low α-tocopherol content, the compound with the highest vitamin E activity, thus, raising the risk of vitamin E deficiency in human populations rely...

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Autores principales: Qing Li, Xiaohong Yang, Shutu Xu, Ye Cai, Dalong Zhang, Yingjia Han, Lin Li, Zuxin Zhang, Shibin Gao, Jiansheng Li, Jianbing Yan
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Publicado: Public Library of Science (PLoS) 2012
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spelling oai:doaj.org-article:5b31a7b2f52a496ab2706d487866590b2021-11-18T07:18:41ZGenome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.1932-620310.1371/journal.pone.0036807https://doaj.org/article/5b31a7b2f52a496ab2706d487866590b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22615816/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Tocopherols are a class of four natural compounds that can provide nutrition and function as antioxidant in both plants and animals. Maize kernels have low α-tocopherol content, the compound with the highest vitamin E activity, thus, raising the risk of vitamin E deficiency in human populations relying on maize as their primary vitamin E source. In this study, two insertion/deletions (InDels) within a gene encoding γ-tocopherol methyltransferase, Zea mays VTE4 (ZmVTE4), and a single nucleotide polymorphism (SNP) located ~85 kb upstream of ZmVTE4 were identified to be significantly associated with α-tocopherol levels in maize kernels by conducting an association study with a panel of ~500 diverse inbred lines. Linkage analysis in three populations that segregated at either one of these three polymorphisms but not at the other two suggested that the three polymorphisms could affect α-tocopherol content independently. Furthermore, we found that haplotypes of the two InDels could explain ∼33% of α-tocopherol variation in the association panel, suggesting ZmVTE4 is a major gene involved in natural phenotypic variation of α-tocopherol. One of the two InDels is located within the promoter region and associates with ZmVTE4 transcript level. This information can not only help in understanding the underlying mechanism of natural tocopherol variations in maize kernels, but also provide valuable markers for marker-assisted breeding of α-tocopherol content in maize kernels, which will then facilitate the improvement of maize as a better source of daily vitamin E nutrition.Qing LiXiaohong YangShutu XuYe CaiDalong ZhangYingjia HanLin LiZuxin ZhangShibin GaoJiansheng LiJianbing YanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36807 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Qing Li
Xiaohong Yang
Shutu Xu
Ye Cai
Dalong Zhang
Yingjia Han
Lin Li
Zuxin Zhang
Shibin Gao
Jiansheng Li
Jianbing Yan
Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
description Tocopherols are a class of four natural compounds that can provide nutrition and function as antioxidant in both plants and animals. Maize kernels have low α-tocopherol content, the compound with the highest vitamin E activity, thus, raising the risk of vitamin E deficiency in human populations relying on maize as their primary vitamin E source. In this study, two insertion/deletions (InDels) within a gene encoding γ-tocopherol methyltransferase, Zea mays VTE4 (ZmVTE4), and a single nucleotide polymorphism (SNP) located ~85 kb upstream of ZmVTE4 were identified to be significantly associated with α-tocopherol levels in maize kernels by conducting an association study with a panel of ~500 diverse inbred lines. Linkage analysis in three populations that segregated at either one of these three polymorphisms but not at the other two suggested that the three polymorphisms could affect α-tocopherol content independently. Furthermore, we found that haplotypes of the two InDels could explain ∼33% of α-tocopherol variation in the association panel, suggesting ZmVTE4 is a major gene involved in natural phenotypic variation of α-tocopherol. One of the two InDels is located within the promoter region and associates with ZmVTE4 transcript level. This information can not only help in understanding the underlying mechanism of natural tocopherol variations in maize kernels, but also provide valuable markers for marker-assisted breeding of α-tocopherol content in maize kernels, which will then facilitate the improvement of maize as a better source of daily vitamin E nutrition.
format article
author Qing Li
Xiaohong Yang
Shutu Xu
Ye Cai
Dalong Zhang
Yingjia Han
Lin Li
Zuxin Zhang
Shibin Gao
Jiansheng Li
Jianbing Yan
author_facet Qing Li
Xiaohong Yang
Shutu Xu
Ye Cai
Dalong Zhang
Yingjia Han
Lin Li
Zuxin Zhang
Shibin Gao
Jiansheng Li
Jianbing Yan
author_sort Qing Li
title Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
title_short Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
title_full Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
title_fullStr Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
title_full_unstemmed Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
title_sort genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/5b31a7b2f52a496ab2706d487866590b
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