Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.

Diaporthe species are fungal plant pathogens of many important crops. Seed decay is one of the most important diseases on soybean. It is caused by various species of the genus Diaporthe and responsible for significant economic damage. In central Europe the four species D. longicolla, D. caulivora, D...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Behnoush Hosseini, Ralf T Voegele, Tobias I Link
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b32f78784ca40dda7f4fe942a46c491
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5b32f78784ca40dda7f4fe942a46c491
record_format	dspace
spelling	oai:doaj.org-article:5b32f78784ca40dda7f4fe942a46c4912021-12-02T20:14:40ZEstablishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.1932-620310.1371/journal.pone.0257225https://doaj.org/article/5b32f78784ca40dda7f4fe942a46c4912021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257225https://doaj.org/toc/1932-6203Diaporthe species are fungal plant pathogens of many important crops. Seed decay is one of the most important diseases on soybean. It is caused by various species of the genus Diaporthe and responsible for significant economic damage. In central Europe the four species D. longicolla, D. caulivora, D. eres, and D. novem are considered the principal species of Diaporthe on soybean. Fast and accurate detection of these pathogens is of utmost importance. In this study four species-specific TaqMan primer-probe sets that can be combined into a quadruplex assay were designed based on TEF sequences. The specificity and efficiency of the primer-probe sets were tested using PCR products and genomic DNA from pure cultures of the four Diaporthe species and other soybean fungal pathogens. Our results indicate that the primer-probe sets DPCL, DPCC, DPCE, and DPCN allow discrimination of D. longicolla, D. caulivora, D. eres, and D. novem, respectively, and can be used to detect and quantify these four Diaporthe species in parallel using quadruplex real-time PCR. In addition, the quadruplex real-time PCR assay was evaluated on different plant materials including healthy and infected soybean seeds or seed lots, soybean stems, and soybean leaves. This assay is a rapid and effective method to detect and quantify Diaporthe species from samples relevant for disease control.Behnoush HosseiniRalf T VoegeleTobias I LinkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257225 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Behnoush Hosseini Ralf T Voegele Tobias I Link Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.
description	Diaporthe species are fungal plant pathogens of many important crops. Seed decay is one of the most important diseases on soybean. It is caused by various species of the genus Diaporthe and responsible for significant economic damage. In central Europe the four species D. longicolla, D. caulivora, D. eres, and D. novem are considered the principal species of Diaporthe on soybean. Fast and accurate detection of these pathogens is of utmost importance. In this study four species-specific TaqMan primer-probe sets that can be combined into a quadruplex assay were designed based on TEF sequences. The specificity and efficiency of the primer-probe sets were tested using PCR products and genomic DNA from pure cultures of the four Diaporthe species and other soybean fungal pathogens. Our results indicate that the primer-probe sets DPCL, DPCC, DPCE, and DPCN allow discrimination of D. longicolla, D. caulivora, D. eres, and D. novem, respectively, and can be used to detect and quantify these four Diaporthe species in parallel using quadruplex real-time PCR. In addition, the quadruplex real-time PCR assay was evaluated on different plant materials including healthy and infected soybean seeds or seed lots, soybean stems, and soybean leaves. This assay is a rapid and effective method to detect and quantify Diaporthe species from samples relevant for disease control.
format	article
author	Behnoush Hosseini Ralf T Voegele Tobias I Link
author_facet	Behnoush Hosseini Ralf T Voegele Tobias I Link
author_sort	Behnoush Hosseini
title	Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.
title_short	Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.
title_full	Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.
title_fullStr	Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.
title_full_unstemmed	Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.
title_sort	establishment of a quadruplex real-time pcr assay to distinguish the fungal pathogens diaporthe longicolla, d. caulivora, d. eres, and d. novem on soybean.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5b32f78784ca40dda7f4fe942a46c491
work_keys_str_mv	AT behnoushhosseini establishmentofaquadruplexrealtimepcrassaytodistinguishthefungalpathogensdiaporthelongicolladcaulivoraderesanddnovemonsoybean AT ralftvoegele establishmentofaquadruplexrealtimepcrassaytodistinguishthefungalpathogensdiaporthelongicolladcaulivoraderesanddnovemonsoybean AT tobiasilink establishmentofaquadruplexrealtimepcrassaytodistinguishthefungalpathogensdiaporthelongicolladcaulivoraderesanddnovemonsoybean
_version_	1718374645581742080

Establishment of a quadruplex real-time PCR assay to distinguish the fungal pathogens Diaporthe longicolla, D. caulivora, D. eres, and D. novem on soybean.

Ejemplares similares