Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen

Ning Xia,1 Dehua Deng,1,2 Yiru Wang,1 Chao Fang,3 Su-Juan Li1 1Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People’s Republic of China; 2College of Chemistry and Chemica...

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Autores principales:	Xia N, Deng D, Wang Y, Fang C, Li S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Colorimetric assay gold nanoparticles prostate-specific antigen ascorbic acid Cu2+ ion Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5b3503e60e26475b853a118c938081b6
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spelling	oai:doaj.org-article:5b3503e60e26475b853a118c938081b62021-12-02T01:47:03ZGold nanoparticle-based colorimetric method for the detection of prostate-specific antigen1178-2013https://doaj.org/article/5b3503e60e26475b853a118c938081b62018-04-01T00:00:00Zhttps://www.dovepress.com/gold-nanoparticle-based-colorimetric-method-for-the-detection-of-prost-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ning Xia,1 Dehua Deng,1,2 Yiru Wang,1 Chao Fang,3 Su-Juan Li1 1Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People’s Republic of China; 2College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan, People’s Republic of China; 3School of Chemical Engineering, Xiamen University Malaysia, Sepang, Selangor, Malaysia Background: Prostate-specific antigen (PSA), a serine protease, is a biomarker for preoperative diagnosis and screening of prostate cancer and monitoring of its posttreatment. Methods: In this work, we reported a colorimetric method for clinical detection of PSA using gold nanoparticles (AuNPs) as the reporters. The method is based on ascorbic acid (AA)-induced in situ formation of AuNPs and Cu2+-catalyzed oxidation of AA. Specifically, HAuCl4 can be reduced into AuNPs by AA; Cu2+ ion can catalyze the oxidation of AA by O2 to inhibit the formation of AuNPs. In the presence of the PSA-specific peptide (DAHSSKLQLAPP)-modified gold-coated magnetic microbeads (MMBs; denoted as DAHSSKLQLAPP-MMBs), complexation of Cu2+ by the MMBs through the DAH–Cu2+ interaction depressed the catalyzed oxidation of AA and thus allowed for the formation of red AuNPs. However, once the peptide immobilized on the MMB surface was cleaved by PSA, the DAHSSKLQ segment would be released. The resultant LAPP fragment remaining on the MMB surface could not sequestrate Cu2+ to depress its catalytic activity toward AA oxidation. Consequently, no or less AuNPs were generated. Results: The linear range for PSA detection was found to be 0~0.8 ng/mL with a detection limit of 0.02 ng/mL. Because of the separation of cleavage step and measurement step, the interference of matrix components in biological samples was avoided. Conclusion: The high extinction coefficient of AuNPs facilitates the colorimetric analysis of PSA in serum samples. This work is helpful for designing of other protease biosensors by matching specific peptide substrates. Keywords: colorimetric assay, gold nanoparticles, prostate-specific antigen, ascorbic acid, Cu2+ ionXia NDeng DWang YFang CLi SDove Medical PressarticleColorimetric assaygold nanoparticlesprostate-specific antigenascorbic acidCu2+ ionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 2521-2530 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Colorimetric assay gold nanoparticles prostate-specific antigen ascorbic acid Cu2+ ion Medicine (General) R5-920
spellingShingle	Colorimetric assay gold nanoparticles prostate-specific antigen ascorbic acid Cu2+ ion Medicine (General) R5-920 Xia N Deng D Wang Y Fang C Li S Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen
description	Ning Xia,1 Dehua Deng,1,2 Yiru Wang,1 Chao Fang,3 Su-Juan Li1 1Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People’s Republic of China; 2College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan, People’s Republic of China; 3School of Chemical Engineering, Xiamen University Malaysia, Sepang, Selangor, Malaysia Background: Prostate-specific antigen (PSA), a serine protease, is a biomarker for preoperative diagnosis and screening of prostate cancer and monitoring of its posttreatment. Methods: In this work, we reported a colorimetric method for clinical detection of PSA using gold nanoparticles (AuNPs) as the reporters. The method is based on ascorbic acid (AA)-induced in situ formation of AuNPs and Cu2+-catalyzed oxidation of AA. Specifically, HAuCl4 can be reduced into AuNPs by AA; Cu2+ ion can catalyze the oxidation of AA by O2 to inhibit the formation of AuNPs. In the presence of the PSA-specific peptide (DAHSSKLQLAPP)-modified gold-coated magnetic microbeads (MMBs; denoted as DAHSSKLQLAPP-MMBs), complexation of Cu2+ by the MMBs through the DAH–Cu2+ interaction depressed the catalyzed oxidation of AA and thus allowed for the formation of red AuNPs. However, once the peptide immobilized on the MMB surface was cleaved by PSA, the DAHSSKLQ segment would be released. The resultant LAPP fragment remaining on the MMB surface could not sequestrate Cu2+ to depress its catalytic activity toward AA oxidation. Consequently, no or less AuNPs were generated. Results: The linear range for PSA detection was found to be 0~0.8 ng/mL with a detection limit of 0.02 ng/mL. Because of the separation of cleavage step and measurement step, the interference of matrix components in biological samples was avoided. Conclusion: The high extinction coefficient of AuNPs facilitates the colorimetric analysis of PSA in serum samples. This work is helpful for designing of other protease biosensors by matching specific peptide substrates. Keywords: colorimetric assay, gold nanoparticles, prostate-specific antigen, ascorbic acid, Cu2+ ion
format	article
author	Xia N Deng D Wang Y Fang C Li S
author_facet	Xia N Deng D Wang Y Fang C Li S
author_sort	Xia N
title	Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen
title_short	Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen
title_full	Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen
title_fullStr	Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen
title_full_unstemmed	Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen
title_sort	gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/5b3503e60e26475b853a118c938081b6
work_keys_str_mv	AT xian goldnanoparticlebasedcolorimetricmethodforthedetectionofprostatespecificantigen AT dengd goldnanoparticlebasedcolorimetricmethodforthedetectionofprostatespecificantigen AT wangy goldnanoparticlebasedcolorimetricmethodforthedetectionofprostatespecificantigen AT fangc goldnanoparticlebasedcolorimetricmethodforthedetectionofprostatespecificantigen AT lis goldnanoparticlebasedcolorimetricmethodforthedetectionofprostatespecificantigen
_version_	1718402896699064320

Gold nanoparticle-based colorimetric method for the detection of prostate-specific antigen

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