CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation

Wei Chen, Ning Wang, Minxue Lian Department of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shanxi Province, 710061, People’s Republic of ChinaCorrespondence: Minxue LianDepartment of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, No. 277 W...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen W, Wang N, Lian M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	glioblastoma circptk2 mir-23a maturation invasion migration Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/5b368315ffd04e4b9dbece894f3aeb70
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5b368315ffd04e4b9dbece894f3aeb70
record_format	dspace
spelling	oai:doaj.org-article:5b368315ffd04e4b9dbece894f3aeb702021-12-02T15:11:07ZCircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation1178-2021https://doaj.org/article/5b368315ffd04e4b9dbece894f3aeb702021-08-01T00:00:00Zhttps://www.dovepress.com/circrna-circptk2-might-suppress-cancer-cell-invasion-and-migration-of--peer-reviewed-fulltext-article-NDThttps://doaj.org/toc/1178-2021Wei Chen, Ning Wang, Minxue Lian Department of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shanxi Province, 710061, People’s Republic of ChinaCorrespondence: Minxue LianDepartment of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, No. 277 West Yanta Road, Xi’an City, Shanxi Province, 710061, People’s Republic of ChinaEmail MinxueLianShanxi@163.comBackground: CircRNA circPTK2 plays opposite roles in different cancers, while its role in glioblastoma is unknown. The aim of this study was to explore the involvement of circPTK2 in glioblastoma.Methods: Expression of circPTK2, mature miR-23a, and premature miR-23a in paired cancer and non-cancer tissues from glioblastoma patients (n = 60) was analyzed by RT-qPCR. Pearson’s correlation coefficient was used to analyze the correlations between gene expressions. The effects of circPTK2 overexpression on miR-23a maturation were analyzed by transfecting circPTK2 expression vector into glioblastoma cells, followed by determining the expression of mature miR-23a and premature miR-23a by RT-qPCR. Transwell assays were carried out to explore the role of circPTK2 and miR-23a in regulating glioblastoma cell invasion and migration.Results: We found that circPTK2 was downregulated in GBM and was inversely correlated with mature miR-23a, but not premature miR-23a. GBM cells transfected with circPTK2 expression vector showed significantly downregulated mature miR-23a, but not premature miR-23a. Transwell assay analysis showed that circPTK2 overexpression decreased cell invasion and migration, while miR-23a increased cell invasion and migration. Moreover, miR-23a overexpression reversed the inhibitory effects of circPTK2 overexpression on cell behaviors.Conclusion: CircPTK2 might suppress cancer cell invasion and migration by inhibiting the maturation of miR-23a.Keywords: glioblastoma, circPTK2, miR-23a, maturation, invasion, migrationChen WWang NLian MDove Medical Pressarticleglioblastomacircptk2mir-23amaturationinvasionmigrationNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 17, Pp 2767-2774 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioblastoma circptk2 mir-23a maturation invasion migration Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	glioblastoma circptk2 mir-23a maturation invasion migration Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Chen W Wang N Lian M CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation
description	Wei Chen, Ning Wang, Minxue Lian Department of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an City, Shanxi Province, 710061, People’s Republic of ChinaCorrespondence: Minxue LianDepartment of Neurosurgery, First Affiliated Hospital of Xi’an Jiaotong University, No. 277 West Yanta Road, Xi’an City, Shanxi Province, 710061, People’s Republic of ChinaEmail MinxueLianShanxi@163.comBackground: CircRNA circPTK2 plays opposite roles in different cancers, while its role in glioblastoma is unknown. The aim of this study was to explore the involvement of circPTK2 in glioblastoma.Methods: Expression of circPTK2, mature miR-23a, and premature miR-23a in paired cancer and non-cancer tissues from glioblastoma patients (n = 60) was analyzed by RT-qPCR. Pearson’s correlation coefficient was used to analyze the correlations between gene expressions. The effects of circPTK2 overexpression on miR-23a maturation were analyzed by transfecting circPTK2 expression vector into glioblastoma cells, followed by determining the expression of mature miR-23a and premature miR-23a by RT-qPCR. Transwell assays were carried out to explore the role of circPTK2 and miR-23a in regulating glioblastoma cell invasion and migration.Results: We found that circPTK2 was downregulated in GBM and was inversely correlated with mature miR-23a, but not premature miR-23a. GBM cells transfected with circPTK2 expression vector showed significantly downregulated mature miR-23a, but not premature miR-23a. Transwell assay analysis showed that circPTK2 overexpression decreased cell invasion and migration, while miR-23a increased cell invasion and migration. Moreover, miR-23a overexpression reversed the inhibitory effects of circPTK2 overexpression on cell behaviors.Conclusion: CircPTK2 might suppress cancer cell invasion and migration by inhibiting the maturation of miR-23a.Keywords: glioblastoma, circPTK2, miR-23a, maturation, invasion, migration
format	article
author	Chen W Wang N Lian M
author_facet	Chen W Wang N Lian M
author_sort	Chen W
title	CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation
title_short	CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation
title_full	CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation
title_fullStr	CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation
title_full_unstemmed	CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation
title_sort	circrna circptk2 might suppress cancer cell invasion and migration of glioblastoma by inhibiting mir-23a maturation
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/5b368315ffd04e4b9dbece894f3aeb70
work_keys_str_mv	AT chenw circrnacircptk2mightsuppresscancercellinvasionandmigrationofglioblastomabyinhibitingmir23amaturation AT wangn circrnacircptk2mightsuppresscancercellinvasionandmigrationofglioblastomabyinhibitingmir23amaturation AT lianm circrnacircptk2mightsuppresscancercellinvasionandmigrationofglioblastomabyinhibitingmir23amaturation
_version_	1718387629505904640

CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation

Ejemplares similares