TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ

Transforming growth factor (TGF)-β1 and mesenchymal stromal cells (MSCs) are two effective immunosuppressive agents for organ transplantation technology. This study aims to explore the molecular mechanism of TGF-β1-overexpressed MSCs on T cell immunosuppression. To achieve that, BM-MSCs were isolate...

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Autores principales:	Li Ruixue, Wang Renyong, Zhong Shijie, Asghar Farhan, Li Tiehan, Zhu Lei, Zhu Hong
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	tgf-β1 msc th17 treg immunosuppression Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5b38a0ff7a9945c1b2280d2955c4b82c
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spelling	oai:doaj.org-article:5b38a0ff7a9945c1b2280d2955c4b82c2021-12-05T14:10:42ZTGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ2391-541210.1515/biol-2021-0118https://doaj.org/article/5b38a0ff7a9945c1b2280d2955c4b82c2021-11-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0118https://doaj.org/toc/2391-5412Transforming growth factor (TGF)-β1 and mesenchymal stromal cells (MSCs) are two effective immunosuppressive agents for organ transplantation technology. This study aims to explore the molecular mechanism of TGF-β1-overexpressed MSCs on T cell immunosuppression. To achieve that, BM-MSCs were isolated from canine bone marrow, and their osteogenic differentiation and surface markers were detected. The TGF-β1 gene was transferred into lentivirus and modified MSCs (TGF-β1/MSCs) by lentivirus transfection. Furthermore, TGF-β1/MSCs were co-cultured with T cells to investigate their effect on differentiation and immune regulation. Results showed that TGF-β1/MSCs significantly downregulated the proportion of CD4+ CD8+ T cells in lymphocytes and significantly upregulated the proportion of CD4+ CD25+ T cells. Moreover, TGF-β1/MSCs significantly upregulated the expression of IL-10 in CD4+ T cells and downregulated the expression of IL-17A, IL-21, and IL-22. Meanwhile, interferon-γ (IFN-γ) neutralizing antibody blocked the effects of TGF-β1/MSCs on the differentiation inhibition of Th17. Overall, our results confirm the strong immunosuppressive effect of TGF-β1/MSCs in vitro and demonstrate that IFN-γ mediates the immunosuppressive effect of TGF-β1/MSC.Li RuixueWang RenyongZhong ShijieAsghar FarhanLi TiehanZhu LeiZhu HongDe Gruyterarticletgf-β1mscth17tregimmunosuppressionBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1193-1202 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tgf-β1 msc th17 treg immunosuppression Biology (General) QH301-705.5
spellingShingle	tgf-β1 msc th17 treg immunosuppression Biology (General) QH301-705.5 Li Ruixue Wang Renyong Zhong Shijie Asghar Farhan Li Tiehan Zhu Lei Zhu Hong TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ
description	Transforming growth factor (TGF)-β1 and mesenchymal stromal cells (MSCs) are two effective immunosuppressive agents for organ transplantation technology. This study aims to explore the molecular mechanism of TGF-β1-overexpressed MSCs on T cell immunosuppression. To achieve that, BM-MSCs were isolated from canine bone marrow, and their osteogenic differentiation and surface markers were detected. The TGF-β1 gene was transferred into lentivirus and modified MSCs (TGF-β1/MSCs) by lentivirus transfection. Furthermore, TGF-β1/MSCs were co-cultured with T cells to investigate their effect on differentiation and immune regulation. Results showed that TGF-β1/MSCs significantly downregulated the proportion of CD4+ CD8+ T cells in lymphocytes and significantly upregulated the proportion of CD4+ CD25+ T cells. Moreover, TGF-β1/MSCs significantly upregulated the expression of IL-10 in CD4+ T cells and downregulated the expression of IL-17A, IL-21, and IL-22. Meanwhile, interferon-γ (IFN-γ) neutralizing antibody blocked the effects of TGF-β1/MSCs on the differentiation inhibition of Th17. Overall, our results confirm the strong immunosuppressive effect of TGF-β1/MSCs in vitro and demonstrate that IFN-γ mediates the immunosuppressive effect of TGF-β1/MSC.
format	article
author	Li Ruixue Wang Renyong Zhong Shijie Asghar Farhan Li Tiehan Zhu Lei Zhu Hong
author_facet	Li Ruixue Wang Renyong Zhong Shijie Asghar Farhan Li Tiehan Zhu Lei Zhu Hong
author_sort	Li Ruixue
title	TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ
title_short	TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ
title_full	TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ
title_fullStr	TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ
title_full_unstemmed	TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ
title_sort	tgf-β1-overexpressing mesenchymal stem cells reciprocally regulate th17/treg cells by regulating the expression of ifn-γ
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/5b38a0ff7a9945c1b2280d2955c4b82c
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TGF-β1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ

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