Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging

Chih-Jen Wen1,*, Li-Wen Zhang2,*, Saleh A Al-Suwayeh3, Tzu-Chen Yen1, Jia-You Fang2,4 1Molecular Imaging Center, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; 2Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Gueishan, Taoyuan, Taiwan; 3Department o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wen CJ, Zhang LW, Al-Suwayeh SA, Yen TC, Fang JY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5b416cb1a3cd4d4c8a29117a6498210f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5b416cb1a3cd4d4c8a29117a6498210f
record_format	dspace
spelling	oai:doaj.org-article:5b416cb1a3cd4d4c8a29117a6498210f2021-12-02T07:23:00ZTheranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging1176-91141178-2013https://doaj.org/article/5b416cb1a3cd4d4c8a29117a6498210f2012-03-01T00:00:00Zhttp://www.dovepress.com/theranostic-liposomes-loaded-with-quantum-dots-and-apomorphine-for-bra-a9564https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Chih-Jen Wen1,, Li-Wen Zhang2,, Saleh A Al-Suwayeh3, Tzu-Chen Yen1, Jia-You Fang2,4 1Molecular Imaging Center, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; 2Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Gueishan, Taoyuan, Taiwan; 3Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; 4Department of Cosmetic Science, Chang Gung University of Science and Technology, Gueishan, Taoyuan, Taiwan *These authors contributed equally to this workAbstract: Quantum dots (QDs) and apomorphine were incorporated into liposomes to eliminate uptake by the liver and enhance brain targeting. We describe the preparation, physicochemical characterization, in vivo bioimaging, and brain endothelial cell uptake of the theranostic liposomes. QDs and the drug were mainly located in the bilayer membrane and inner core of the liposomes, respectively. Spherical vesicles with a mean diameter of ~140 nm were formed. QDs were completely encapsulated by the vesicles. Nearly 80% encapsulation percentage was achieved for apomorphine. A greater fluorescence intensity was observed in mouse brains treated with liposomes compared to free QDs. This result was further confirmed by ex vivo imaging of the organs. QD uptake by the heart and liver was reduced by liposomal incorporation. Apomorphine accumulation in the brain increased by 2.4-fold after this incorporation. According to a hyperspectral imaging analysis, multifunctional liposomes but not the aqueous solution carried QDs into the brain. Liposomes were observed to have been efficiently endocytosed into bEND3 cells. The mechanisms involved in the cellular uptake were clathrin- and caveola-mediated endocytosis, which were energy-dependent. To the best of our knowledge, our group is the first to develop liposomes with a QD-drug hybrid for the aim of imaging and treating brain disorders.Keywords: liposomes, quantum dots, apomorphine, brain targeting, bioimagingWen CJZhang LWAl-Suwayeh SAYen TCFang JYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1599-1611 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wen CJ Zhang LW Al-Suwayeh SA Yen TC Fang JY Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging
description	Chih-Jen Wen1,, Li-Wen Zhang2,, Saleh A Al-Suwayeh3, Tzu-Chen Yen1, Jia-You Fang2,4 1Molecular Imaging Center, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; 2Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Gueishan, Taoyuan, Taiwan; 3Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; 4Department of Cosmetic Science, Chang Gung University of Science and Technology, Gueishan, Taoyuan, Taiwan *These authors contributed equally to this workAbstract: Quantum dots (QDs) and apomorphine were incorporated into liposomes to eliminate uptake by the liver and enhance brain targeting. We describe the preparation, physicochemical characterization, in vivo bioimaging, and brain endothelial cell uptake of the theranostic liposomes. QDs and the drug were mainly located in the bilayer membrane and inner core of the liposomes, respectively. Spherical vesicles with a mean diameter of ~140 nm were formed. QDs were completely encapsulated by the vesicles. Nearly 80% encapsulation percentage was achieved for apomorphine. A greater fluorescence intensity was observed in mouse brains treated with liposomes compared to free QDs. This result was further confirmed by ex vivo imaging of the organs. QD uptake by the heart and liver was reduced by liposomal incorporation. Apomorphine accumulation in the brain increased by 2.4-fold after this incorporation. According to a hyperspectral imaging analysis, multifunctional liposomes but not the aqueous solution carried QDs into the brain. Liposomes were observed to have been efficiently endocytosed into bEND3 cells. The mechanisms involved in the cellular uptake were clathrin- and caveola-mediated endocytosis, which were energy-dependent. To the best of our knowledge, our group is the first to develop liposomes with a QD-drug hybrid for the aim of imaging and treating brain disorders.Keywords: liposomes, quantum dots, apomorphine, brain targeting, bioimaging
format	article
author	Wen CJ Zhang LW Al-Suwayeh SA Yen TC Fang JY
author_facet	Wen CJ Zhang LW Al-Suwayeh SA Yen TC Fang JY
author_sort	Wen CJ
title	Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging
title_short	Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging
title_full	Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging
title_fullStr	Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging
title_full_unstemmed	Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging
title_sort	theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/5b416cb1a3cd4d4c8a29117a6498210f
work_keys_str_mv	AT wencj theranosticliposomesloadedwithquantumdotsandapomorphineforbraintargetingandbioimaging AT zhanglw theranosticliposomesloadedwithquantumdotsandapomorphineforbraintargetingandbioimaging AT alsuwayehsa theranosticliposomesloadedwithquantumdotsandapomorphineforbraintargetingandbioimaging AT yentc theranosticliposomesloadedwithquantumdotsandapomorphineforbraintargetingandbioimaging AT fangjy theranosticliposomesloadedwithquantumdotsandapomorphineforbraintargetingandbioimaging
_version_	1718399457147486208

Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging

Ejemplares similares