The sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking

Abstract Magnetic particle imaging (MPI) and fluorine-19 (19F) MRI produce images which allow for quantification of labeled cells. MPI is an emerging instrument for cell tracking, which is expected to have superior sensitivity compared to 19F MRI. Our objective is to assess the cellular sensitivity...

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Autores principales:	Olivia C. Sehl, Paula J. Foster
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b499c4bc47e4890b6d7cc4b1ee8a021
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spelling	oai:doaj.org-article:5b499c4bc47e4890b6d7cc4b1ee8a0212021-11-14T12:20:30ZThe sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking10.1038/s41598-021-01642-32045-2322https://doaj.org/article/5b499c4bc47e4890b6d7cc4b1ee8a0212021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01642-3https://doaj.org/toc/2045-2322Abstract Magnetic particle imaging (MPI) and fluorine-19 (19F) MRI produce images which allow for quantification of labeled cells. MPI is an emerging instrument for cell tracking, which is expected to have superior sensitivity compared to 19F MRI. Our objective is to assess the cellular sensitivity of MPI and 19F MRI for detection of mesenchymal stem cells (MSC) and breast cancer cells. Cells were labeled with ferucarbotran or perfluoropolyether, for imaging on a preclinical MPI system or 3 Tesla clinical MRI, respectively. Using the same imaging time, as few as 4000 MSC (76 ng iron) and 8000 breast cancer cells (74 ng iron) were reliably detected with MPI, and 256,000 MSC (9.01 × 1016 19F atoms) were detected with 19F MRI, with SNR > 5. MPI has the potential to be more sensitive than 19F MRI for cell tracking. In vivo sensitivity with MPI and 19F MRI was evaluated by imaging MSC that were administered by different routes. In vivo imaging revealed reduced sensitivity compared to ex vivo cell pellets of the same cell number. We attribute reduced MPI and 19F MRI cell detection in vivo to the effect of cell dispersion among other factors, which are described.Olivia C. SehlPaula J. FosterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olivia C. Sehl Paula J. Foster The sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking
description	Abstract Magnetic particle imaging (MPI) and fluorine-19 (19F) MRI produce images which allow for quantification of labeled cells. MPI is an emerging instrument for cell tracking, which is expected to have superior sensitivity compared to 19F MRI. Our objective is to assess the cellular sensitivity of MPI and 19F MRI for detection of mesenchymal stem cells (MSC) and breast cancer cells. Cells were labeled with ferucarbotran or perfluoropolyether, for imaging on a preclinical MPI system or 3 Tesla clinical MRI, respectively. Using the same imaging time, as few as 4000 MSC (76 ng iron) and 8000 breast cancer cells (74 ng iron) were reliably detected with MPI, and 256,000 MSC (9.01 × 1016 19F atoms) were detected with 19F MRI, with SNR > 5. MPI has the potential to be more sensitive than 19F MRI for cell tracking. In vivo sensitivity with MPI and 19F MRI was evaluated by imaging MSC that were administered by different routes. In vivo imaging revealed reduced sensitivity compared to ex vivo cell pellets of the same cell number. We attribute reduced MPI and 19F MRI cell detection in vivo to the effect of cell dispersion among other factors, which are described.
format	article
author	Olivia C. Sehl Paula J. Foster
author_facet	Olivia C. Sehl Paula J. Foster
author_sort	Olivia C. Sehl
title	The sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking
title_short	The sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking
title_full	The sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking
title_fullStr	The sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking
title_full_unstemmed	The sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking
title_sort	sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5b499c4bc47e4890b6d7cc4b1ee8a021
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The sensitivity of magnetic particle imaging and fluorine-19 magnetic resonance imaging for cell tracking

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