An evaluation of commercial fluorescent bead-based luminex cytokine assays.

The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 microl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual...

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Autores principales:	Joel Fleury Djoba Siawaya, Teri Roberts, Chantal Babb, Gillian Black, Hawa Jande Golakai, Kim Stanley, Nchinya Bennedict Bapela, Eileen Hoal, Shreemanta Parida, Paul van Helden, Gerhard Walzl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b4c2211b2714b369b6d09b19ec33357
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spelling	oai:doaj.org-article:5b4c2211b2714b369b6d09b19ec333572021-11-25T06:11:47ZAn evaluation of commercial fluorescent bead-based luminex cytokine assays.1932-620310.1371/journal.pone.0002535https://doaj.org/article/5b4c2211b2714b369b6d09b19ec333572008-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18596971/?tool=EBIhttps://doaj.org/toc/1932-6203The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 microl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex cytokine fluorescent bead-based immunoassays (Bio-Rad's Cytokine 17-plex kit; LINCO Inc's 29-plex kit; and RnD System's Fluorokine-Multi Analyte Profiling (MAP) base kit A and B). The LINCO Inc kit was found to be the most sensitive assay for measuring concentrations of multiple recombinant cytokines in samples that had been spiked with serial dilutions of the standard provided by the manufacturer, followed respectively by the RnD Fluorokine-(MAP) and Bio-Rad 17-plex kits. A positive correlation was found in the levels of IFN-gamma measured in antigen stimulated whole blood culture supernatants by the LINCO Inc 29-plex, RnD Fluorokine-(MAP) and RnD system IFN-gamma Quantikine ELISA kits across a panel of controls and stimulated samples. Researchers should take the limitation of such multiplexed assays into account when planning experiments and the most appropriate use for these tests may currently be as screening tools for the selection of promising markers for analysis by more sensitive techniques.Joel Fleury Djoba SiawayaTeri RobertsChantal BabbGillian BlackHawa Jande GolakaiKim StanleyNchinya Bennedict BapelaEileen HoalShreemanta ParidaPaul van HeldenGerhard WalzlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 7, p e2535 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joel Fleury Djoba Siawaya Teri Roberts Chantal Babb Gillian Black Hawa Jande Golakai Kim Stanley Nchinya Bennedict Bapela Eileen Hoal Shreemanta Parida Paul van Helden Gerhard Walzl An evaluation of commercial fluorescent bead-based luminex cytokine assays.
description	The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 microl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex cytokine fluorescent bead-based immunoassays (Bio-Rad's Cytokine 17-plex kit; LINCO Inc's 29-plex kit; and RnD System's Fluorokine-Multi Analyte Profiling (MAP) base kit A and B). The LINCO Inc kit was found to be the most sensitive assay for measuring concentrations of multiple recombinant cytokines in samples that had been spiked with serial dilutions of the standard provided by the manufacturer, followed respectively by the RnD Fluorokine-(MAP) and Bio-Rad 17-plex kits. A positive correlation was found in the levels of IFN-gamma measured in antigen stimulated whole blood culture supernatants by the LINCO Inc 29-plex, RnD Fluorokine-(MAP) and RnD system IFN-gamma Quantikine ELISA kits across a panel of controls and stimulated samples. Researchers should take the limitation of such multiplexed assays into account when planning experiments and the most appropriate use for these tests may currently be as screening tools for the selection of promising markers for analysis by more sensitive techniques.
format	article
author	Joel Fleury Djoba Siawaya Teri Roberts Chantal Babb Gillian Black Hawa Jande Golakai Kim Stanley Nchinya Bennedict Bapela Eileen Hoal Shreemanta Parida Paul van Helden Gerhard Walzl
author_facet	Joel Fleury Djoba Siawaya Teri Roberts Chantal Babb Gillian Black Hawa Jande Golakai Kim Stanley Nchinya Bennedict Bapela Eileen Hoal Shreemanta Parida Paul van Helden Gerhard Walzl
author_sort	Joel Fleury Djoba Siawaya
title	An evaluation of commercial fluorescent bead-based luminex cytokine assays.
title_short	An evaluation of commercial fluorescent bead-based luminex cytokine assays.
title_full	An evaluation of commercial fluorescent bead-based luminex cytokine assays.
title_fullStr	An evaluation of commercial fluorescent bead-based luminex cytokine assays.
title_full_unstemmed	An evaluation of commercial fluorescent bead-based luminex cytokine assays.
title_sort	evaluation of commercial fluorescent bead-based luminex cytokine assays.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/5b4c2211b2714b369b6d09b19ec33357
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An evaluation of commercial fluorescent bead-based luminex cytokine assays.

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