Profiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.

Dodders are among the most important parasitic plants that cause serious yield losses in crop plants. In this report, we sought to unveil the genetic basis of dodder parasitism by profiling the trancriptomes of Cuscuta pentagona and C. suaveolens, two of the most common dodder species using a next-g...

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Autores principales:	Linjian Jiang, Asela J Wijeratne, Saranga Wijeratne, Martina Fraga, Tea Meulia, Doug Doohan, Zhaohu Li, Feng Qu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b4d379d7427486295027abe405cc334
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spelling	oai:doaj.org-article:5b4d379d7427486295027abe405cc3342021-11-18T08:44:24ZProfiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.1932-620310.1371/journal.pone.0081389https://doaj.org/article/5b4d379d7427486295027abe405cc3342013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312295/?tool=EBIhttps://doaj.org/toc/1932-6203Dodders are among the most important parasitic plants that cause serious yield losses in crop plants. In this report, we sought to unveil the genetic basis of dodder parasitism by profiling the trancriptomes of Cuscuta pentagona and C. suaveolens, two of the most common dodder species using a next-generation RNA sequencing platform. De novo assembly of the sequence reads resulted in more than 46,000 isotigs and contigs (collectively referred to as expressed sequence tags or ESTs) for each species, with more than half of them predicted to encode proteins that share significant sequence similarities with known proteins of non-parasitic plants. Comparing our datasets with transcriptomes of 12 other fully sequenced plant species confirmed a close evolutionary relationship between dodder and tomato. Using a rigorous set of filtering parameters, we were able to identify seven pairs of ESTs that appear to be shared exclusively by parasitic plants, thus providing targets for tailored management approaches. In addition, we also discovered ESTs with sequences similarities to known plant viruses, including cryptic viruses, in the dodder sequence assemblies. Together this study represents the first comprehensive transcriptome profiling of parasitic plants in the Cuscuta genus, and is expected to contribute to our understanding of the molecular mechanisms of parasitic plant-host plant interactions.Linjian JiangAsela J WijeratneSaranga WijeratneMartina FragaTea MeuliaDoug DoohanZhaohu LiFeng QuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81389 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Linjian Jiang Asela J Wijeratne Saranga Wijeratne Martina Fraga Tea Meulia Doug Doohan Zhaohu Li Feng Qu Profiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.
description	Dodders are among the most important parasitic plants that cause serious yield losses in crop plants. In this report, we sought to unveil the genetic basis of dodder parasitism by profiling the trancriptomes of Cuscuta pentagona and C. suaveolens, two of the most common dodder species using a next-generation RNA sequencing platform. De novo assembly of the sequence reads resulted in more than 46,000 isotigs and contigs (collectively referred to as expressed sequence tags or ESTs) for each species, with more than half of them predicted to encode proteins that share significant sequence similarities with known proteins of non-parasitic plants. Comparing our datasets with transcriptomes of 12 other fully sequenced plant species confirmed a close evolutionary relationship between dodder and tomato. Using a rigorous set of filtering parameters, we were able to identify seven pairs of ESTs that appear to be shared exclusively by parasitic plants, thus providing targets for tailored management approaches. In addition, we also discovered ESTs with sequences similarities to known plant viruses, including cryptic viruses, in the dodder sequence assemblies. Together this study represents the first comprehensive transcriptome profiling of parasitic plants in the Cuscuta genus, and is expected to contribute to our understanding of the molecular mechanisms of parasitic plant-host plant interactions.
format	article
author	Linjian Jiang Asela J Wijeratne Saranga Wijeratne Martina Fraga Tea Meulia Doug Doohan Zhaohu Li Feng Qu
author_facet	Linjian Jiang Asela J Wijeratne Saranga Wijeratne Martina Fraga Tea Meulia Doug Doohan Zhaohu Li Feng Qu
author_sort	Linjian Jiang
title	Profiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.
title_short	Profiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.
title_full	Profiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.
title_fullStr	Profiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.
title_full_unstemmed	Profiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.
title_sort	profiling mrnas of two cuscuta species reveals possible candidate transcripts shared by parasitic plants.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5b4d379d7427486295027abe405cc334
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Profiling mRNAs of two Cuscuta species reveals possible candidate transcripts shared by parasitic plants.

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