De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.

The manner in which inserted foreign coding sequences become transcriptionally activated and fixed in the plant genome is poorly understood. To examine such processes of gene evolution, we performed an artificial evolutionary experiment in Arabidopsis thaliana. As a model of gene-birth events, we in...

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Autores principales:	Takayuki Hata, Naoto Takada, Chihiro Hayakawa, Mei Kazama, Tomohiro Uchikoba, Makoto Tachikawa, Mitsuhiro Matsuo, Soichirou Satoh, Junichi Obokata
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b5fce91df984b8aa9a29d1dc4665808
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spelling	oai:doaj.org-article:5b5fce91df984b8aa9a29d1dc46658082021-12-02T20:07:11ZDe novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.1932-620310.1371/journal.pone.0252674https://doaj.org/article/5b5fce91df984b8aa9a29d1dc46658082021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252674https://doaj.org/toc/1932-6203The manner in which inserted foreign coding sequences become transcriptionally activated and fixed in the plant genome is poorly understood. To examine such processes of gene evolution, we performed an artificial evolutionary experiment in Arabidopsis thaliana. As a model of gene-birth events, we introduced a promoterless coding sequence of the firefly luciferase (LUC) gene and established 386 T2-generation transgenic lines. Among them, we determined the individual LUC insertion loci in 76 lines and found that one-third of them were transcribed de novo even in the intergenic or inherently unexpressed regions. In the transcribed lines, transcription-related chromatin marks were detected across the newly activated transcribed regions. These results agreed with our previous findings in A. thaliana cultured cells under a similar experimental scheme. A comparison of the results of the T2-plant and cultured cell experiments revealed that the de novo-activated transcription concomitant with local chromatin remodelling was inheritable. During one-generation inheritance, it seems likely that the transcription activities of the LUC inserts trapped by the endogenous genes/transcripts became stronger, while those of de novo transcription in the intergenic/untranscribed regions became weaker. These findings may offer a clue for the elucidation of the mechanism by which inserted foreign coding sequences become transcriptionally activated and fixed in the plant genome.Takayuki HataNaoto TakadaChihiro HayakawaMei KazamaTomohiro UchikobaMakoto TachikawaMitsuhiro MatsuoSoichirou SatohJunichi ObokataPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252674 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takayuki Hata Naoto Takada Chihiro Hayakawa Mei Kazama Tomohiro Uchikoba Makoto Tachikawa Mitsuhiro Matsuo Soichirou Satoh Junichi Obokata De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.
description	The manner in which inserted foreign coding sequences become transcriptionally activated and fixed in the plant genome is poorly understood. To examine such processes of gene evolution, we performed an artificial evolutionary experiment in Arabidopsis thaliana. As a model of gene-birth events, we introduced a promoterless coding sequence of the firefly luciferase (LUC) gene and established 386 T2-generation transgenic lines. Among them, we determined the individual LUC insertion loci in 76 lines and found that one-third of them were transcribed de novo even in the intergenic or inherently unexpressed regions. In the transcribed lines, transcription-related chromatin marks were detected across the newly activated transcribed regions. These results agreed with our previous findings in A. thaliana cultured cells under a similar experimental scheme. A comparison of the results of the T2-plant and cultured cell experiments revealed that the de novo-activated transcription concomitant with local chromatin remodelling was inheritable. During one-generation inheritance, it seems likely that the transcription activities of the LUC inserts trapped by the endogenous genes/transcripts became stronger, while those of de novo transcription in the intergenic/untranscribed regions became weaker. These findings may offer a clue for the elucidation of the mechanism by which inserted foreign coding sequences become transcriptionally activated and fixed in the plant genome.
format	article
author	Takayuki Hata Naoto Takada Chihiro Hayakawa Mei Kazama Tomohiro Uchikoba Makoto Tachikawa Mitsuhiro Matsuo Soichirou Satoh Junichi Obokata
author_facet	Takayuki Hata Naoto Takada Chihiro Hayakawa Mei Kazama Tomohiro Uchikoba Makoto Tachikawa Mitsuhiro Matsuo Soichirou Satoh Junichi Obokata
author_sort	Takayuki Hata
title	De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.
title_short	De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.
title_full	De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.
title_fullStr	De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.
title_full_unstemmed	De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.
title_sort	de novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5b5fce91df984b8aa9a29d1dc4665808
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De novo activated transcription of inserted foreign coding sequences is inheritable in the plant genome.

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