Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments

Jae-Kyo Jeong,1 You-Jin Lee,1 Seung Yol Jeong,2,3 Sooyeon Jeong,2 Geon-Woong Lee,2 Sang-Youel Park1 1Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksan, 2Nano Hybrid Technology Research Center, Korea Electrotechnology Research Institute (KERI), Changwon...

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Autores principales:	Jeong JK, Lee YJ, Jeong SY, Jeong S, Lee GW, Park SY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Graphene oxide neuroprotection autophagy prion protein mitochondria Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5b63750f4c3647a38d7096102ac5502f
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spelling	oai:doaj.org-article:5b63750f4c3647a38d7096102ac5502f2021-12-02T05:04:24ZAutophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments1178-2013https://doaj.org/article/5b63750f4c3647a38d7096102ac5502f2017-11-01T00:00:00Zhttps://www.dovepress.com/autophagic-flux-induced-by-graphene-oxide-has-a-neuroprotective-effect-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jae-Kyo Jeong,1 You-Jin Lee,1 Seung Yol Jeong,2,3 Sooyeon Jeong,2 Geon-Woong Lee,2 Sang-Youel Park1 1Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksan, 2Nano Hybrid Technology Research Center, Korea Electrotechnology Research Institute (KERI), Changwon, 3Department of Electrical Functionality Material Engineering, University of Science and Technology (UST), Daejon, Republic of Korea Abstract: Graphene oxide (GO) is a nanomaterial with newly developing biological applications. Autophagy is an intracellular degradation system that has been associated with the progression of neurodegenerative disorders. Although induction of autophagic flux by GO has been reported, the underlying signaling pathway in neurodegenerative disorders and how this is involved in neuroprotection remain obscure. We show that GO itself activates autophagic flux in neuronal cells and confers a neuroprotective effect against prion protein (PrP) (106–126)-mediated neurotoxicity. GO can be detected in SK-N-SH neuronal cells, where it triggers autophagic flux signaling. GO-induced autophagic flux prevented PrP (106–126)-induced neurotoxicity in SK-N-SH cells. Moreover, inactivation of autophagic flux blocked GO-induced neuroprotection against prion-mediated mitochondrial neurotoxicity. This is the first study to demonstrate that GO regulates autophagic flux in neuronal cells, and that activation of autophagic flux signals, induced by GO, plays a neuroprotective role against prion-mediated mitochondrial neurotoxicity. These results suggest that the nanomaterial GO may be used to activate autophagic flux and could be used in neuroprotective strategies for treatment of neurodegenerative disorders, including prion diseases. Keywords: graphene oxide, neuroprotection, autophagy, prion protein, mitochondriaJeong JKLee YJJeong SYJeong SLee GWPark SYDove Medical PressarticleGraphene oxideneuroprotectionautophagyprion proteinmitochondriaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8143-8158 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Graphene oxide neuroprotection autophagy prion protein mitochondria Medicine (General) R5-920
spellingShingle	Graphene oxide neuroprotection autophagy prion protein mitochondria Medicine (General) R5-920 Jeong JK Lee YJ Jeong SY Jeong S Lee GW Park SY Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments
description	Jae-Kyo Jeong,1 You-Jin Lee,1 Seung Yol Jeong,2,3 Sooyeon Jeong,2 Geon-Woong Lee,2 Sang-Youel Park1 1Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksan, 2Nano Hybrid Technology Research Center, Korea Electrotechnology Research Institute (KERI), Changwon, 3Department of Electrical Functionality Material Engineering, University of Science and Technology (UST), Daejon, Republic of Korea Abstract: Graphene oxide (GO) is a nanomaterial with newly developing biological applications. Autophagy is an intracellular degradation system that has been associated with the progression of neurodegenerative disorders. Although induction of autophagic flux by GO has been reported, the underlying signaling pathway in neurodegenerative disorders and how this is involved in neuroprotection remain obscure. We show that GO itself activates autophagic flux in neuronal cells and confers a neuroprotective effect against prion protein (PrP) (106–126)-mediated neurotoxicity. GO can be detected in SK-N-SH neuronal cells, where it triggers autophagic flux signaling. GO-induced autophagic flux prevented PrP (106–126)-induced neurotoxicity in SK-N-SH cells. Moreover, inactivation of autophagic flux blocked GO-induced neuroprotection against prion-mediated mitochondrial neurotoxicity. This is the first study to demonstrate that GO regulates autophagic flux in neuronal cells, and that activation of autophagic flux signals, induced by GO, plays a neuroprotective role against prion-mediated mitochondrial neurotoxicity. These results suggest that the nanomaterial GO may be used to activate autophagic flux and could be used in neuroprotective strategies for treatment of neurodegenerative disorders, including prion diseases. Keywords: graphene oxide, neuroprotection, autophagy, prion protein, mitochondria
format	article
author	Jeong JK Lee YJ Jeong SY Jeong S Lee GW Park SY
author_facet	Jeong JK Lee YJ Jeong SY Jeong S Lee GW Park SY
author_sort	Jeong JK
title	Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments
title_short	Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments
title_full	Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments
title_fullStr	Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments
title_full_unstemmed	Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments
title_sort	autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/5b63750f4c3647a38d7096102ac5502f
work_keys_str_mv	AT jeongjk autophagicfluxinducedbygrapheneoxidehasaneuroprotectiveeffectagainsthumanprionproteinfragments AT leeyj autophagicfluxinducedbygrapheneoxidehasaneuroprotectiveeffectagainsthumanprionproteinfragments AT jeongsy autophagicfluxinducedbygrapheneoxidehasaneuroprotectiveeffectagainsthumanprionproteinfragments AT jeongs autophagicfluxinducedbygrapheneoxidehasaneuroprotectiveeffectagainsthumanprionproteinfragments AT leegw autophagicfluxinducedbygrapheneoxidehasaneuroprotectiveeffectagainsthumanprionproteinfragments AT parksy autophagicfluxinducedbygrapheneoxidehasaneuroprotectiveeffectagainsthumanprionproteinfragments
_version_	1718400651122180096

Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments

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