Comparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells

Abstract During 2020, understanding the molecular mechanism of SARS-CoV-2 infection (the cause of COVID-19) became a scientific priority due to the devastating effects of the COVID-19. Many researchers have studied the effect of this viral infection on lung epithelial transcriptomes and deposited da...

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Autores principales:	Darshan S. Chandrashekar, Mohammad Athar, Upender Manne, Sooryanarayana Varambally
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b6637bc86d84b4fb3d9d6a089a07581
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spelling	oai:doaj.org-article:5b6637bc86d84b4fb3d9d6a089a075812021-12-02T16:27:45ZComparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells10.1038/s41598-021-95733-w2045-2322https://doaj.org/article/5b6637bc86d84b4fb3d9d6a089a075812021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95733-whttps://doaj.org/toc/2045-2322Abstract During 2020, understanding the molecular mechanism of SARS-CoV-2 infection (the cause of COVID-19) became a scientific priority due to the devastating effects of the COVID-19. Many researchers have studied the effect of this viral infection on lung epithelial transcriptomes and deposited data in public repositories. Comprehensive analysis of such data could pave the way for development of efficient vaccines and effective drugs. In the current study, we obtained high-throughput gene expression data associated with human lung epithelial cells infected with respiratory viruses such as SARS-CoV-2, SARS, H1N1, avian influenza, rhinovirus and Dhori, then performed comparative transcriptome analysis to identify SARS-CoV-2 exclusive genes. The analysis yielded seven SARS-CoV-2 specific genes including CSF2 [GM-CSF] (colony-stimulating factor 2) and calcium-binding proteins (such as S100A8 and S100A9), which are known to be involved in respiratory diseases. The analyses showed that genes involved in inflammation are commonly altered by infection of SARS-CoV-2 and influenza viruses. Furthermore, results of protein–protein interaction analyses were consistent with a functional role of CSF2 and S100A9 in COVID-19 disease. In conclusion, our analysis revealed cellular genes associated with SARS-CoV-2 infection of the human lung epithelium; these are potential therapeutic targets.Darshan S. ChandrashekarMohammad AtharUpender ManneSooryanarayana VaramballyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Darshan S. Chandrashekar Mohammad Athar Upender Manne Sooryanarayana Varambally Comparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells
description	Abstract During 2020, understanding the molecular mechanism of SARS-CoV-2 infection (the cause of COVID-19) became a scientific priority due to the devastating effects of the COVID-19. Many researchers have studied the effect of this viral infection on lung epithelial transcriptomes and deposited data in public repositories. Comprehensive analysis of such data could pave the way for development of efficient vaccines and effective drugs. In the current study, we obtained high-throughput gene expression data associated with human lung epithelial cells infected with respiratory viruses such as SARS-CoV-2, SARS, H1N1, avian influenza, rhinovirus and Dhori, then performed comparative transcriptome analysis to identify SARS-CoV-2 exclusive genes. The analysis yielded seven SARS-CoV-2 specific genes including CSF2 [GM-CSF] (colony-stimulating factor 2) and calcium-binding proteins (such as S100A8 and S100A9), which are known to be involved in respiratory diseases. The analyses showed that genes involved in inflammation are commonly altered by infection of SARS-CoV-2 and influenza viruses. Furthermore, results of protein–protein interaction analyses were consistent with a functional role of CSF2 and S100A9 in COVID-19 disease. In conclusion, our analysis revealed cellular genes associated with SARS-CoV-2 infection of the human lung epithelium; these are potential therapeutic targets.
format	article
author	Darshan S. Chandrashekar Mohammad Athar Upender Manne Sooryanarayana Varambally
author_facet	Darshan S. Chandrashekar Mohammad Athar Upender Manne Sooryanarayana Varambally
author_sort	Darshan S. Chandrashekar
title	Comparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells
title_short	Comparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells
title_full	Comparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells
title_fullStr	Comparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells
title_full_unstemmed	Comparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells
title_sort	comparative transcriptome analyses reveal genes associated with sars-cov-2 infection of human lung epithelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5b6637bc86d84b4fb3d9d6a089a07581
work_keys_str_mv	AT darshanschandrashekar comparativetranscriptomeanalysesrevealgenesassociatedwithsarscov2infectionofhumanlungepithelialcells AT mohammadathar comparativetranscriptomeanalysesrevealgenesassociatedwithsarscov2infectionofhumanlungepithelialcells AT upendermanne comparativetranscriptomeanalysesrevealgenesassociatedwithsarscov2infectionofhumanlungepithelialcells AT sooryanarayanavarambally comparativetranscriptomeanalysesrevealgenesassociatedwithsarscov2infectionofhumanlungepithelialcells
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Comparative transcriptome analyses reveal genes associated with SARS-CoV-2 infection of human lung epithelial cells

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