Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p

Nasopharyngeal carcinoma (NPC) is a typical type of malignant tumor. This research paper aims to study the function and mechanism of long non-coding RNA lung adenocarcinoma related transcript 1 (lncRNA-LUADT1) in the progression of NPC. In this study, the expressions of lncRNA-LUADT1, miR-1207-5p, a...

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Autores principales:	Ning Jiang, Lijun Zhao, Dan Zong, Li Yin, Lirong Wu, Cheng Chen, Xue Song, Qian Zhang, Xuesong Jiang, Xia He, Jifeng Feng
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	nasopharyngeal carcinoma lncrna-luadt1 mir-1207-5p tead1 hippo/yap signaling pathway Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5b66c63018244edc991ca899c7c0d8c0
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spelling	oai:doaj.org-article:5b66c63018244edc991ca899c7c0d8c02021-11-11T14:23:43ZLong non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p2165-59792165-598710.1080/21655979.2021.2001952https://doaj.org/article/5b66c63018244edc991ca899c7c0d8c02021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2001952https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Nasopharyngeal carcinoma (NPC) is a typical type of malignant tumor. This research paper aims to study the function and mechanism of long non-coding RNA lung adenocarcinoma related transcript 1 (lncRNA-LUADT1) in the progression of NPC. In this study, the expressions of lncRNA-LUADT1, miR-1207-5p, and TEAD1 in NPC tissues and cell lines were detected by RT-qPCR. Initially, the expression of lncRNA-LUADT1 and TEAD1 were significantly up-regulated in NPC tissues and cells, while miR-1207-5p was significantly down-regulated. Next, miR-1207-5p was confirmed to bind to lncRNA-LUADT1 or TEAD1 by bioinformatics and luciferase reporter assay. In addition, after interfering with lncRNA-LUADT1 expression, experiments of CCK8, EDU staining, and Transwell invasion were used to detect proliferation, invasion, and migration of NPC cells. The results showed that interfering with lncRNA-LUADT1 expression could inhibit the proliferation, invasion, and migration of NPC cells. Western blot showed that lncRNA-LUADT1 knockdown significantly decreased the expression of Hippo/YAP pathway protein (YAP1 and TAZ). However, interfering with the expression of miR-1207-5p reversed these results. In addition, the nude mouse tumor formation experiment suggested that low-expressed lncRNA-LUADT1 reduced the volume and weight of tumor tissues. In summary, lncRNA-LUADT1 down-regulation could inhibit NPC cell proliferation and invasion, which may be achieved through regulating miR-1207-5p expression and affecting TEAD1 expression, thus inhibiting the activation of Hippo/YAP signaling pathway.Ning JiangLijun ZhaoDan ZongLi YinLirong WuCheng ChenXue SongQian ZhangXuesong JiangXia HeJifeng FengTaylor & Francis Grouparticlenasopharyngeal carcinomalncrna-luadt1mir-1207-5ptead1hippo/yap signaling pathwayBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nasopharyngeal carcinoma lncrna-luadt1 mir-1207-5p tead1 hippo/yap signaling pathway Biotechnology TP248.13-248.65
spellingShingle	nasopharyngeal carcinoma lncrna-luadt1 mir-1207-5p tead1 hippo/yap signaling pathway Biotechnology TP248.13-248.65 Ning Jiang Lijun Zhao Dan Zong Li Yin Lirong Wu Cheng Chen Xue Song Qian Zhang Xuesong Jiang Xia He Jifeng Feng Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p
description	Nasopharyngeal carcinoma (NPC) is a typical type of malignant tumor. This research paper aims to study the function and mechanism of long non-coding RNA lung adenocarcinoma related transcript 1 (lncRNA-LUADT1) in the progression of NPC. In this study, the expressions of lncRNA-LUADT1, miR-1207-5p, and TEAD1 in NPC tissues and cell lines were detected by RT-qPCR. Initially, the expression of lncRNA-LUADT1 and TEAD1 were significantly up-regulated in NPC tissues and cells, while miR-1207-5p was significantly down-regulated. Next, miR-1207-5p was confirmed to bind to lncRNA-LUADT1 or TEAD1 by bioinformatics and luciferase reporter assay. In addition, after interfering with lncRNA-LUADT1 expression, experiments of CCK8, EDU staining, and Transwell invasion were used to detect proliferation, invasion, and migration of NPC cells. The results showed that interfering with lncRNA-LUADT1 expression could inhibit the proliferation, invasion, and migration of NPC cells. Western blot showed that lncRNA-LUADT1 knockdown significantly decreased the expression of Hippo/YAP pathway protein (YAP1 and TAZ). However, interfering with the expression of miR-1207-5p reversed these results. In addition, the nude mouse tumor formation experiment suggested that low-expressed lncRNA-LUADT1 reduced the volume and weight of tumor tissues. In summary, lncRNA-LUADT1 down-regulation could inhibit NPC cell proliferation and invasion, which may be achieved through regulating miR-1207-5p expression and affecting TEAD1 expression, thus inhibiting the activation of Hippo/YAP signaling pathway.
format	article
author	Ning Jiang Lijun Zhao Dan Zong Li Yin Lirong Wu Cheng Chen Xue Song Qian Zhang Xuesong Jiang Xia He Jifeng Feng
author_facet	Ning Jiang Lijun Zhao Dan Zong Li Yin Lirong Wu Cheng Chen Xue Song Qian Zhang Xuesong Jiang Xia He Jifeng Feng
author_sort	Ning Jiang
title	Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p
title_short	Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p
title_full	Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p
title_fullStr	Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p
title_full_unstemmed	Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p
title_sort	long non-coding rna luadt1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating mir-1207-5p
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/5b66c63018244edc991ca899c7c0d8c0
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Long non-coding RNA LUADT1 promotes nasopharyngeal carcinoma cell proliferation and invasion by downregulating miR-1207-5p

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