Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers

Abstract Microfluidic focusing of particles (both synthetic and biological), which enables precise control over the positions of particles in a tightly focused stream, is a prerequisite step for the downstream processing, such as detection, trapping and separation. In this study, we propose a novel...

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Autores principales:	Tianlong Zhang, Misuzu Namoto, Kazunori Okano, Eri Akita, Norihiro Teranishi, Tao Tang, Dian Anggraini, Yansheng Hao, Yo Tanaka, David Inglis, Yaxiaer Yalikun, Ming Li, Yoichiroh Hosokawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b6ab8e8abfb4e96a05c223123c71a8b
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spelling	oai:doaj.org-article:5b6ab8e8abfb4e96a05c223123c71a8b2021-12-02T13:48:53ZHydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers10.1038/s41598-021-81190-y2045-2322https://doaj.org/article/5b6ab8e8abfb4e96a05c223123c71a8b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81190-yhttps://doaj.org/toc/2045-2322Abstract Microfluidic focusing of particles (both synthetic and biological), which enables precise control over the positions of particles in a tightly focused stream, is a prerequisite step for the downstream processing, such as detection, trapping and separation. In this study, we propose a novel hydrodynamic focusing method by taking advantage of open v-shaped microstructures on a glass substrate engraved by femtosecond pulse (fs) laser. The fs laser engraved microstructures were capable of focusing polystyrene particles and live cells in rectangular microchannels at relatively low Reynolds numbers (Re). Numerical simulations were performed to explain the mechanisms of particle focusing and experiments were carried out to investigate the effects of groove depth, groove number and flow rate on the performance of the groove-embedded microchannel for particle focusing. We found out that 10-µm polystyrene particles are directed toward the channel center under the effects of the groove-induced secondary flows in low-Re flows, e.g. Re < 1. Moreover, we achieved continuous focusing of live cells with different sizes ranging from 10 to 15 µm, i.e. human T-cell lymphoma Jurkat cells, rat adrenal pheochromocytoma PC12 cells and dog kidney MDCK cells. The glass grooves fabricated by fs laser are expected to be integrated with on-chip detection components, such as contact imaging and fluorescence lifetime-resolved imaging, for various biological and biomedical applications, where particle focusing at a relatively low flow rate is desirable.Tianlong ZhangMisuzu NamotoKazunori OkanoEri AkitaNorihiro TeranishiTao TangDian AnggrainiYansheng HaoYo TanakaDavid InglisYaxiaer YalikunMing LiYoichiroh HosokawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tianlong Zhang Misuzu Namoto Kazunori Okano Eri Akita Norihiro Teranishi Tao Tang Dian Anggraini Yansheng Hao Yo Tanaka David Inglis Yaxiaer Yalikun Ming Li Yoichiroh Hosokawa Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers
description	Abstract Microfluidic focusing of particles (both synthetic and biological), which enables precise control over the positions of particles in a tightly focused stream, is a prerequisite step for the downstream processing, such as detection, trapping and separation. In this study, we propose a novel hydrodynamic focusing method by taking advantage of open v-shaped microstructures on a glass substrate engraved by femtosecond pulse (fs) laser. The fs laser engraved microstructures were capable of focusing polystyrene particles and live cells in rectangular microchannels at relatively low Reynolds numbers (Re). Numerical simulations were performed to explain the mechanisms of particle focusing and experiments were carried out to investigate the effects of groove depth, groove number and flow rate on the performance of the groove-embedded microchannel for particle focusing. We found out that 10-µm polystyrene particles are directed toward the channel center under the effects of the groove-induced secondary flows in low-Re flows, e.g. Re < 1. Moreover, we achieved continuous focusing of live cells with different sizes ranging from 10 to 15 µm, i.e. human T-cell lymphoma Jurkat cells, rat adrenal pheochromocytoma PC12 cells and dog kidney MDCK cells. The glass grooves fabricated by fs laser are expected to be integrated with on-chip detection components, such as contact imaging and fluorescence lifetime-resolved imaging, for various biological and biomedical applications, where particle focusing at a relatively low flow rate is desirable.
format	article
author	Tianlong Zhang Misuzu Namoto Kazunori Okano Eri Akita Norihiro Teranishi Tao Tang Dian Anggraini Yansheng Hao Yo Tanaka David Inglis Yaxiaer Yalikun Ming Li Yoichiroh Hosokawa
author_facet	Tianlong Zhang Misuzu Namoto Kazunori Okano Eri Akita Norihiro Teranishi Tao Tang Dian Anggraini Yansheng Hao Yo Tanaka David Inglis Yaxiaer Yalikun Ming Li Yoichiroh Hosokawa
author_sort	Tianlong Zhang
title	Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers
title_short	Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers
title_full	Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers
title_fullStr	Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers
title_full_unstemmed	Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers
title_sort	hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low reynolds numbers
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5b6ab8e8abfb4e96a05c223123c71a8b
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Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers

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