Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).

Triacylglycerols (TAG) are the major molecules of energy storage in eukaryotes. TAG are packed in subcellular structures called oil bodies or lipid droplets. Oleosins (OLE) are the major proteins in plant oil bodies. Multiple isoforms of OLE are present in plants such as tung tree (Vernicia fordii),...

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Autores principales:	Heping Cao, Lin Zhang, Xiaofeng Tan, Hongxu Long, Jay M Shockey
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b6b0a1dad554e7ba970c6a533e4f7a5
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spelling	oai:doaj.org-article:5b6b0a1dad554e7ba970c6a533e4f7a52021-11-18T08:33:23ZIdentification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).1932-620310.1371/journal.pone.0088409https://doaj.org/article/5b6b0a1dad554e7ba970c6a533e4f7a52014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24516650/?tool=EBIhttps://doaj.org/toc/1932-6203Triacylglycerols (TAG) are the major molecules of energy storage in eukaryotes. TAG are packed in subcellular structures called oil bodies or lipid droplets. Oleosins (OLE) are the major proteins in plant oil bodies. Multiple isoforms of OLE are present in plants such as tung tree (Vernicia fordii), whose seeds are rich in novel TAG with a wide range of industrial applications. The objectives of this study were to identify OLE genes, classify OLE proteins and analyze OLE gene expression in tung trees. We identified five tung tree OLE genes coding for small hydrophobic proteins. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that the five tung OLE genes represented the five OLE subfamilies and all contained the "proline knot" motif (PX5SPX3P) shared among 65 OLE from 19 tree species, including the sequenced genomes of Prunus persica (peach), Populus trichocarpa (poplar), Ricinus communis (castor bean), Theobroma cacao (cacao) and Vitis vinifera (grapevine). Tung OLE1, OLE2 and OLE3 belong to the S type and OLE4 and OLE5 belong to the SM type of Arabidopsis OLE. TaqMan and SYBR Green qPCR methods were used to study the differential expression of OLE genes in tung tree tissues. Expression results demonstrated that 1) All five OLE genes were expressed in developing tung seeds, leaves and flowers; 2) OLE mRNA levels were much higher in seeds than leaves or flowers; 3) OLE1, OLE2 and OLE3 genes were expressed in tung seeds at much higher levels than OLE4 and OLE5 genes; 4) OLE mRNA levels rapidly increased during seed development; and 5) OLE gene expression was well-coordinated with tung oil accumulation in the seeds. These results suggest that tung OLE genes 1-3 probably play major roles in tung oil accumulation and/or oil body development. Therefore, they might be preferred targets for tung oil engineering in transgenic plants.Heping CaoLin ZhangXiaofeng TanHongxu LongJay M ShockeyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88409 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heping Cao Lin Zhang Xiaofeng Tan Hongxu Long Jay M Shockey Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).
description	Triacylglycerols (TAG) are the major molecules of energy storage in eukaryotes. TAG are packed in subcellular structures called oil bodies or lipid droplets. Oleosins (OLE) are the major proteins in plant oil bodies. Multiple isoforms of OLE are present in plants such as tung tree (Vernicia fordii), whose seeds are rich in novel TAG with a wide range of industrial applications. The objectives of this study were to identify OLE genes, classify OLE proteins and analyze OLE gene expression in tung trees. We identified five tung tree OLE genes coding for small hydrophobic proteins. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that the five tung OLE genes represented the five OLE subfamilies and all contained the "proline knot" motif (PX5SPX3P) shared among 65 OLE from 19 tree species, including the sequenced genomes of Prunus persica (peach), Populus trichocarpa (poplar), Ricinus communis (castor bean), Theobroma cacao (cacao) and Vitis vinifera (grapevine). Tung OLE1, OLE2 and OLE3 belong to the S type and OLE4 and OLE5 belong to the SM type of Arabidopsis OLE. TaqMan and SYBR Green qPCR methods were used to study the differential expression of OLE genes in tung tree tissues. Expression results demonstrated that 1) All five OLE genes were expressed in developing tung seeds, leaves and flowers; 2) OLE mRNA levels were much higher in seeds than leaves or flowers; 3) OLE1, OLE2 and OLE3 genes were expressed in tung seeds at much higher levels than OLE4 and OLE5 genes; 4) OLE mRNA levels rapidly increased during seed development; and 5) OLE gene expression was well-coordinated with tung oil accumulation in the seeds. These results suggest that tung OLE genes 1-3 probably play major roles in tung oil accumulation and/or oil body development. Therefore, they might be preferred targets for tung oil engineering in transgenic plants.
format	article
author	Heping Cao Lin Zhang Xiaofeng Tan Hongxu Long Jay M Shockey
author_facet	Heping Cao Lin Zhang Xiaofeng Tan Hongxu Long Jay M Shockey
author_sort	Heping Cao
title	Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).
title_short	Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).
title_full	Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).
title_fullStr	Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).
title_full_unstemmed	Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).
title_sort	identification, classification and differential expression of oleosin genes in tung tree (vernicia fordii).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/5b6b0a1dad554e7ba970c6a533e4f7a5
work_keys_str_mv	AT hepingcao identificationclassificationanddifferentialexpressionofoleosingenesintungtreeverniciafordii AT linzhang identificationclassificationanddifferentialexpressionofoleosingenesintungtreeverniciafordii AT xiaofengtan identificationclassificationanddifferentialexpressionofoleosingenesintungtreeverniciafordii AT hongxulong identificationclassificationanddifferentialexpressionofoleosingenesintungtreeverniciafordii AT jaymshockey identificationclassificationanddifferentialexpressionofoleosingenesintungtreeverniciafordii
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Identification, classification and differential expression of oleosin genes in tung tree (Vernicia fordii).

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