Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe

Abstract The development of a rapid, sensitive, and straightforward detection method of prostate‐specific antigen (PSA) is indispensable for the early diagnosis of prostate cancer (PCa). This work relates an electrochemical method using functionalized single‐stranded DNA aptamer to diagnose PCa and...

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Autores principales:	Zahra Aayanifard, Talieh Alebrahim, Mehrab Pourmadadi, Fatemeh Yazdian, Homayoon Soleimani Dinani, Hamid Rashedi, Meisam Omidi
Formato:	article
Lenguaje:	EN
Publicado:	Wiley-VCH 2021
Materias:	electrochemical aptasensor gold nanoparticle graphene oxide PSA detection TiO2/CQD Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5b713f3b43874d94a1211411ab904c5e
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spelling	oai:doaj.org-article:5b713f3b43874d94a1211411ab904c5e2021-11-09T05:07:05ZUltra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe1618-28631618-024010.1002/elsc.202000118https://doaj.org/article/5b713f3b43874d94a1211411ab904c5e2021-11-01T00:00:00Zhttps://doi.org/10.1002/elsc.202000118https://doaj.org/toc/1618-0240https://doaj.org/toc/1618-2863Abstract The development of a rapid, sensitive, and straightforward detection method of prostate‐specific antigen (PSA) is indispensable for the early diagnosis of prostate cancer (PCa). This work relates an electrochemical method using functionalized single‐stranded DNA aptamer to diagnose PCa and benign prostate hyperplasia. The sensing platform relies on PSA recognition by aptamer/Au/GO‐nanohybrid‐modified glassy carbon electrode. Besides ferrocyanide TiO2/carbon quantum dots (CQDs) probe is used to investigate the effect of nanoparticle‐containing electrolyte. Optimization of incubation time of aptamer/Au/GO‐nanohybrid and volume fraction of nafion were done using Design Expert 10 software reporting 42.4 h and 0.095% V/V, respectively. In ferrocyanide medium, PSA detection as low as 3, 2.96, and 0.85 ng mL−1 was achieved with a dynamic range from 0.5 to 7 ng ml−1, in accord with clinical values, using cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy, respectively. Moreover, this sensor exhibited conspicuous performance in TiO2/CQDs‐containing medium with different pH values of 5.4 and 8 to distinguish total PSA and free PSA, resulting in very low limit of detections, 0.028, and 0.007 ng ml−1, respectively. The results manifested the proposed system as a forthcoming sensor in a clinical and point of care analysis of PSA.Zahra AayanifardTalieh AlebrahimMehrab PourmadadiFatemeh YazdianHomayoon Soleimani DinaniHamid RashediMeisam OmidiWiley-VCHarticleelectrochemical aptasensorgold nanoparticlegraphene oxidePSA detectionTiO2/CQDBiotechnologyTP248.13-248.65ENEngineering in Life Sciences, Vol 21, Iss 11, Pp 739-752 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electrochemical aptasensor gold nanoparticle graphene oxide PSA detection TiO2/CQD Biotechnology TP248.13-248.65
spellingShingle	electrochemical aptasensor gold nanoparticle graphene oxide PSA detection TiO2/CQD Biotechnology TP248.13-248.65 Zahra Aayanifard Talieh Alebrahim Mehrab Pourmadadi Fatemeh Yazdian Homayoon Soleimani Dinani Hamid Rashedi Meisam Omidi Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe
description	Abstract The development of a rapid, sensitive, and straightforward detection method of prostate‐specific antigen (PSA) is indispensable for the early diagnosis of prostate cancer (PCa). This work relates an electrochemical method using functionalized single‐stranded DNA aptamer to diagnose PCa and benign prostate hyperplasia. The sensing platform relies on PSA recognition by aptamer/Au/GO‐nanohybrid‐modified glassy carbon electrode. Besides ferrocyanide TiO2/carbon quantum dots (CQDs) probe is used to investigate the effect of nanoparticle‐containing electrolyte. Optimization of incubation time of aptamer/Au/GO‐nanohybrid and volume fraction of nafion were done using Design Expert 10 software reporting 42.4 h and 0.095% V/V, respectively. In ferrocyanide medium, PSA detection as low as 3, 2.96, and 0.85 ng mL−1 was achieved with a dynamic range from 0.5 to 7 ng ml−1, in accord with clinical values, using cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy, respectively. Moreover, this sensor exhibited conspicuous performance in TiO2/CQDs‐containing medium with different pH values of 5.4 and 8 to distinguish total PSA and free PSA, resulting in very low limit of detections, 0.028, and 0.007 ng ml−1, respectively. The results manifested the proposed system as a forthcoming sensor in a clinical and point of care analysis of PSA.
format	article
author	Zahra Aayanifard Talieh Alebrahim Mehrab Pourmadadi Fatemeh Yazdian Homayoon Soleimani Dinani Hamid Rashedi Meisam Omidi
author_facet	Zahra Aayanifard Talieh Alebrahim Mehrab Pourmadadi Fatemeh Yazdian Homayoon Soleimani Dinani Hamid Rashedi Meisam Omidi
author_sort	Zahra Aayanifard
title	Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe
title_short	Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe
title_full	Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe
title_fullStr	Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe
title_full_unstemmed	Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe
title_sort	ultra ph‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on tio2/carbon quantum dots as a redox probe
publisher	Wiley-VCH
publishDate	2021
url	https://doaj.org/article/5b713f3b43874d94a1211411ab904c5e
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Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum dots as a redox probe

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