Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry

Abstract Macrophage lipid metabolism plays a pivotal role in innate and adaptive immune responses. Previous studies have shown that this process plays a role in infections and contributes to the pathogenesis of diabetes, atherosclerosis, and other immunometabolic diseases. M1 macrophages, or classic...

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Autores principales:	Cuiping Zhang, Yi Wang, Fang Wang, Zhenxin Wang, Yu Lu, Ying Xu, Ke Wang, Huali Shen, Pengyuan Yang, Shan Li, Xue Qin, Hongxiu Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b744be146374c9181f032635872c957
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spelling	oai:doaj.org-article:5b744be146374c9181f032635872c9572021-12-02T16:06:15ZQuantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry10.1038/s41598-017-00341-22045-2322https://doaj.org/article/5b744be146374c9181f032635872c9572017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00341-2https://doaj.org/toc/2045-2322Abstract Macrophage lipid metabolism plays a pivotal role in innate and adaptive immune responses. Previous studies have shown that this process plays a role in infections and contributes to the pathogenesis of diabetes, atherosclerosis, and other immunometabolic diseases. M1 macrophages, or classically activated macrophages, are key players in the defense against bacterial infections. M2 macrophages, or alternatively activated macrophages, are involved in anti-inflammatory responses. Using the multiple reaction monitoring method, we identified changes in lipid composition during the differentiation of human and murine macrophages. We detected over 300 lipid molecules in mammalian macrophages, and we observed a striking shift in the composition of glycerophospholipids (GLs) from saturated and monounsaturated to polyunsaturated during human macrophage polarization. Moreover, M2 macrophages showed a higher level of lysophospholipids (lysoGLs) than did M1 macrophages. The lysoPI species increased in human and mouse M2 macrophages, suggesting that they may be involved in M2 macrophage polarization and anti-inflammatory processes. Collectively, these results indicate that lipids may play a role in the pro- and anti-inflammatory activities of macrophages and may be markers of the macrophage activation state.Cuiping ZhangYi WangFang WangZhenxin WangYu LuYing XuKe WangHuali ShenPengyuan YangShan LiXue QinHongxiu YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cuiping Zhang Yi Wang Fang Wang Zhenxin Wang Yu Lu Ying Xu Ke Wang Huali Shen Pengyuan Yang Shan Li Xue Qin Hongxiu Yu Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry
description	Abstract Macrophage lipid metabolism plays a pivotal role in innate and adaptive immune responses. Previous studies have shown that this process plays a role in infections and contributes to the pathogenesis of diabetes, atherosclerosis, and other immunometabolic diseases. M1 macrophages, or classically activated macrophages, are key players in the defense against bacterial infections. M2 macrophages, or alternatively activated macrophages, are involved in anti-inflammatory responses. Using the multiple reaction monitoring method, we identified changes in lipid composition during the differentiation of human and murine macrophages. We detected over 300 lipid molecules in mammalian macrophages, and we observed a striking shift in the composition of glycerophospholipids (GLs) from saturated and monounsaturated to polyunsaturated during human macrophage polarization. Moreover, M2 macrophages showed a higher level of lysophospholipids (lysoGLs) than did M1 macrophages. The lysoPI species increased in human and mouse M2 macrophages, suggesting that they may be involved in M2 macrophage polarization and anti-inflammatory processes. Collectively, these results indicate that lipids may play a role in the pro- and anti-inflammatory activities of macrophages and may be markers of the macrophage activation state.
format	article
author	Cuiping Zhang Yi Wang Fang Wang Zhenxin Wang Yu Lu Ying Xu Ke Wang Huali Shen Pengyuan Yang Shan Li Xue Qin Hongxiu Yu
author_facet	Cuiping Zhang Yi Wang Fang Wang Zhenxin Wang Yu Lu Ying Xu Ke Wang Huali Shen Pengyuan Yang Shan Li Xue Qin Hongxiu Yu
author_sort	Cuiping Zhang
title	Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry
title_short	Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry
title_full	Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry
title_fullStr	Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry
title_full_unstemmed	Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry
title_sort	quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5b744be146374c9181f032635872c957
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Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry

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