TNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain

Lumbar disc herniation (LDH) with radicular pain is a common and complicated musculoskeletal disorder. Our previous study showed that LDH-induced methylglyoxal (MG) accumulation contributed to radicular pain. The underlying mechanisms through which MG accumulates are poorly understood. In the presen...

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Autores principales:	Xinsheng Zhang, Xiaogang Wang, Liang Gao, Bin Yang, Yahan Wang, Kerun Niu, Jiahui Lai, Shun Wan, Jianping Luo
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lumbar disc herniation methylglyoxal TNF-ɑ pain GLO1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/5b7a91135fd84cb29bc553c520571a84
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spelling	oai:doaj.org-article:5b7a91135fd84cb29bc553c520571a842021-11-30T12:37:16ZTNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain1662-515310.3389/fnbeh.2021.760547https://doaj.org/article/5b7a91135fd84cb29bc553c520571a842021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnbeh.2021.760547/fullhttps://doaj.org/toc/1662-5153Lumbar disc herniation (LDH) with radicular pain is a common and complicated musculoskeletal disorder. Our previous study showed that LDH-induced methylglyoxal (MG) accumulation contributed to radicular pain. The underlying mechanisms through which MG accumulates are poorly understood. In the present study, we found that both MG and tumor necrosis factor-alpha (TNF-ɑ) levels in the herniated disc of patients with radicular pain were significantly increased, and the activity of Glyoxalase 1 (GLO1), the rate-limiting enzyme that metabolizes MG, was decreased. In rats, the LDH model was mimicked by implantation of autologous nucleus pulposus (NP) to the left lumbar five spinal nerve root. The mechanical allodynia was observed in LDH rats. Besides, MG and TNF-ɑ levels were increased, and GLO1 activity was significantly decreased in the implanted NP. In cultured rat NP cells, stimulation with the inflammatory mediator TNF-ɑ reduced GLO1 activity and expression. These results suggested that TNF-ɑ-induced GLO1 activity decrease contributed to MG accumulation in the herniated disc of patients with radicular pain.Xinsheng ZhangXiaogang WangLiang GaoLiang GaoBin YangYahan WangKerun NiuJiahui LaiShun WanJianping LuoFrontiers Media S.A.articlelumbar disc herniationmethylglyoxalTNF-ɑpainGLO1Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Behavioral Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lumbar disc herniation methylglyoxal TNF-ɑ pain GLO1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	lumbar disc herniation methylglyoxal TNF-ɑ pain GLO1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Xinsheng Zhang Xiaogang Wang Liang Gao Liang Gao Bin Yang Yahan Wang Kerun Niu Jiahui Lai Shun Wan Jianping Luo TNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain
description	Lumbar disc herniation (LDH) with radicular pain is a common and complicated musculoskeletal disorder. Our previous study showed that LDH-induced methylglyoxal (MG) accumulation contributed to radicular pain. The underlying mechanisms through which MG accumulates are poorly understood. In the present study, we found that both MG and tumor necrosis factor-alpha (TNF-ɑ) levels in the herniated disc of patients with radicular pain were significantly increased, and the activity of Glyoxalase 1 (GLO1), the rate-limiting enzyme that metabolizes MG, was decreased. In rats, the LDH model was mimicked by implantation of autologous nucleus pulposus (NP) to the left lumbar five spinal nerve root. The mechanical allodynia was observed in LDH rats. Besides, MG and TNF-ɑ levels were increased, and GLO1 activity was significantly decreased in the implanted NP. In cultured rat NP cells, stimulation with the inflammatory mediator TNF-ɑ reduced GLO1 activity and expression. These results suggested that TNF-ɑ-induced GLO1 activity decrease contributed to MG accumulation in the herniated disc of patients with radicular pain.
format	article
author	Xinsheng Zhang Xiaogang Wang Liang Gao Liang Gao Bin Yang Yahan Wang Kerun Niu Jiahui Lai Shun Wan Jianping Luo
author_facet	Xinsheng Zhang Xiaogang Wang Liang Gao Liang Gao Bin Yang Yahan Wang Kerun Niu Jiahui Lai Shun Wan Jianping Luo
author_sort	Xinsheng Zhang
title	TNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain
title_short	TNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain
title_full	TNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain
title_fullStr	TNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain
title_full_unstemmed	TNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain
title_sort	tnf-ɑ induces methylglyoxal accumulation in lumbar herniated disc of patients with radicular pain
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5b7a91135fd84cb29bc553c520571a84
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TNF-ɑ Induces Methylglyoxal Accumulation in Lumbar Herniated Disc of Patients With Radicular Pain

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