dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.

Stem cells have the potential to maintain undifferentiated state and differentiate into specialized cell types. Despite numerous progress has been achieved in understanding stem cell self-renewal and differentiation, many fundamental questions remain unanswered. In this study, we identify dRTEL1, th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ying Yang, Ruiyan Kong, Feng Guang Goh, W Gregory Somers, Gary R Hime, Zhouhua Li, Yu Cai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/5b81154239cb44039f705354796331a1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5b81154239cb44039f705354796331a1
record_format	dspace
spelling	oai:doaj.org-article:5b81154239cb44039f705354796331a12021-12-02T20:03:31ZdRTEL1 is essential for the maintenance of Drosophila male germline stem cells.1553-73901553-740410.1371/journal.pgen.1009834https://doaj.org/article/5b81154239cb44039f705354796331a12021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009834https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Stem cells have the potential to maintain undifferentiated state and differentiate into specialized cell types. Despite numerous progress has been achieved in understanding stem cell self-renewal and differentiation, many fundamental questions remain unanswered. In this study, we identify dRTEL1, the Drosophila homolog of Regulator of Telomere Elongation Helicase 1, as a novel regulator of male germline stem cells (GSCs). Our genome-wide transcriptome analysis and ChIP-Seq results suggest that dRTEL1 affects a set of candidate genes required for GSC maintenance, likely independent of its role in DNA repair. Furthermore, dRTEL1 prevents DNA damage-induced checkpoint activation in GSCs. Finally, dRTEL1 functions to sustain Stat92E protein levels, the key player in GSC maintenance. Together, our findings reveal an intrinsic role of the DNA helicase dRTEL1 in maintaining male GSC and provide insight into the function of dRTEL1.Ying YangRuiyan KongFeng Guang GohW Gregory SomersGary R HimeZhouhua LiYu CaiPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 10, p e1009834 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Ying Yang Ruiyan Kong Feng Guang Goh W Gregory Somers Gary R Hime Zhouhua Li Yu Cai dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.
description	Stem cells have the potential to maintain undifferentiated state and differentiate into specialized cell types. Despite numerous progress has been achieved in understanding stem cell self-renewal and differentiation, many fundamental questions remain unanswered. In this study, we identify dRTEL1, the Drosophila homolog of Regulator of Telomere Elongation Helicase 1, as a novel regulator of male germline stem cells (GSCs). Our genome-wide transcriptome analysis and ChIP-Seq results suggest that dRTEL1 affects a set of candidate genes required for GSC maintenance, likely independent of its role in DNA repair. Furthermore, dRTEL1 prevents DNA damage-induced checkpoint activation in GSCs. Finally, dRTEL1 functions to sustain Stat92E protein levels, the key player in GSC maintenance. Together, our findings reveal an intrinsic role of the DNA helicase dRTEL1 in maintaining male GSC and provide insight into the function of dRTEL1.
format	article
author	Ying Yang Ruiyan Kong Feng Guang Goh W Gregory Somers Gary R Hime Zhouhua Li Yu Cai
author_facet	Ying Yang Ruiyan Kong Feng Guang Goh W Gregory Somers Gary R Hime Zhouhua Li Yu Cai
author_sort	Ying Yang
title	dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.
title_short	dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.
title_full	dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.
title_fullStr	dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.
title_full_unstemmed	dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.
title_sort	drtel1 is essential for the maintenance of drosophila male germline stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5b81154239cb44039f705354796331a1
work_keys_str_mv	AT yingyang drtel1isessentialforthemaintenanceofdrosophilamalegermlinestemcells AT ruiyankong drtel1isessentialforthemaintenanceofdrosophilamalegermlinestemcells AT fengguanggoh drtel1isessentialforthemaintenanceofdrosophilamalegermlinestemcells AT wgregorysomers drtel1isessentialforthemaintenanceofdrosophilamalegermlinestemcells AT garyrhime drtel1isessentialforthemaintenanceofdrosophilamalegermlinestemcells AT zhouhuali drtel1isessentialforthemaintenanceofdrosophilamalegermlinestemcells AT yucai drtel1isessentialforthemaintenanceofdrosophilamalegermlinestemcells
_version_	1718375661074120704

dRTEL1 is essential for the maintenance of Drosophila male germline stem cells.

Ejemplares similares