Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth

HN Lim1, NM Huang2, SS Lim3, I Harrison3, CH Chia41Centre for Ionics University of Malaya, Physics Department, Faculty of Science, University of Malaya, Kuala Lumpur, 2Low Dimensional Materials Research Centre, Physics Department, Faculty of Science, University of Malaya, Kuala Lumpur, 3School of Ch...

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Autores principales:	Lim HN, Huang NM, Lim SS, Harrison I, Chia CH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5b8175bfcb0d4bc0942629c3a43faff2
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spelling	oai:doaj.org-article:5b8175bfcb0d4bc0942629c3a43faff22021-12-02T05:55:47ZFabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth1176-91141178-2013https://doaj.org/article/5b8175bfcb0d4bc0942629c3a43faff22011-08-01T00:00:00Zhttp://www.dovepress.com/fabrication-and-characterization-of-graphene-hydrogel-via-hydrothermal-a8180https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013HN Lim1, NM Huang2, SS Lim3, I Harrison3, CH Chia41Centre for Ionics University of Malaya, Physics Department, Faculty of Science, University of Malaya, Kuala Lumpur, 2Low Dimensional Materials Research Centre, Physics Department, Faculty of Science, University of Malaya, Kuala Lumpur, 3School of Chemical and Environmental Engineering, Faculty of Engineering, The University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor, 4School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, MalaysiaBackground: Three-dimensional assembly of graphene hydrogel is rapidly attracting the interest of researchers because of its wide range of applications in energy storage, electronics, electrochemistry, and waste water treatment. Information on the use of graphene hydrogel for biological purposes is lacking, so we conducted a preliminary study to determine the suitability of graphene hydrogel as a substrate for cell growth, which could potentially be used as building blocks for biomolecules and tissue engineering applications.Methods: A three-dimensional structure of graphene hydrogel was prepared via a simple hydrothermal method using two-dimensional large-area graphene oxide nanosheets as a precursor.Results: The concentration and lateral size of the graphene oxide nanosheets influenced the structure of the hydrogel. With larger-area graphene oxide nanosheets, the graphene hydrogel could be formed at a lower concentration. X-ray diffraction patterns revealed that the oxide functional groups on the graphene oxide nanosheets were reduced after hydrothermal treatment. The three-dimensional graphene hydrogel matrix was used as a scaffold for proliferation of a MG63 cell line.Conclusion: Guided filopodia protrusions of MG63 on the hydrogel were observed on the third day of cell culture, demonstrating compatibility of the graphene hydrogel structure for bioapplications.Keywords: cell culture, graphene, hydrogel&nbsp;Lim HNHuang NMLim SSHarrison IChia CHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1817-1823 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lim HN Huang NM Lim SS Harrison I Chia CH Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth
description	HN Lim1, NM Huang2, SS Lim3, I Harrison3, CH Chia41Centre for Ionics University of Malaya, Physics Department, Faculty of Science, University of Malaya, Kuala Lumpur, 2Low Dimensional Materials Research Centre, Physics Department, Faculty of Science, University of Malaya, Kuala Lumpur, 3School of Chemical and Environmental Engineering, Faculty of Engineering, The University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor, 4School of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, MalaysiaBackground: Three-dimensional assembly of graphene hydrogel is rapidly attracting the interest of researchers because of its wide range of applications in energy storage, electronics, electrochemistry, and waste water treatment. Information on the use of graphene hydrogel for biological purposes is lacking, so we conducted a preliminary study to determine the suitability of graphene hydrogel as a substrate for cell growth, which could potentially be used as building blocks for biomolecules and tissue engineering applications.Methods: A three-dimensional structure of graphene hydrogel was prepared via a simple hydrothermal method using two-dimensional large-area graphene oxide nanosheets as a precursor.Results: The concentration and lateral size of the graphene oxide nanosheets influenced the structure of the hydrogel. With larger-area graphene oxide nanosheets, the graphene hydrogel could be formed at a lower concentration. X-ray diffraction patterns revealed that the oxide functional groups on the graphene oxide nanosheets were reduced after hydrothermal treatment. The three-dimensional graphene hydrogel matrix was used as a scaffold for proliferation of a MG63 cell line.Conclusion: Guided filopodia protrusions of MG63 on the hydrogel were observed on the third day of cell culture, demonstrating compatibility of the graphene hydrogel structure for bioapplications.Keywords: cell culture, graphene, hydrogel&nbsp;
format	article
author	Lim HN Huang NM Lim SS Harrison I Chia CH
author_facet	Lim HN Huang NM Lim SS Harrison I Chia CH
author_sort	Lim HN
title	Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth
title_short	Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth
title_full	Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth
title_fullStr	Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth
title_full_unstemmed	Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth
title_sort	fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/5b8175bfcb0d4bc0942629c3a43faff2
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_version_	1718400172870860800

Fabrication and characterization of graphene hydrogel via hydrothermal approach as a scaffold for preliminary study of cell growth

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