Efficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccine

Rift Valley Fever (RVF) is an acute infectious zoonotic arthropod-born viral disease, affecting many species of animals and it causes great economic losses in animal wealth and has a zoonotic implication. Eradication of mosquito and vaccination is an important and best method for preventing and cont...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Atwa, M.H, Maha Raafat Abd El-Fadeel, Heba A. Khafagy, Alaa A.El-Kholy, Ebtsam E.Z.Kotb, Marwa, F.Mahmoud
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2020
Materias:	elisa ni rvf saponin snt Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2020.85571 https://doaj.org/article/5b81fa78169c46d09169351c98effe43
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5b81fa78169c46d09169351c98effe43
record_format	dspace
spelling	oai:doaj.org-article:5b81fa78169c46d09169351c98effe432021-12-02T11:43:52ZEfficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccinehttps://dx.doi.org/10.21608/javs.2020.855711687-40722090-3308https://doaj.org/article/5b81fa78169c46d09169351c98effe432020-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_85571.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Rift Valley Fever (RVF) is an acute infectious zoonotic arthropod-born viral disease, affecting many species of animals and it causes great economic losses in animal wealth and has a zoonotic implication. Eradication of mosquito and vaccination is an important and best method for preventing and controlling the disease. This study was applied for the preparation and evaluation of Rift Valley Fever inactivated vaccine in a lyophilized form that is reconstituted at the time of inoculation using saponin that acts as an adjuvant. The prepared vaccine was proved to be sterile and safe. ED50 Potency test of the prepared vaccine in mice gave 0.0010 ED50/ML (permissible limit less than 0.02/ml). Sheep were vaccinated with 1ml S\C of the prepared lyophilized inactivated RVF vaccine and another group was vaccinated S\|C with 1ml of Aluminum hydroxide inactivated RVF vaccine. The immune response of different vaccinated sheep groups was evaluated using SNT and ELISA. The Prepared lyophilized inactivated RVF vaccine gave protective NI at the second-week post-vaccination (2.1 and 0.286 OD) , then reach to peak at the 4th week (3.28 and 0.343OD) then began to decline till ten months (1.8 and 0.241 OD), while the Aluminum hydroxide inactivated RVF vaccine gave protective NI at the second-week post-vaccination (1.7 and 0.277 OD) then reach to its NI peak at the 4th week (3.13 and 0.312 OD) then began to decline till ten months (1.73 and 0.228 OD). From the obtained results, it was found that the prepared lyophilized inactivated RVF vaccine was safe, potent and gave approximately similar immune response as aluminum hydroxide inactivated RVF vaccine but it is better as it reduces time and effort consuming during vaccine production.Atwa, M.HMaha Raafat Abd El-FadeelHeba A. KhafagyAlaa A.El-KholyEbtsam E.Z.KotbMarwa, F.MahmoudEgyptian Society for Animal ManagementarticleelisanirvfsaponinsntZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 5, Iss 2, Pp 33-39 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	elisa ni rvf saponin snt Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	elisa ni rvf saponin snt Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Atwa, M.H Maha Raafat Abd El-Fadeel Heba A. Khafagy Alaa A.El-Kholy Ebtsam E.Z.Kotb Marwa, F.Mahmoud Efficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccine
description	Rift Valley Fever (RVF) is an acute infectious zoonotic arthropod-born viral disease, affecting many species of animals and it causes great economic losses in animal wealth and has a zoonotic implication. Eradication of mosquito and vaccination is an important and best method for preventing and controlling the disease. This study was applied for the preparation and evaluation of Rift Valley Fever inactivated vaccine in a lyophilized form that is reconstituted at the time of inoculation using saponin that acts as an adjuvant. The prepared vaccine was proved to be sterile and safe. ED50 Potency test of the prepared vaccine in mice gave 0.0010 ED50/ML (permissible limit less than 0.02/ml). Sheep were vaccinated with 1ml S\C of the prepared lyophilized inactivated RVF vaccine and another group was vaccinated S\|C with 1ml of Aluminum hydroxide inactivated RVF vaccine. The immune response of different vaccinated sheep groups was evaluated using SNT and ELISA. The Prepared lyophilized inactivated RVF vaccine gave protective NI at the second-week post-vaccination (2.1 and 0.286 OD) , then reach to peak at the 4th week (3.28 and 0.343OD) then began to decline till ten months (1.8 and 0.241 OD), while the Aluminum hydroxide inactivated RVF vaccine gave protective NI at the second-week post-vaccination (1.7 and 0.277 OD) then reach to its NI peak at the 4th week (3.13 and 0.312 OD) then began to decline till ten months (1.73 and 0.228 OD). From the obtained results, it was found that the prepared lyophilized inactivated RVF vaccine was safe, potent and gave approximately similar immune response as aluminum hydroxide inactivated RVF vaccine but it is better as it reduces time and effort consuming during vaccine production.
format	article
author	Atwa, M.H Maha Raafat Abd El-Fadeel Heba A. Khafagy Alaa A.El-Kholy Ebtsam E.Z.Kotb Marwa, F.Mahmoud
author_facet	Atwa, M.H Maha Raafat Abd El-Fadeel Heba A. Khafagy Alaa A.El-Kholy Ebtsam E.Z.Kotb Marwa, F.Mahmoud
author_sort	Atwa, M.H
title	Efficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccine
title_short	Efficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccine
title_full	Efficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccine
title_fullStr	Efficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccine
title_full_unstemmed	Efficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccine
title_sort	efficacy of freeze-dried inactivated rift valley fever vaccine
publisher	Egyptian Society for Animal Management
publishDate	2020
url	https://dx.doi.org/10.21608/javs.2020.85571 https://doaj.org/article/5b81fa78169c46d09169351c98effe43
work_keys_str_mv	AT atwamh efficacyoffreezedriedinactivatedriftvalleyfevervaccine AT maharaafatabdelfadeel efficacyoffreezedriedinactivatedriftvalleyfevervaccine AT hebaakhafagy efficacyoffreezedriedinactivatedriftvalleyfevervaccine AT alaaaelkholy efficacyoffreezedriedinactivatedriftvalleyfevervaccine AT ebtsamezkotb efficacyoffreezedriedinactivatedriftvalleyfevervaccine AT marwafmahmoud efficacyoffreezedriedinactivatedriftvalleyfevervaccine
_version_	1718395343310159872

Efficacy Of Freeze-Dried Inactivated Rift Valley Fever Vaccine

Ejemplares similares