APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING

The article deals with applications of six-color flow analysis for studying the immune state parameters by means of flow cytometry. Whole blood from healthy donors was stained with combination of monoclonal antibodies, i.e., HLA-DR-FITC, CD16+56-PE, CD4-ECD, CD19-ECD, CD8-PC5.5, CD3-PC7, CD45-APC (B...

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Autores principales:	I. V. Kudryavtsev, A. I. Subbotovskaya
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2015
Materias:	flow cytometry multicolor analysis immunophenotyping Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/5b894215711745bd80ecdec4d39b4ad3
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id	oai:doaj.org-article:5b894215711745bd80ecdec4d39b4ad3
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spelling	oai:doaj.org-article:5b894215711745bd80ecdec4d39b4ad32021-11-18T08:03:44ZAPPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING1563-06252313-741X10.15789/1563-0625-2015-1-19-26https://doaj.org/article/5b894215711745bd80ecdec4d39b4ad32015-03-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/803https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe article deals with applications of six-color flow analysis for studying the immune state parameters by means of flow cytometry. Whole blood from healthy donors was stained with combination of monoclonal antibodies, i.e., HLA-DR-FITC, CD16+56-PE, CD4-ECD, CD19-ECD, CD8-PC5.5, CD3-PC7, CD45-APC (Beckman Coulter, USA) using a “no-wash” technology. To adjust the photomultiplier (PMT) voltage, we used the tubes with each of the tested monoclonal antibodies, PMT voltage was considered optimal when the negative population was located in the middle of the first decade at the logarithmic scale. The compensatory adjustment was performed in automatic mode, using Navios software (Beckman Coulter, USA). We discuss an optimal gating strategy in order to assess the populations of interest: total T cells (CD3+CD19-), T helper cells (CD3+CD4+), cytotoxic T lymphocytes (CD3+CD8+), B cells (CD3-CD19+), NK cells (CD3-CD16+CD56+), NKT cells (CD3+CD16+CD56+), activated T lymphocytes (CD3+HLA-DR+).I. V. KudryavtsevA. I. SubbotovskayaSPb RAACIarticleflow cytometrymulticolor analysisimmunophenotypingImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 17, Iss 1, Pp 19-26 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	flow cytometry multicolor analysis immunophenotyping Immunologic diseases. Allergy RC581-607
spellingShingle	flow cytometry multicolor analysis immunophenotyping Immunologic diseases. Allergy RC581-607 I. V. Kudryavtsev A. I. Subbotovskaya APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING
description	The article deals with applications of six-color flow analysis for studying the immune state parameters by means of flow cytometry. Whole blood from healthy donors was stained with combination of monoclonal antibodies, i.e., HLA-DR-FITC, CD16+56-PE, CD4-ECD, CD19-ECD, CD8-PC5.5, CD3-PC7, CD45-APC (Beckman Coulter, USA) using a “no-wash” technology. To adjust the photomultiplier (PMT) voltage, we used the tubes with each of the tested monoclonal antibodies, PMT voltage was considered optimal when the negative population was located in the middle of the first decade at the logarithmic scale. The compensatory adjustment was performed in automatic mode, using Navios software (Beckman Coulter, USA). We discuss an optimal gating strategy in order to assess the populations of interest: total T cells (CD3+CD19-), T helper cells (CD3+CD4+), cytotoxic T lymphocytes (CD3+CD8+), B cells (CD3-CD19+), NK cells (CD3-CD16+CD56+), NKT cells (CD3+CD16+CD56+), activated T lymphocytes (CD3+HLA-DR+).
format	article
author	I. V. Kudryavtsev A. I. Subbotovskaya
author_facet	I. V. Kudryavtsev A. I. Subbotovskaya
author_sort	I. V. Kudryavtsev
title	APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING
title_short	APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING
title_full	APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING
title_fullStr	APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING
title_full_unstemmed	APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING
title_sort	application of six-color flow cytometric analysis for immune profile monitoring
publisher	SPb RAACI
publishDate	2015
url	https://doaj.org/article/5b894215711745bd80ecdec4d39b4ad3
work_keys_str_mv	AT ivkudryavtsev applicationofsixcolorflowcytometricanalysisforimmuneprofilemonitoring AT aisubbotovskaya applicationofsixcolorflowcytometricanalysisforimmuneprofilemonitoring
_version_	1718422419017826304

APPLICATION OF SIX-COLOR FLOW CYTOMETRIC ANALYSIS FOR IMMUNE PROFILE MONITORING

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