Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar

Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typ...

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Autores principales:	Mohammad R. Hasan, Mahesh K.R. Kalikiri, Faheem Mirza, Sathyavathi Sundararaju, Anju Sharma, Thabisile Xaba, Stephan Lorenz, Hiam Chemaitelly, Reham A. El-Kahlout, Kin Ming Tsui, Hadi M. Yassine, Peter V. Coyle, Abdullatif Al Khal, Roberto Bertollini, Mohamed H. Al Thani, Laith J. Abu-Raddad, Patrick Tang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	COVID-19 SARS-CoV-2 Variants of concern (VOC) Genotyping Epidemiology Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/5b92bed673c34a429f3266981bf579a9
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spelling	oai:doaj.org-article:5b92bed673c34a429f3266981bf579a92021-11-30T04:14:05ZReal-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar1201-971210.1016/j.ijid.2021.09.006https://doaj.org/article/5b92bed673c34a429f3266981bf579a92021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1201971221007098https://doaj.org/toc/1201-9712Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.Mohammad R. HasanMahesh K.R. KalikiriFaheem MirzaSathyavathi SundararajuAnju SharmaThabisile XabaStephan LorenzHiam ChemaitellyReham A. El-KahloutKin Ming TsuiHadi M. YassinePeter V. CoyleAbdullatif Al KhalRoberto BertolliniMohamed H. Al ThaniLaith J. Abu-RaddadPatrick TangElsevierarticleCOVID-19SARS-CoV-2Variants of concern (VOC)GenotypingEpidemiologyInfectious and parasitic diseasesRC109-216ENInternational Journal of Infectious Diseases, Vol 112, Iss , Pp 52-54 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 SARS-CoV-2 Variants of concern (VOC) Genotyping Epidemiology Infectious and parasitic diseases RC109-216
spellingShingle	COVID-19 SARS-CoV-2 Variants of concern (VOC) Genotyping Epidemiology Infectious and parasitic diseases RC109-216 Mohammad R. Hasan Mahesh K.R. Kalikiri Faheem Mirza Sathyavathi Sundararaju Anju Sharma Thabisile Xaba Stephan Lorenz Hiam Chemaitelly Reham A. El-Kahlout Kin Ming Tsui Hadi M. Yassine Peter V. Coyle Abdullatif Al Khal Roberto Bertollini Mohamed H. Al Thani Laith J. Abu-Raddad Patrick Tang Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar
description	Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.
format	article
author	Mohammad R. Hasan Mahesh K.R. Kalikiri Faheem Mirza Sathyavathi Sundararaju Anju Sharma Thabisile Xaba Stephan Lorenz Hiam Chemaitelly Reham A. El-Kahlout Kin Ming Tsui Hadi M. Yassine Peter V. Coyle Abdullatif Al Khal Roberto Bertollini Mohamed H. Al Thani Laith J. Abu-Raddad Patrick Tang
author_facet	Mohammad R. Hasan Mahesh K.R. Kalikiri Faheem Mirza Sathyavathi Sundararaju Anju Sharma Thabisile Xaba Stephan Lorenz Hiam Chemaitelly Reham A. El-Kahlout Kin Ming Tsui Hadi M. Yassine Peter V. Coyle Abdullatif Al Khal Roberto Bertollini Mohamed H. Al Thani Laith J. Abu-Raddad Patrick Tang
author_sort	Mohammad R. Hasan
title	Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar
title_short	Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar
title_full	Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar
title_fullStr	Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar
title_full_unstemmed	Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar
title_sort	real-time sars-cov-2 genotyping by high-throughput multiplex pcr reveals the epidemiology of the variants of concern in qatar
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5b92bed673c34a429f3266981bf579a9
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Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar

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