Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis

Abstract Perception of environmental and habitat cues is of significance for insect survival and reproduction. Odor detection in insects is mediated by a number of proteins in antennae such as odorant receptors (ORs), ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins...

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Autores principales:	Shiyong Yang, Depan Cao, Guirong Wang, Yang Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b9603615229423a9711850da732f71e
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spelling	oai:doaj.org-article:5b9603615229423a9711850da732f71e2021-12-02T15:05:05ZIdentification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis10.1038/s41598-017-11646-72045-2322https://doaj.org/article/5b9603615229423a9711850da732f71e2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-11646-7https://doaj.org/toc/2045-2322Abstract Perception of environmental and habitat cues is of significance for insect survival and reproduction. Odor detection in insects is mediated by a number of proteins in antennae such as odorant receptors (ORs), ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins (CSPs), sensory neuron membrane proteins (SNMPs) and odorant degrading enzymes. In this study, we sequenced and assembled the adult male and female antennal transcriptomes of a destructive agricultural pest, the diamondback moth Plutella xyllostella. In these transcriptomes, we identified transcripts belonging to 6 chemoreception gene families related to ordor detection, including 54 ORs, 16 IRs, 7 gustatory receptors (GRs), 15 CSPs, 24 OBPs and 2 SNMPs. Semi-quantitative reverse transcription PCR analysis of expression patterns indicated that some of these ORs and IRs have clear sex-biased and tissue-specific expression patterns. Our results lay the foundation for future characterization of the functions of these P. xyllostella chemosensory receptors at the molecular level and development of novel semiochemicals for integrated control of this agricultural pest.Shiyong YangDepan CaoGuirong WangYang LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shiyong Yang Depan Cao Guirong Wang Yang Liu Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis
description	Abstract Perception of environmental and habitat cues is of significance for insect survival and reproduction. Odor detection in insects is mediated by a number of proteins in antennae such as odorant receptors (ORs), ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins (CSPs), sensory neuron membrane proteins (SNMPs) and odorant degrading enzymes. In this study, we sequenced and assembled the adult male and female antennal transcriptomes of a destructive agricultural pest, the diamondback moth Plutella xyllostella. In these transcriptomes, we identified transcripts belonging to 6 chemoreception gene families related to ordor detection, including 54 ORs, 16 IRs, 7 gustatory receptors (GRs), 15 CSPs, 24 OBPs and 2 SNMPs. Semi-quantitative reverse transcription PCR analysis of expression patterns indicated that some of these ORs and IRs have clear sex-biased and tissue-specific expression patterns. Our results lay the foundation for future characterization of the functions of these P. xyllostella chemosensory receptors at the molecular level and development of novel semiochemicals for integrated control of this agricultural pest.
format	article
author	Shiyong Yang Depan Cao Guirong Wang Yang Liu
author_facet	Shiyong Yang Depan Cao Guirong Wang Yang Liu
author_sort	Shiyong Yang
title	Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis
title_short	Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis
title_full	Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis
title_fullStr	Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis
title_full_unstemmed	Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis
title_sort	identification of genes involved in chemoreception in plutella xyllostella by antennal transcriptome analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5b9603615229423a9711850da732f71e
work_keys_str_mv	AT shiyongyang identificationofgenesinvolvedinchemoreceptioninplutellaxyllostellabyantennaltranscriptomeanalysis AT depancao identificationofgenesinvolvedinchemoreceptioninplutellaxyllostellabyantennaltranscriptomeanalysis AT guirongwang identificationofgenesinvolvedinchemoreceptioninplutellaxyllostellabyantennaltranscriptomeanalysis AT yangliu identificationofgenesinvolvedinchemoreceptioninplutellaxyllostellabyantennaltranscriptomeanalysis
_version_	1718388957371170816

Identification of Genes Involved in Chemoreception in Plutella xyllostella by Antennal Transcriptome Analysis

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