Importance of the 5′ regulatory region to bacterial synthetic biology applications

Summary The field of synthetic biology is evolving at a fast pace. It is advancing beyond single‐gene alterations in single hosts to the logical design of complex circuits and the development of integrated synthetic genomes. Recent breakthroughs in deep learning, which is increasingly used in de nov...

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Autores principales:	Lisa Tietze, Rahmi Lale
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/5b96eca0a60a45d48fafc9c9edc499d6
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spelling	oai:doaj.org-article:5b96eca0a60a45d48fafc9c9edc499d62021-11-18T15:39:52ZImportance of the 5′ regulatory region to bacterial synthetic biology applications1751-791510.1111/1751-7915.13868https://doaj.org/article/5b96eca0a60a45d48fafc9c9edc499d62021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13868https://doaj.org/toc/1751-7915Summary The field of synthetic biology is evolving at a fast pace. It is advancing beyond single‐gene alterations in single hosts to the logical design of complex circuits and the development of integrated synthetic genomes. Recent breakthroughs in deep learning, which is increasingly used in de novo assembly of DNA components with predictable effects, are also aiding the discipline. Despite advances in computing, the field is still reliant on the availability of pre‐characterized DNA parts, whether natural or synthetic, to regulate gene expression in bacteria and make valuable compounds. In this review, we discuss the different bacterial synthetic biology methodologies employed in the creation of 5′ regulatory regions – promoters, untranslated regions and 5′‐end of coding sequences. We summarize methodologies and discuss their significance for each of the functional DNA components, and highlight the key advances made in bacterial engineering by concentrating on their flaws and strengths. We end the review by outlining the issues that the discipline may face in the near future.Lisa TietzeRahmi LaleWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2291-2315 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Lisa Tietze Rahmi Lale Importance of the 5′ regulatory region to bacterial synthetic biology applications
description	Summary The field of synthetic biology is evolving at a fast pace. It is advancing beyond single‐gene alterations in single hosts to the logical design of complex circuits and the development of integrated synthetic genomes. Recent breakthroughs in deep learning, which is increasingly used in de novo assembly of DNA components with predictable effects, are also aiding the discipline. Despite advances in computing, the field is still reliant on the availability of pre‐characterized DNA parts, whether natural or synthetic, to regulate gene expression in bacteria and make valuable compounds. In this review, we discuss the different bacterial synthetic biology methodologies employed in the creation of 5′ regulatory regions – promoters, untranslated regions and 5′‐end of coding sequences. We summarize methodologies and discuss their significance for each of the functional DNA components, and highlight the key advances made in bacterial engineering by concentrating on their flaws and strengths. We end the review by outlining the issues that the discipline may face in the near future.
format	article
author	Lisa Tietze Rahmi Lale
author_facet	Lisa Tietze Rahmi Lale
author_sort	Lisa Tietze
title	Importance of the 5′ regulatory region to bacterial synthetic biology applications
title_short	Importance of the 5′ regulatory region to bacterial synthetic biology applications
title_full	Importance of the 5′ regulatory region to bacterial synthetic biology applications
title_fullStr	Importance of the 5′ regulatory region to bacterial synthetic biology applications
title_full_unstemmed	Importance of the 5′ regulatory region to bacterial synthetic biology applications
title_sort	importance of the 5′ regulatory region to bacterial synthetic biology applications
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/5b96eca0a60a45d48fafc9c9edc499d6
work_keys_str_mv	AT lisatietze importanceofthe5regulatoryregiontobacterialsyntheticbiologyapplications AT rahmilale importanceofthe5regulatoryregiontobacterialsyntheticbiologyapplications
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Importance of the 5′ regulatory region to bacterial synthetic biology applications

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