High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells

Abstract The rate of the remodeling of the arterialized saphenous vein conduit limits the outcomes of coronary artery bypass graft surgery (CABG), which may be influenced by endothelial dysfunction. We tested the hypothesis that high stretch (HS) induces human saphenous vein endothelial cell (hSVEC)...

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Autores principales:	T. Girão-Silva, M. H. Fonseca-Alaniz, J. C. Ribeiro-Silva, J. Lee, N. P. Patil, L. A. Dallan, A. B. Baker, M. C. Harmsen, J. E. Krieger, A. A. Miyakawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b99600276664669bd72b959b27b2ca1
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spelling	oai:doaj.org-article:5b99600276664669bd72b959b27b2ca12021-12-02T16:32:13ZHigh stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells10.1038/s41598-021-93081-32045-2322https://doaj.org/article/5b99600276664669bd72b959b27b2ca12021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93081-3https://doaj.org/toc/2045-2322Abstract The rate of the remodeling of the arterialized saphenous vein conduit limits the outcomes of coronary artery bypass graft surgery (CABG), which may be influenced by endothelial dysfunction. We tested the hypothesis that high stretch (HS) induces human saphenous vein endothelial cell (hSVEC) dysfunction and examined candidate underlying mechanisms. Our results showed that in vitro HS reduces NO bioavailability, increases inflammatory adhesion molecule expression (E-selectin and VCAM1) and THP-1 cell adhesion. HS decreases F-actin in hSVECs, but not in human arterial endothelial cells, and is accompanied by G-actin and cofilin’s nuclear shuttling and increased reactive oxidative species (ROS). Pre-treatment with the broad-acting antioxidant N-acetylcysteine (NAC) supported this observation and diminished stretch-induced actin remodeling and inflammatory adhesive molecule expression. Altogether, we provide evidence that increased oxidative stress and actin cytoskeleton remodeling play a role in HS-induced saphenous vein endothelial cell dysfunction, which may contribute to predisposing saphenous vein graft to failure.T. Girão-SilvaM. H. Fonseca-AlanizJ. C. Ribeiro-SilvaJ. LeeN. P. PatilL. A. DallanA. B. BakerM. C. HarmsenJ. E. KriegerA. A. MiyakawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q T. Girão-Silva M. H. Fonseca-Alaniz J. C. Ribeiro-Silva J. Lee N. P. Patil L. A. Dallan A. B. Baker M. C. Harmsen J. E. Krieger A. A. Miyakawa High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells
description	Abstract The rate of the remodeling of the arterialized saphenous vein conduit limits the outcomes of coronary artery bypass graft surgery (CABG), which may be influenced by endothelial dysfunction. We tested the hypothesis that high stretch (HS) induces human saphenous vein endothelial cell (hSVEC) dysfunction and examined candidate underlying mechanisms. Our results showed that in vitro HS reduces NO bioavailability, increases inflammatory adhesion molecule expression (E-selectin and VCAM1) and THP-1 cell adhesion. HS decreases F-actin in hSVECs, but not in human arterial endothelial cells, and is accompanied by G-actin and cofilin’s nuclear shuttling and increased reactive oxidative species (ROS). Pre-treatment with the broad-acting antioxidant N-acetylcysteine (NAC) supported this observation and diminished stretch-induced actin remodeling and inflammatory adhesive molecule expression. Altogether, we provide evidence that increased oxidative stress and actin cytoskeleton remodeling play a role in HS-induced saphenous vein endothelial cell dysfunction, which may contribute to predisposing saphenous vein graft to failure.
format	article
author	T. Girão-Silva M. H. Fonseca-Alaniz J. C. Ribeiro-Silva J. Lee N. P. Patil L. A. Dallan A. B. Baker M. C. Harmsen J. E. Krieger A. A. Miyakawa
author_facet	T. Girão-Silva M. H. Fonseca-Alaniz J. C. Ribeiro-Silva J. Lee N. P. Patil L. A. Dallan A. B. Baker M. C. Harmsen J. E. Krieger A. A. Miyakawa
author_sort	T. Girão-Silva
title	High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells
title_short	High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells
title_full	High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells
title_fullStr	High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells
title_full_unstemmed	High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells
title_sort	high stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5b99600276664669bd72b959b27b2ca1
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High stretch induces endothelial dysfunction accompanied by oxidative stress and actin remodeling in human saphenous vein endothelial cells

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