Evaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.

Chickens were immunized subcutaneously with an Eimeria recombinant profilin protein plus Montanide™ ISA 70 VG (ISA 70) or Montanide™ ISA 71 VG (ISA 71) water-in-oil adjuvants, or with profilin alone, and comparative RNA microarray hybridizations were performed to ascertain global transcriptome chang...

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Autores principales:	Seung I Jang, Duk Kyung Kim, Hyun S Lillehoj, Sung Hyen Lee, Kyung Woo Lee, François Bertrand, Laurent Dupuis, Sébastien Deville, Juliette Ben Arous, Erik P Lillehoj
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b9b39f3b094455b8f1ba517767528bd
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spelling	oai:doaj.org-article:5b9b39f3b094455b8f1ba517767528bd2021-11-18T07:50:13ZEvaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.1932-620310.1371/journal.pone.0059786https://doaj.org/article/5b9b39f3b094455b8f1ba517767528bd2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593150/?tool=EBIhttps://doaj.org/toc/1932-6203Chickens were immunized subcutaneously with an Eimeria recombinant profilin protein plus Montanide™ ISA 70 VG (ISA 70) or Montanide™ ISA 71 VG (ISA 71) water-in-oil adjuvants, or with profilin alone, and comparative RNA microarray hybridizations were performed to ascertain global transcriptome changes induced by profilin/ISA 70 vs. profilin alone and by profilin/ISA 71 vs. profilin alone. While immunization with profilin/ISA 70 vs. profilin alone altered the levels of more total transcripts compared with profilin/ISA 71 vs. profilin alone (509 vs. 296), the latter was associated with a greater number of unique biological functions, and a larger number of genes within these functions, compared with the former. Further, canonical pathway analysis identified 10 pathways that were associated with genes encoding the altered transcripts in animals immunized with profilin/ISA 71 vs. profilin alone, compared with only 2 pathways in profilin/ISA 70 vs. profilin alone. Therefore, ISA 71 was selected as a candidate adjuvant in conjunction with profilin vaccination for in vivo disease protection studies. Vaccination with profilin/ISA 71 was associated with greater body weight gain following E. acervulina infection, and decreased parasite fecal shedding after E. maxima infection, compared with profilin alone. Anti-profilin antibody levels were higher in sera of E. maxima- and E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. Finally, the levels of transcripts encoding interferon-γ, interleukin (IL)-2, IL-10, and IL-17A were increased in intestinal lymphocytes from E. acervulina-, E. maxima-, and/or E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. None of these effects were seen in chickens injected with ISA 71 alone indicating that the adjuvant was not conferring non-specific immune stimulation. These results suggest that profilin plus ISA 71 augments protective immunity against selective Eimeria species in chickens.Seung I JangDuk Kyung KimHyun S LillehojSung Hyen LeeKyung Woo LeeFrançois BertrandLaurent DupuisSébastien DevilleJuliette Ben ArousErik P LillehojPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e59786 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seung I Jang Duk Kyung Kim Hyun S Lillehoj Sung Hyen Lee Kyung Woo Lee François Bertrand Laurent Dupuis Sébastien Deville Juliette Ben Arous Erik P Lillehoj Evaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.
description	Chickens were immunized subcutaneously with an Eimeria recombinant profilin protein plus Montanide™ ISA 70 VG (ISA 70) or Montanide™ ISA 71 VG (ISA 71) water-in-oil adjuvants, or with profilin alone, and comparative RNA microarray hybridizations were performed to ascertain global transcriptome changes induced by profilin/ISA 70 vs. profilin alone and by profilin/ISA 71 vs. profilin alone. While immunization with profilin/ISA 70 vs. profilin alone altered the levels of more total transcripts compared with profilin/ISA 71 vs. profilin alone (509 vs. 296), the latter was associated with a greater number of unique biological functions, and a larger number of genes within these functions, compared with the former. Further, canonical pathway analysis identified 10 pathways that were associated with genes encoding the altered transcripts in animals immunized with profilin/ISA 71 vs. profilin alone, compared with only 2 pathways in profilin/ISA 70 vs. profilin alone. Therefore, ISA 71 was selected as a candidate adjuvant in conjunction with profilin vaccination for in vivo disease protection studies. Vaccination with profilin/ISA 71 was associated with greater body weight gain following E. acervulina infection, and decreased parasite fecal shedding after E. maxima infection, compared with profilin alone. Anti-profilin antibody levels were higher in sera of E. maxima- and E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. Finally, the levels of transcripts encoding interferon-γ, interleukin (IL)-2, IL-10, and IL-17A were increased in intestinal lymphocytes from E. acervulina-, E. maxima-, and/or E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. None of these effects were seen in chickens injected with ISA 71 alone indicating that the adjuvant was not conferring non-specific immune stimulation. These results suggest that profilin plus ISA 71 augments protective immunity against selective Eimeria species in chickens.
format	article
author	Seung I Jang Duk Kyung Kim Hyun S Lillehoj Sung Hyen Lee Kyung Woo Lee François Bertrand Laurent Dupuis Sébastien Deville Juliette Ben Arous Erik P Lillehoj
author_facet	Seung I Jang Duk Kyung Kim Hyun S Lillehoj Sung Hyen Lee Kyung Woo Lee François Bertrand Laurent Dupuis Sébastien Deville Juliette Ben Arous Erik P Lillehoj
author_sort	Seung I Jang
title	Evaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.
title_short	Evaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.
title_full	Evaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.
title_fullStr	Evaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.
title_full_unstemmed	Evaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.
title_sort	evaluation of montanide™ isa 71 vg adjuvant during profilin vaccination against experimental coccidiosis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5b9b39f3b094455b8f1ba517767528bd
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Evaluation of Montanide™ ISA 71 VG adjuvant during profilin vaccination against experimental coccidiosis.

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