Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins

Abstract Clostridium difficile is the most important enteropathogen involved in gut nosocomial post-antibiotic infections. The emergence of hypervirulent strains has contributed to increased mortality and morbidity of CDI. The C. difficile toxins contribute directly to CDI-associated lesions of the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jameel Batah, Hussein Kobeissy, Phuong Trang Bui Pham, Cécile Denève-Larrazet, Sarah Kuehne, Anne Collignon, Claire Janoir-Jouveshomme, Jean-Christophe Marvaud, Imad Kansau
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5b9cae09e7414f9d900cc05bf5041ee6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5b9cae09e7414f9d900cc05bf5041ee6
record_format	dspace
spelling	oai:doaj.org-article:5b9cae09e7414f9d900cc05bf5041ee62021-12-02T12:30:45ZClostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins10.1038/s41598-017-03621-z2045-2322https://doaj.org/article/5b9cae09e7414f9d900cc05bf5041ee62017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03621-zhttps://doaj.org/toc/2045-2322Abstract Clostridium difficile is the most important enteropathogen involved in gut nosocomial post-antibiotic infections. The emergence of hypervirulent strains has contributed to increased mortality and morbidity of CDI. The C. difficile toxins contribute directly to CDI-associated lesions of the gut, but other bacterial factors are needed for the bacteria to adhere and colonize the intestinal epithelium. The C. difficile flagella, which confer motility and chemotaxis for successful intestinal colonization, could play an additional role in bacterial pathogenesis by contributing to the inflammatory response of the host and mucosal injury. Indeed, by activating the TLR5, flagella can elicit activation of the MAPK and NF-κB cascades of cell signaling, leading to the secretion of pro-inflammatory cytokines. In the current study, we demonstrate, by using an animal model of CDI, a synergic effect of flagella and toxins in eliciting an inflammatory mucosal response. In this model, the absence of flagella dramatically decreases the degree of mucosal inflammation in mice and the sole presence of toxins without flagella was not enough to elicit epithelial lesions. These results highlight the important role of C. difficile flagella in eliciting mucosal lesions as long as the toxins exert their action on the epithelium.Jameel BatahHussein KobeissyPhuong Trang Bui PhamCécile Denève-LarrazetSarah KuehneAnne CollignonClaire Janoir-JouveshommeJean-Christophe MarvaudImad KansauNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jameel Batah Hussein Kobeissy Phuong Trang Bui Pham Cécile Denève-Larrazet Sarah Kuehne Anne Collignon Claire Janoir-Jouveshomme Jean-Christophe Marvaud Imad Kansau Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins
description	Abstract Clostridium difficile is the most important enteropathogen involved in gut nosocomial post-antibiotic infections. The emergence of hypervirulent strains has contributed to increased mortality and morbidity of CDI. The C. difficile toxins contribute directly to CDI-associated lesions of the gut, but other bacterial factors are needed for the bacteria to adhere and colonize the intestinal epithelium. The C. difficile flagella, which confer motility and chemotaxis for successful intestinal colonization, could play an additional role in bacterial pathogenesis by contributing to the inflammatory response of the host and mucosal injury. Indeed, by activating the TLR5, flagella can elicit activation of the MAPK and NF-κB cascades of cell signaling, leading to the secretion of pro-inflammatory cytokines. In the current study, we demonstrate, by using an animal model of CDI, a synergic effect of flagella and toxins in eliciting an inflammatory mucosal response. In this model, the absence of flagella dramatically decreases the degree of mucosal inflammation in mice and the sole presence of toxins without flagella was not enough to elicit epithelial lesions. These results highlight the important role of C. difficile flagella in eliciting mucosal lesions as long as the toxins exert their action on the epithelium.
format	article
author	Jameel Batah Hussein Kobeissy Phuong Trang Bui Pham Cécile Denève-Larrazet Sarah Kuehne Anne Collignon Claire Janoir-Jouveshomme Jean-Christophe Marvaud Imad Kansau
author_facet	Jameel Batah Hussein Kobeissy Phuong Trang Bui Pham Cécile Denève-Larrazet Sarah Kuehne Anne Collignon Claire Janoir-Jouveshomme Jean-Christophe Marvaud Imad Kansau
author_sort	Jameel Batah
title	Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins
title_short	Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins
title_full	Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins
title_fullStr	Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins
title_full_unstemmed	Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins
title_sort	clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5b9cae09e7414f9d900cc05bf5041ee6
work_keys_str_mv	AT jameelbatah clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins AT husseinkobeissy clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins AT phuongtrangbuipham clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins AT ceciledenevelarrazet clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins AT sarahkuehne clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins AT annecollignon clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins AT clairejanoirjouveshomme clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins AT jeanchristophemarvaud clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins AT imadkansau clostridiumdifficileflagellainduceaproinflammatoryresponseinintestinalepitheliumofmiceincooperationwithtoxins
_version_	1718394337522352128

Clostridium difficile flagella induce a pro-inflammatory response in intestinal epithelium of mice in cooperation with toxins

Ejemplares similares