m6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment

Background. We analyzed the n6-methyladenosine (m6A) modification patterns of immune cells infiltrating the tumor microenvironment of breast cancer (BC) to provide a new perspective for the early diagnosis and treatment of BC. Methods. Based on 23 m6A regulatory factors, we identified m6A-related ge...

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Autores principales:	Fei Liu, Xiaopeng Yu, Guijin He
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/5ba0801ab1584a1aba77e43ac0c82644
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spelling	oai:doaj.org-article:5ba0801ab1584a1aba77e43ac0c826442021-11-08T02:36:38Zm6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment1687-846910.1155/2021/9987376https://doaj.org/article/5ba0801ab1584a1aba77e43ac0c826442021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9987376https://doaj.org/toc/1687-8469Background. We analyzed the n6-methyladenosine (m6A) modification patterns of immune cells infiltrating the tumor microenvironment of breast cancer (BC) to provide a new perspective for the early diagnosis and treatment of BC. Methods. Based on 23 m6A regulatory factors, we identified m6A-related gene characteristics and m6A modification patterns in BC through unsupervised cluster analysis. To examine the differences in biological processes among various m6A modification modes, we performed genomic variation analysis. We then quantified the relative infiltration levels of different immune cell subpopulations in the tumor microenvironment of BC using the CIBERSORT algorithm and single-sample gene set enrichment analysis. Univariate Cox analysis was used to screen for m6A characteristic genes related to prognosis. Finally, we evaluated the m6A modification pattern of patients with a single BC by constructing the m6Ascore based on principal component analysis. Results. We identified three different m6A modification patterns in 2128 BC samples. A higher abundance of the immune infiltration of the m6Acluster C was indicated by the results of CIBERSORT and the single-sample gene set enrichment analysis. Based on the m6A characteristic genes obtained through screening, the m6Ascore was determined. The BC patients were segregated into m6Ascore groups of low and high categories, which revealed significant survival benefits among patients with low m6Ascores. Additionally, the high-m6Ascore group had a higher mutation frequency and was associated with low PD-L1 expression, and the m6Ascore and tumor mutation burden showed a positive correlation. In addition, treatment effects were better in patients in the high-m6Ascore group. Conclusions. In case of a single patient with BC, the immune cell infiltration characteristics of the tumor microenvironment and the m6A methylation modification pattern could be evaluated using the m6Ascore. Our results provide a foundation for improving personalized immunotherapy of BC.Fei LiuXiaopeng YuGuijin HeHindawi LimitedarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENJournal of Oncology, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Fei Liu Xiaopeng Yu Guijin He m6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment
description	Background. We analyzed the n6-methyladenosine (m6A) modification patterns of immune cells infiltrating the tumor microenvironment of breast cancer (BC) to provide a new perspective for the early diagnosis and treatment of BC. Methods. Based on 23 m6A regulatory factors, we identified m6A-related gene characteristics and m6A modification patterns in BC through unsupervised cluster analysis. To examine the differences in biological processes among various m6A modification modes, we performed genomic variation analysis. We then quantified the relative infiltration levels of different immune cell subpopulations in the tumor microenvironment of BC using the CIBERSORT algorithm and single-sample gene set enrichment analysis. Univariate Cox analysis was used to screen for m6A characteristic genes related to prognosis. Finally, we evaluated the m6A modification pattern of patients with a single BC by constructing the m6Ascore based on principal component analysis. Results. We identified three different m6A modification patterns in 2128 BC samples. A higher abundance of the immune infiltration of the m6Acluster C was indicated by the results of CIBERSORT and the single-sample gene set enrichment analysis. Based on the m6A characteristic genes obtained through screening, the m6Ascore was determined. The BC patients were segregated into m6Ascore groups of low and high categories, which revealed significant survival benefits among patients with low m6Ascores. Additionally, the high-m6Ascore group had a higher mutation frequency and was associated with low PD-L1 expression, and the m6Ascore and tumor mutation burden showed a positive correlation. In addition, treatment effects were better in patients in the high-m6Ascore group. Conclusions. In case of a single patient with BC, the immune cell infiltration characteristics of the tumor microenvironment and the m6A methylation modification pattern could be evaluated using the m6Ascore. Our results provide a foundation for improving personalized immunotherapy of BC.
format	article
author	Fei Liu Xiaopeng Yu Guijin He
author_facet	Fei Liu Xiaopeng Yu Guijin He
author_sort	Fei Liu
title	m6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment
title_short	m6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment
title_full	m6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment
title_fullStr	m6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment
title_full_unstemmed	m6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment
title_sort	m6a-mediated tumor invasion and methylation modification in breast cancer microenvironment
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/5ba0801ab1584a1aba77e43ac0c82644
work_keys_str_mv	AT feiliu m6amediatedtumorinvasionandmethylationmodificationinbreastcancermicroenvironment AT xiaopengyu m6amediatedtumorinvasionandmethylationmodificationinbreastcancermicroenvironment AT guijinhe m6amediatedtumorinvasionandmethylationmodificationinbreastcancermicroenvironment
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m6A-Mediated Tumor Invasion and Methylation Modification in Breast Cancer Microenvironment

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