Accurate localization microscopy by intrinsic aberration calibration
The authors demonstrate accurate localization in three dimensions by comprehensive calibration of an ordinary microscope, exploiting the latent information of intrinsic aberrations. Rigid transformation of the emitter positions tests the method and enables measurements in six degrees of freedom.
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Nature Portfolio
2021
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oai:doaj.org-article:5bb5b08176f84ffc82cdc3a3f46482922021-12-02T17:44:52ZAccurate localization microscopy by intrinsic aberration calibration10.1038/s41467-021-23419-y2041-1723https://doaj.org/article/5bb5b08176f84ffc82cdc3a3f46482922021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23419-yhttps://doaj.org/toc/2041-1723The authors demonstrate accurate localization in three dimensions by comprehensive calibration of an ordinary microscope, exploiting the latent information of intrinsic aberrations. Rigid transformation of the emitter positions tests the method and enables measurements in six degrees of freedom.Craig R. CopelandCraig D. McGrayB. Robert IlicJon GeistSamuel M. StavisNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021) |
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Science Q |
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Science Q Craig R. Copeland Craig D. McGray B. Robert Ilic Jon Geist Samuel M. Stavis Accurate localization microscopy by intrinsic aberration calibration |
description |
The authors demonstrate accurate localization in three dimensions by comprehensive calibration of an ordinary microscope, exploiting the latent information of intrinsic aberrations. Rigid transformation of the emitter positions tests the method and enables measurements in six degrees of freedom. |
format |
article |
author |
Craig R. Copeland Craig D. McGray B. Robert Ilic Jon Geist Samuel M. Stavis |
author_facet |
Craig R. Copeland Craig D. McGray B. Robert Ilic Jon Geist Samuel M. Stavis |
author_sort |
Craig R. Copeland |
title |
Accurate localization microscopy by intrinsic aberration calibration |
title_short |
Accurate localization microscopy by intrinsic aberration calibration |
title_full |
Accurate localization microscopy by intrinsic aberration calibration |
title_fullStr |
Accurate localization microscopy by intrinsic aberration calibration |
title_full_unstemmed |
Accurate localization microscopy by intrinsic aberration calibration |
title_sort |
accurate localization microscopy by intrinsic aberration calibration |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/5bb5b08176f84ffc82cdc3a3f4648292 |
work_keys_str_mv |
AT craigrcopeland accuratelocalizationmicroscopybyintrinsicaberrationcalibration AT craigdmcgray accuratelocalizationmicroscopybyintrinsicaberrationcalibration AT brobertilic accuratelocalizationmicroscopybyintrinsicaberrationcalibration AT jongeist accuratelocalizationmicroscopybyintrinsicaberrationcalibration AT samuelmstavis accuratelocalizationmicroscopybyintrinsicaberrationcalibration |
_version_ |
1718379646048796672 |