Accurate localization microscopy by intrinsic aberration calibration

The authors demonstrate accurate localization in three dimensions by comprehensive calibration of an ordinary microscope, exploiting the latent information of intrinsic aberrations. Rigid transformation of the emitter positions tests the method and enables measurements in six degrees of freedom.

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Autores principales: Craig R. Copeland, Craig D. McGray, B. Robert Ilic, Jon Geist, Samuel M. Stavis
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/5bb5b08176f84ffc82cdc3a3f4648292
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spelling oai:doaj.org-article:5bb5b08176f84ffc82cdc3a3f46482922021-12-02T17:44:52ZAccurate localization microscopy by intrinsic aberration calibration10.1038/s41467-021-23419-y2041-1723https://doaj.org/article/5bb5b08176f84ffc82cdc3a3f46482922021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23419-yhttps://doaj.org/toc/2041-1723The authors demonstrate accurate localization in three dimensions by comprehensive calibration of an ordinary microscope, exploiting the latent information of intrinsic aberrations. Rigid transformation of the emitter positions tests the method and enables measurements in six degrees of freedom.Craig R. CopelandCraig D. McGrayB. Robert IlicJon GeistSamuel M. StavisNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Craig R. Copeland
Craig D. McGray
B. Robert Ilic
Jon Geist
Samuel M. Stavis
Accurate localization microscopy by intrinsic aberration calibration
description The authors demonstrate accurate localization in three dimensions by comprehensive calibration of an ordinary microscope, exploiting the latent information of intrinsic aberrations. Rigid transformation of the emitter positions tests the method and enables measurements in six degrees of freedom.
format article
author Craig R. Copeland
Craig D. McGray
B. Robert Ilic
Jon Geist
Samuel M. Stavis
author_facet Craig R. Copeland
Craig D. McGray
B. Robert Ilic
Jon Geist
Samuel M. Stavis
author_sort Craig R. Copeland
title Accurate localization microscopy by intrinsic aberration calibration
title_short Accurate localization microscopy by intrinsic aberration calibration
title_full Accurate localization microscopy by intrinsic aberration calibration
title_fullStr Accurate localization microscopy by intrinsic aberration calibration
title_full_unstemmed Accurate localization microscopy by intrinsic aberration calibration
title_sort accurate localization microscopy by intrinsic aberration calibration
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/5bb5b08176f84ffc82cdc3a3f4648292
work_keys_str_mv AT craigrcopeland accuratelocalizationmicroscopybyintrinsicaberrationcalibration
AT craigdmcgray accuratelocalizationmicroscopybyintrinsicaberrationcalibration
AT brobertilic accuratelocalizationmicroscopybyintrinsicaberrationcalibration
AT jongeist accuratelocalizationmicroscopybyintrinsicaberrationcalibration
AT samuelmstavis accuratelocalizationmicroscopybyintrinsicaberrationcalibration
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