Design, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer

Yusuf A Haggag,1 Rowida R Ibrahim,2 Amin A Hafiz3 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Tanta University, Tanta, Egypt; 2Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Tanta University, Tanta, Egypt; 3Department of Clinical Nutrition, Faculty of...

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Autores principales:	Haggag YA, Ibrahim RR, Hafiz AA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	honokiol nanocapsule peg-plga copolymers formulation variables anti-cancer activity solid ehrlich carcinoma breast cancer. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5bb6419a61d74e85ab1c8db9eb1b1da0
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spelling	oai:doaj.org-article:5bb6419a61d74e85ab1c8db9eb1b1da02021-12-02T05:48:16ZDesign, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer1178-2013https://doaj.org/article/5bb6419a61d74e85ab1c8db9eb1b1da02020-03-01T00:00:00Zhttps://www.dovepress.com/design-formulation-and-in-vivo-evaluation-of-novel-honokiol-loaded-peg-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yusuf A Haggag,1 Rowida R Ibrahim,2 Amin A Hafiz3 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Tanta University, Tanta, Egypt; 2Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Tanta University, Tanta, Egypt; 3Department of Clinical Nutrition, Faculty of Applied Medical Sciences, Umm Al-Qura University, Mecca, Kingdom of Saudi ArabiaCorrespondence: Yusuf A HaggagDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Tanta University, Tanta 31111, EgyptTel +2 01220104612Fax +2 040 3335466Email youssif.hagag@pharm.tanta.edu.egBackground: Honokiol (HK) is a common herbal medicine extracted from magnolia plants. Low aqueous solubility and limited bioavailability of HK have hindered its clinical application, especially for cancer treatment. Nano-drug delivery system has the potential to enhance HK delivery and therefore, enhance its anti-cancer activity.Purpose: The study’s aim is to design novel PEGylated-PLGA polymeric nanocapsules (NCs) for HK delivery to breast tumor-bearing mice after systemic administration.Methods: Formulation of different HK-loaded NCs and their physio-chemical characterization were optimized through the use of different formulation variables. The antitumor activity of the HK-loaded NCs was investigated both in vitro using MCF-7 and EAC breast cancer cell lines and in vivo using solid Ehrlich carcinoma (SEC) breast cancer model.Results: The optimum HK-loaded NCs were prepared from 15% PEG-PLGA diblock copolymer and exhibited the lowest nano size of 125 nm, smooth spherical morphology, highest drug loading of 94% and highest cellular uptake into breast cancer cells. HK-loaded PEGylated NCs can effectively inhibit the in vitro cell growth of breast cancer cells by 80.2% and 58.1% compared to 35% and 31% with free HK in the case of MCF-7 and EAC, respectively. HK-loaded NCs inhibited SEC tumor growth by 2.3 fold significantly higher than free HK, in vivo.Conclusion: The designed drug delivery system encapsulating HK exhibited a pronounced decrease in tumor growth biomarkers meanwhile proved its safety in animals. Therefore, 15% PEGylated HK-loaded NCs may act as a promising new approach for breast cancer treatment.Keywords: honokiol, nanocapsule, PEG-PLGA copolymers, formulation variables, anti-cancer activity, solid Ehrlich carcinoma, breast cancerHaggag YAIbrahim RRHafiz AADove Medical Pressarticlehonokiolnanocapsulepeg-plga copolymersformulation variablesanti-cancer activitysolid ehrlich carcinomabreast cancer.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 1625-1642 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	honokiol nanocapsule peg-plga copolymers formulation variables anti-cancer activity solid ehrlich carcinoma breast cancer. Medicine (General) R5-920
spellingShingle	honokiol nanocapsule peg-plga copolymers formulation variables anti-cancer activity solid ehrlich carcinoma breast cancer. Medicine (General) R5-920 Haggag YA Ibrahim RR Hafiz AA Design, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer
description	Yusuf A Haggag,1 Rowida R Ibrahim,2 Amin A Hafiz3 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Tanta University, Tanta, Egypt; 2Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Tanta University, Tanta, Egypt; 3Department of Clinical Nutrition, Faculty of Applied Medical Sciences, Umm Al-Qura University, Mecca, Kingdom of Saudi ArabiaCorrespondence: Yusuf A HaggagDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Tanta University, Tanta 31111, EgyptTel +2 01220104612Fax +2 040 3335466Email youssif.hagag@pharm.tanta.edu.egBackground: Honokiol (HK) is a common herbal medicine extracted from magnolia plants. Low aqueous solubility and limited bioavailability of HK have hindered its clinical application, especially for cancer treatment. Nano-drug delivery system has the potential to enhance HK delivery and therefore, enhance its anti-cancer activity.Purpose: The study’s aim is to design novel PEGylated-PLGA polymeric nanocapsules (NCs) for HK delivery to breast tumor-bearing mice after systemic administration.Methods: Formulation of different HK-loaded NCs and their physio-chemical characterization were optimized through the use of different formulation variables. The antitumor activity of the HK-loaded NCs was investigated both in vitro using MCF-7 and EAC breast cancer cell lines and in vivo using solid Ehrlich carcinoma (SEC) breast cancer model.Results: The optimum HK-loaded NCs were prepared from 15% PEG-PLGA diblock copolymer and exhibited the lowest nano size of 125 nm, smooth spherical morphology, highest drug loading of 94% and highest cellular uptake into breast cancer cells. HK-loaded PEGylated NCs can effectively inhibit the in vitro cell growth of breast cancer cells by 80.2% and 58.1% compared to 35% and 31% with free HK in the case of MCF-7 and EAC, respectively. HK-loaded NCs inhibited SEC tumor growth by 2.3 fold significantly higher than free HK, in vivo.Conclusion: The designed drug delivery system encapsulating HK exhibited a pronounced decrease in tumor growth biomarkers meanwhile proved its safety in animals. Therefore, 15% PEGylated HK-loaded NCs may act as a promising new approach for breast cancer treatment.Keywords: honokiol, nanocapsule, PEG-PLGA copolymers, formulation variables, anti-cancer activity, solid Ehrlich carcinoma, breast cancer
format	article
author	Haggag YA Ibrahim RR Hafiz AA
author_facet	Haggag YA Ibrahim RR Hafiz AA
author_sort	Haggag YA
title	Design, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer
title_short	Design, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer
title_full	Design, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer
title_fullStr	Design, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer
title_full_unstemmed	Design, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer
title_sort	design, formulation and in vivo evaluation of novel honokiol-loaded pegylated plga nanocapsules for treatment of breast cancer
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/5bb6419a61d74e85ab1c8db9eb1b1da0
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Design, Formulation and in vivo Evaluation of Novel Honokiol-Loaded PEGylated PLGA Nanocapsules for Treatment of Breast Cancer

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