Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides

Abstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the to...

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Autores principales:	Sushmita Seni, Satinder Kaur, Palvi Malik, Inderjit Singh Yadav, Parul Sirohi, Harsh Chauhan, Amandeep Kaur, Parveen Chhuneja
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5bb79f03f1354edfb29bfdc2db5b788b
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spelling	oai:doaj.org-article:5bb79f03f1354edfb29bfdc2db5b788b2021-11-14T12:21:57ZTranscriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides10.1038/s41598-021-01596-62045-2322https://doaj.org/article/5bb79f03f1354edfb29bfdc2db5b788b2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01596-6https://doaj.org/toc/2045-2322Abstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the total transcriptome profile of heat stress-tolerant Ae. speltoides accession pau3809 (AS3809) and compare with that of tetraploid and hexaploid wheat cultivars PDW274 and PBW725, respectively. The comparative transcriptome was utilized to identify and validate heat stress transcription factors (HSFs), the key genes involved in imparting heat stress tolerance. Transcriptome analysis led to the identification of a total of 74 K, 68 K, and 76 K genes in AS3809, PDW274, and PBW725, respectively. There was a high uniformity of GO profiles under the biological, molecular, and cellular functions across the three wheat transcriptomes, suggesting the conservation of gene function. Twelve HSFs having the highest FPKM value were identified in the AS3809 transcriptome data, while six of these HSFs namely HSFA3, HSFA5, HSFA9, HSFB2a, HSFB2b, and HSFC1b, were validated with qRT PCR. These six HSFs were identified as an important component of thermotolerance in AS3809 as evident from their comparative higher expression under heat stress.Sushmita SeniSatinder KaurPalvi MalikInderjit Singh YadavParul SirohiHarsh ChauhanAmandeep KaurParveen ChhunejaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sushmita Seni Satinder Kaur Palvi Malik Inderjit Singh Yadav Parul Sirohi Harsh Chauhan Amandeep Kaur Parveen Chhuneja Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
description	Abstract Wheat, one of the major cereal crops worldwide, get adversely affected by rising global temperature. We have identified the diploid B genome progenitor of wheat, Aegilops speltoides (SS), as a potential donor for heat stress tolerance. Therefore, the present work was planned to study the total transcriptome profile of heat stress-tolerant Ae. speltoides accession pau3809 (AS3809) and compare with that of tetraploid and hexaploid wheat cultivars PDW274 and PBW725, respectively. The comparative transcriptome was utilized to identify and validate heat stress transcription factors (HSFs), the key genes involved in imparting heat stress tolerance. Transcriptome analysis led to the identification of a total of 74 K, 68 K, and 76 K genes in AS3809, PDW274, and PBW725, respectively. There was a high uniformity of GO profiles under the biological, molecular, and cellular functions across the three wheat transcriptomes, suggesting the conservation of gene function. Twelve HSFs having the highest FPKM value were identified in the AS3809 transcriptome data, while six of these HSFs namely HSFA3, HSFA5, HSFA9, HSFB2a, HSFB2b, and HSFC1b, were validated with qRT PCR. These six HSFs were identified as an important component of thermotolerance in AS3809 as evident from their comparative higher expression under heat stress.
format	article
author	Sushmita Seni Satinder Kaur Palvi Malik Inderjit Singh Yadav Parul Sirohi Harsh Chauhan Amandeep Kaur Parveen Chhuneja
author_facet	Sushmita Seni Satinder Kaur Palvi Malik Inderjit Singh Yadav Parul Sirohi Harsh Chauhan Amandeep Kaur Parveen Chhuneja
author_sort	Sushmita Seni
title	Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_short	Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_full	Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_fullStr	Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_full_unstemmed	Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides
title_sort	transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species aegilops speltoides
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5bb79f03f1354edfb29bfdc2db5b788b
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Transcriptome based identification and validation of heat stress transcription factors in wheat progenitor species Aegilops speltoides

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