Chimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice

YongLi Hou,1 Ting Yan,2 Hui Cao,1 Peng Liu,1 Kang Zheng,1 Zhenyu Li,1 Qing Deng,1 SiHai Hu11Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, People’s Republ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hou Y, Yan T, Cao H, Liu P, Zheng K, Li Z, Deng Q, Hu S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Hepatitis B core protein Neisserial surface protein A virus-like particles recombinant protein vaccine Neisseria meningitidis serogroup B Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/5bb86cdd665344b585ec2e878e100e51
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5bb86cdd665344b585ec2e878e100e51
record_format	dspace
spelling	oai:doaj.org-article:5bb86cdd665344b585ec2e878e100e512021-12-02T09:05:04ZChimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice1178-2013https://doaj.org/article/5bb86cdd665344b585ec2e878e100e512019-08-01T00:00:00Zhttps://www.dovepress.com/chimeric-hepatitis-b-virus-core-particles-displaying-neisserial-surfac-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013YongLi Hou,1 Ting Yan,2 Hui Cao,1 Peng Liu,1 Kang Zheng,1 Zhenyu Li,1 Qing Deng,1 SiHai Hu11Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, People’s Republic of China; 2Department of Health Services, Air Force Medical University, Xi’an, Shaanxi 710032, People’s Republic of ChinaCorrespondence: SiHai HuInstitution of Pathogenic Biology, Hengyang Medical College, University of South China, 28 West Changsheng Road, Hengyang 421001, Hunan, People’s Republic of ChinaTel +86 1 397 340 7567Fax +86 734 828 2907Email hhsshh_518@163.comPurpose: The primary goal of the present study was to explore and evaluate the highly conserved Neisserial surface protein A (NspA) molecule, fused with truncated HBV virus-like particles (VLPs), as a candidate vaccine against the virulent Neisseria meningitidis serogroup B (NMB).Methods: NspA was inserted into the major immunodominant region of the truncated hepatitis B virus core protein (HBc; amino acids 1–144). The chimeric protein, HBc-N144-NspA, was expressed from a prokaryotic vector and generated HBc-like particles, as determined by transmission electron microscopy. Further, the chimeric protein and control proteins were used to immunize mice and the resulting immune responses evaluated by flow cytometry, enzyme-linked immunosorbent assay, and analysis of serum bactericidal activity (SBA) titer.Results: Evaluation of the immunogenicity of the recombinant HBc-N144-NspA protein showed that it elicited the production of high levels of NspA-specific total IgG. The SBA titer of HBc-N144-NspA/F reached 1:16 2 weeks after the last immunization in BALB/c mice, when human serum complement was included in the vaccine. Immunization of HBc-N144-NspA, even without adjuvant, induced high levels of IL-4 and a high IgG1 to IgG2a ratio, confirming induction of an intense Th2 immune response. Levels of IL-17A increased rapidly in mice after the first immunization with HBc-N144-NspA, indicating the potential for this vaccine to induce a mucosal immune response. Meanwhile, the immunization of HBc-N144-NspA without adjuvant induced only mild inflammatory infiltration into the mouse muscle tissue.Conclusion: This study demonstrates that modification using HBc renders NspA a candidate vaccine, which can trigger protective immunity against NMB.Keywords: hepatitis B core protein, Neisserial surface protein A, virus-like particles, recombinant protein vaccine, Neisseria meningitidis serogroup BHou YYan TCao HLiu PZheng KLi ZDeng QHu SDove Medical PressarticleHepatitis B core proteinNeisserial surface protein Avirus-like particlesrecombinant protein vaccineNeisseria meningitidis serogroup BMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 6601-6613 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hepatitis B core protein Neisserial surface protein A virus-like particles recombinant protein vaccine Neisseria meningitidis serogroup B Medicine (General) R5-920
spellingShingle	Hepatitis B core protein Neisserial surface protein A virus-like particles recombinant protein vaccine Neisseria meningitidis serogroup B Medicine (General) R5-920 Hou Y Yan T Cao H Liu P Zheng K Li Z Deng Q Hu S Chimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice
description	YongLi Hou,1 Ting Yan,2 Hui Cao,1 Peng Liu,1 Kang Zheng,1 Zhenyu Li,1 Qing Deng,1 SiHai Hu11Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang 421001, People’s Republic of China; 2Department of Health Services, Air Force Medical University, Xi’an, Shaanxi 710032, People’s Republic of ChinaCorrespondence: SiHai HuInstitution of Pathogenic Biology, Hengyang Medical College, University of South China, 28 West Changsheng Road, Hengyang 421001, Hunan, People’s Republic of ChinaTel +86 1 397 340 7567Fax +86 734 828 2907Email hhsshh_518@163.comPurpose: The primary goal of the present study was to explore and evaluate the highly conserved Neisserial surface protein A (NspA) molecule, fused with truncated HBV virus-like particles (VLPs), as a candidate vaccine against the virulent Neisseria meningitidis serogroup B (NMB).Methods: NspA was inserted into the major immunodominant region of the truncated hepatitis B virus core protein (HBc; amino acids 1–144). The chimeric protein, HBc-N144-NspA, was expressed from a prokaryotic vector and generated HBc-like particles, as determined by transmission electron microscopy. Further, the chimeric protein and control proteins were used to immunize mice and the resulting immune responses evaluated by flow cytometry, enzyme-linked immunosorbent assay, and analysis of serum bactericidal activity (SBA) titer.Results: Evaluation of the immunogenicity of the recombinant HBc-N144-NspA protein showed that it elicited the production of high levels of NspA-specific total IgG. The SBA titer of HBc-N144-NspA/F reached 1:16 2 weeks after the last immunization in BALB/c mice, when human serum complement was included in the vaccine. Immunization of HBc-N144-NspA, even without adjuvant, induced high levels of IL-4 and a high IgG1 to IgG2a ratio, confirming induction of an intense Th2 immune response. Levels of IL-17A increased rapidly in mice after the first immunization with HBc-N144-NspA, indicating the potential for this vaccine to induce a mucosal immune response. Meanwhile, the immunization of HBc-N144-NspA without adjuvant induced only mild inflammatory infiltration into the mouse muscle tissue.Conclusion: This study demonstrates that modification using HBc renders NspA a candidate vaccine, which can trigger protective immunity against NMB.Keywords: hepatitis B core protein, Neisserial surface protein A, virus-like particles, recombinant protein vaccine, Neisseria meningitidis serogroup B
format	article
author	Hou Y Yan T Cao H Liu P Zheng K Li Z Deng Q Hu S
author_facet	Hou Y Yan T Cao H Liu P Zheng K Li Z Deng Q Hu S
author_sort	Hou Y
title	Chimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice
title_short	Chimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice
title_full	Chimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice
title_fullStr	Chimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice
title_full_unstemmed	Chimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice
title_sort	chimeric hepatitis b virus core particles displaying neisserial surface protein a confer protection against virulent neisseria meningitidis serogroup b in balb/c mice
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/5bb86cdd665344b585ec2e878e100e51
work_keys_str_mv	AT houy chimerichepatitisbviruscoreparticlesdisplayingneisserialsurfaceproteinaconferprotectionagainstvirulentneisseriameningitidisserogroupbinbalbcmice AT yant chimerichepatitisbviruscoreparticlesdisplayingneisserialsurfaceproteinaconferprotectionagainstvirulentneisseriameningitidisserogroupbinbalbcmice AT caoh chimerichepatitisbviruscoreparticlesdisplayingneisserialsurfaceproteinaconferprotectionagainstvirulentneisseriameningitidisserogroupbinbalbcmice AT liup chimerichepatitisbviruscoreparticlesdisplayingneisserialsurfaceproteinaconferprotectionagainstvirulentneisseriameningitidisserogroupbinbalbcmice AT zhengk chimerichepatitisbviruscoreparticlesdisplayingneisserialsurfaceproteinaconferprotectionagainstvirulentneisseriameningitidisserogroupbinbalbcmice AT liz chimerichepatitisbviruscoreparticlesdisplayingneisserialsurfaceproteinaconferprotectionagainstvirulentneisseriameningitidisserogroupbinbalbcmice AT dengq chimerichepatitisbviruscoreparticlesdisplayingneisserialsurfaceproteinaconferprotectionagainstvirulentneisseriameningitidisserogroupbinbalbcmice AT hus chimerichepatitisbviruscoreparticlesdisplayingneisserialsurfaceproteinaconferprotectionagainstvirulentneisseriameningitidisserogroupbinbalbcmice
_version_	1718398246332661760

Chimeric hepatitis B virus core particles displaying Neisserial surface protein A confer protection against virulent Neisseria meningitidis serogroup B in BALB/c mice

Ejemplares similares