A functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula

Abstract Aphids are virus-spreading insect pests affecting crops worldwide and their fast population build-up and insecticide resistance make them problematic to control. Here, we aim to understand the molecular basis of spotted alfalfa aphid (SAA) or Therioaphis trifolii f. maculata resistance in M...

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Autores principales:	Silke Jacques, Jana Sperschneider, Gagan Garg, Louise F. Thatcher, Ling-Ling Gao, Lars G. Kamphuis, Karam B. Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5bc3287d8bdf40089837c1f63b64d901
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spelling	oai:doaj.org-article:5bc3287d8bdf40089837c1f63b64d9012021-12-02T12:42:27ZA functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula10.1038/s41598-020-78904-z2045-2322https://doaj.org/article/5bc3287d8bdf40089837c1f63b64d9012020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78904-zhttps://doaj.org/toc/2045-2322Abstract Aphids are virus-spreading insect pests affecting crops worldwide and their fast population build-up and insecticide resistance make them problematic to control. Here, we aim to understand the molecular basis of spotted alfalfa aphid (SAA) or Therioaphis trifolii f. maculata resistance in Medicago truncatula, a model organism for legume species. We compared susceptible and resistant near isogenic Medicago lines upon SAA feeding via transcriptome sequencing. Expression of genes involved in defense and stress responses, protein kinase activity and DNA binding were enriched in the resistant line. Potentially underlying some of these changes in gene expression was the finding that members of the MYB, NAC, AP2 domain and ERF transcription factor gene families were differentially expressed in the resistant versus susceptible lines. A TILLING population created in the resistant cultivar was screened using exome capture sequencing and served as a reverse genetics tool to functionally characterise genes involved in the aphid resistance response. This screening revealed three transcription factors (a NAC, AP2 domain and ERF) as important regulators in the defence response, as a premature stop-codon in the resistant background led to a delay in aphid mortality and enhanced plant susceptibility. This combined functional genomics approach will facilitate the future development of pest resistant crops by uncovering candidate target genes that can convey enhanced aphid resistance.Silke JacquesJana SperschneiderGagan GargLouise F. ThatcherLing-Ling GaoLars G. KamphuisKaram B. SinghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-18 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silke Jacques Jana Sperschneider Gagan Garg Louise F. Thatcher Ling-Ling Gao Lars G. Kamphuis Karam B. Singh A functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula
description	Abstract Aphids are virus-spreading insect pests affecting crops worldwide and their fast population build-up and insecticide resistance make them problematic to control. Here, we aim to understand the molecular basis of spotted alfalfa aphid (SAA) or Therioaphis trifolii f. maculata resistance in Medicago truncatula, a model organism for legume species. We compared susceptible and resistant near isogenic Medicago lines upon SAA feeding via transcriptome sequencing. Expression of genes involved in defense and stress responses, protein kinase activity and DNA binding were enriched in the resistant line. Potentially underlying some of these changes in gene expression was the finding that members of the MYB, NAC, AP2 domain and ERF transcription factor gene families were differentially expressed in the resistant versus susceptible lines. A TILLING population created in the resistant cultivar was screened using exome capture sequencing and served as a reverse genetics tool to functionally characterise genes involved in the aphid resistance response. This screening revealed three transcription factors (a NAC, AP2 domain and ERF) as important regulators in the defence response, as a premature stop-codon in the resistant background led to a delay in aphid mortality and enhanced plant susceptibility. This combined functional genomics approach will facilitate the future development of pest resistant crops by uncovering candidate target genes that can convey enhanced aphid resistance.
format	article
author	Silke Jacques Jana Sperschneider Gagan Garg Louise F. Thatcher Ling-Ling Gao Lars G. Kamphuis Karam B. Singh
author_facet	Silke Jacques Jana Sperschneider Gagan Garg Louise F. Thatcher Ling-Ling Gao Lars G. Kamphuis Karam B. Singh
author_sort	Silke Jacques
title	A functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula
title_short	A functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula
title_full	A functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula
title_fullStr	A functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula
title_full_unstemmed	A functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula
title_sort	functional genomics approach to dissect spotted alfalfa aphid resistance in medicago truncatula
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/5bc3287d8bdf40089837c1f63b64d901
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A functional genomics approach to dissect spotted alfalfa aphid resistance in Medicago truncatula

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