Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma

Abstract Sprouts are germinated seeds that are often consumed due to their high nutritional content and health benefits. However, the conditions for germination strongly support the proliferation of present bacteria, including foodborne pathogens. Since sprouts are consumed raw or minimally processe...

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Autores principales:	A. Waskow, D. Butscher, G. Oberbossel, D. Klöti, P. Rudolf von Rohr, A. Büttner-Mainik, D. Drissner, M. Schuppler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5bc51a0d8e4d4783a13865743b9ca6d3
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spelling	oai:doaj.org-article:5bc51a0d8e4d4783a13865743b9ca6d32021-12-02T18:50:55ZLow-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma10.1038/s41598-021-95767-02045-2322https://doaj.org/article/5bc51a0d8e4d4783a13865743b9ca6d32021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95767-0https://doaj.org/toc/2045-2322Abstract Sprouts are germinated seeds that are often consumed due to their high nutritional content and health benefits. However, the conditions for germination strongly support the proliferation of present bacteria, including foodborne pathogens. Since sprouts are consumed raw or minimally processed, they are frequently linked to cases of food poisoning. Therefore, a seed decontamination method that provides efficient inactivation of microbial pathogens, while maintaining the germination capacity and quality of the seeds is in high demand. This study aimed to investigate and compare seed decontamination by cold atmospheric-pressure plasma and low-energy electron beam with respect to their impact on seed and seedling quality. The results show that both technologies provide great potential for inactivation of microorganisms on seeds, while cold plasma yielded a higher efficiency with 5 log units compared to a maximum of 3 log units after electron beam treatment. Both techniques accelerated seed germination, defined by the percentage of hypocotyl and leaf emergence at 3 days, with short plasma treatment (< 120 s) and all applied doses of electron beam treatment (8–60 kGy). However, even the lowest dose of electron beam treatment at 8 kGy in this study caused root abnormalities in seedlings, suggesting a detrimental effect on the seed tissue. Seeds treated with cold plasma had an eroded seed coat and increased seed wettability compared to electron beam treated seeds. However, these effects cannot explain the increase in the germination capacity of seeds as this was observed for both techniques. Future studies should focus on the investigation of the mechanisms causing accelerated seed germination and root abnormalities by characterizing the molecular and physiological impact of cold plasma and electron beam on seed tissue.A. WaskowD. ButscherG. OberbosselD. KlötiP. Rudolf von RohrA. Büttner-MainikD. DrissnerM. SchupplerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Waskow D. Butscher G. Oberbossel D. Klöti P. Rudolf von Rohr A. Büttner-Mainik D. Drissner M. Schuppler Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma
description	Abstract Sprouts are germinated seeds that are often consumed due to their high nutritional content and health benefits. However, the conditions for germination strongly support the proliferation of present bacteria, including foodborne pathogens. Since sprouts are consumed raw or minimally processed, they are frequently linked to cases of food poisoning. Therefore, a seed decontamination method that provides efficient inactivation of microbial pathogens, while maintaining the germination capacity and quality of the seeds is in high demand. This study aimed to investigate and compare seed decontamination by cold atmospheric-pressure plasma and low-energy electron beam with respect to their impact on seed and seedling quality. The results show that both technologies provide great potential for inactivation of microorganisms on seeds, while cold plasma yielded a higher efficiency with 5 log units compared to a maximum of 3 log units after electron beam treatment. Both techniques accelerated seed germination, defined by the percentage of hypocotyl and leaf emergence at 3 days, with short plasma treatment (< 120 s) and all applied doses of electron beam treatment (8–60 kGy). However, even the lowest dose of electron beam treatment at 8 kGy in this study caused root abnormalities in seedlings, suggesting a detrimental effect on the seed tissue. Seeds treated with cold plasma had an eroded seed coat and increased seed wettability compared to electron beam treated seeds. However, these effects cannot explain the increase in the germination capacity of seeds as this was observed for both techniques. Future studies should focus on the investigation of the mechanisms causing accelerated seed germination and root abnormalities by characterizing the molecular and physiological impact of cold plasma and electron beam on seed tissue.
format	article
author	A. Waskow D. Butscher G. Oberbossel D. Klöti P. Rudolf von Rohr A. Büttner-Mainik D. Drissner M. Schuppler
author_facet	A. Waskow D. Butscher G. Oberbossel D. Klöti P. Rudolf von Rohr A. Büttner-Mainik D. Drissner M. Schuppler
author_sort	A. Waskow
title	Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma
title_short	Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma
title_full	Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma
title_fullStr	Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma
title_full_unstemmed	Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma
title_sort	low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5bc51a0d8e4d4783a13865743b9ca6d3
work_keys_str_mv	AT awaskow lowenergyelectronbeamhassevereimpactonseedlingdevelopmentcomparedtocoldatmosphericpressureplasma AT dbutscher lowenergyelectronbeamhassevereimpactonseedlingdevelopmentcomparedtocoldatmosphericpressureplasma AT goberbossel lowenergyelectronbeamhassevereimpactonseedlingdevelopmentcomparedtocoldatmosphericpressureplasma AT dkloti lowenergyelectronbeamhassevereimpactonseedlingdevelopmentcomparedtocoldatmosphericpressureplasma AT prudolfvonrohr lowenergyelectronbeamhassevereimpactonseedlingdevelopmentcomparedtocoldatmosphericpressureplasma AT abuttnermainik lowenergyelectronbeamhassevereimpactonseedlingdevelopmentcomparedtocoldatmosphericpressureplasma AT ddrissner lowenergyelectronbeamhassevereimpactonseedlingdevelopmentcomparedtocoldatmosphericpressureplasma AT mschuppler lowenergyelectronbeamhassevereimpactonseedlingdevelopmentcomparedtocoldatmosphericpressureplasma
_version_	1718377448025882624

Low-energy electron beam has severe impact on seedling development compared to cold atmospheric pressure plasma

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