A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings

Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 disease. RT-qPCR has been the primary method of diagnosis; however, the required infrastructure is lacking in many developing countries and the virus has remained a global challenge. More inexpen...

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Autores principales:	Erica Frew, Douglas Roberts, Shelly Barry, Matthew Holden, Amanda Restell Mand, Emily Mitsock, Enqing Tan, Wei Yu, Johan Skog
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5bca0fb6db4f425babdb323db478f1a8
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spelling	oai:doaj.org-article:5bca0fb6db4f425babdb323db478f1a82021-11-28T12:19:29ZA SARS-CoV-2 antigen rapid diagnostic test for resource limited settings10.1038/s41598-021-02128-y2045-2322https://doaj.org/article/5bca0fb6db4f425babdb323db478f1a82021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02128-yhttps://doaj.org/toc/2045-2322Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 disease. RT-qPCR has been the primary method of diagnosis; however, the required infrastructure is lacking in many developing countries and the virus has remained a global challenge. More inexpensive and immediate test methods are required to facilitate local, regional, and national management strategies to re-open world economies. Here we have developed a SARS-CoV-2 antigen test in an inexpensive lateral flow format to generate a chromatographic result identifying the presence of the SARS-CoV-2 antigen, and thus an active infection, within a patient anterior nares swab sample. Our 15-min test requires no equipment or laboratory infrastructure to administer with a limit of detection of 2.0 × 102 TCID50/mL and 87.5% sensitivity, 100% specificity when tested against 40 known positive and 40 known negative patient samples established by a validated RT-qPCR test.Erica FrewDouglas RobertsShelly BarryMatthew HoldenAmanda Restell MandEmily MitsockEnqing TanWei YuJohan SkogNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erica Frew Douglas Roberts Shelly Barry Matthew Holden Amanda Restell Mand Emily Mitsock Enqing Tan Wei Yu Johan Skog A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings
description	Abstract Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19 disease. RT-qPCR has been the primary method of diagnosis; however, the required infrastructure is lacking in many developing countries and the virus has remained a global challenge. More inexpensive and immediate test methods are required to facilitate local, regional, and national management strategies to re-open world economies. Here we have developed a SARS-CoV-2 antigen test in an inexpensive lateral flow format to generate a chromatographic result identifying the presence of the SARS-CoV-2 antigen, and thus an active infection, within a patient anterior nares swab sample. Our 15-min test requires no equipment or laboratory infrastructure to administer with a limit of detection of 2.0 × 102 TCID50/mL and 87.5% sensitivity, 100% specificity when tested against 40 known positive and 40 known negative patient samples established by a validated RT-qPCR test.
format	article
author	Erica Frew Douglas Roberts Shelly Barry Matthew Holden Amanda Restell Mand Emily Mitsock Enqing Tan Wei Yu Johan Skog
author_facet	Erica Frew Douglas Roberts Shelly Barry Matthew Holden Amanda Restell Mand Emily Mitsock Enqing Tan Wei Yu Johan Skog
author_sort	Erica Frew
title	A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings
title_short	A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings
title_full	A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings
title_fullStr	A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings
title_full_unstemmed	A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings
title_sort	sars-cov-2 antigen rapid diagnostic test for resource limited settings
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5bca0fb6db4f425babdb323db478f1a8
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A SARS-CoV-2 antigen rapid diagnostic test for resource limited settings

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