Emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria
In this paper, we investigate the epidemiology of infections-associated <i>Staphylococcus</i><i>aureus</i> (<i>S. aureus</i>) from the Medical Intensive Care Unit (MICU) at University Hospital Center of Constantine (UHCC) in Algeria, with a special emphasis on met...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/5bcf1a30d0744f52b51122a54a1a8b42 |
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| Sumario: | In this paper, we investigate the epidemiology of infections-associated <i>Staphylococcus</i><i>aureus</i> (<i>S. aureus</i>) from the Medical Intensive Care Unit (MICU) at University Hospital Center of Constantine (UHCC) in Algeria, with a special emphasis on methicillin-resistant strains (MRSA) revealed by cefoxitin disks (30 μg), then confirmed by penicillin-binding protein (PBP2a) agglutination and real-time polymerase chain reaction (RT-PCR) targeting <i>mecA</i> and <i>mec</i>C genes. Staphylococcal Cassette Chromosome <i>mec</i> (<i>SCCmec</i> type), staphylococcal protein A (<i>spa</i>-type), multilocus sequence type (MLST), Panton–Valentine Leucocidin (PVL), and toxic shock syndrome toxin-1 (TSST-1) were further investigated in all isolates, and whole genome sequencing was performed for a selected subset of three hospital-acquired MRSA (HA-MRSA) isolates. A measurement of 80% out of the 50 <i>S. aureus</i> isolates were identified as HA-MRSA harbouring the <i>mecA</i> gene, and 72.5% of them were multidrug resistant (MDR). Twelve STs, four different <i>SCCmec</i> cassettes, fourteen <i>spa</i> types, ten isolates Panton–Valentine Leukocidin (PVL)-positive, and three isolates TSST-1 were identified. Interestingly, there was a high prevalence (n = 29; 72.5%) of a worrisome emerging clone: the HA-MRSA ST239/241 <i>SCCme</i>c-III mercury with PVL negative, resistant to β-lactams, aminoglycosides, quinolones, and tetracyclines. Other clones of HA-MRSA isolates were also identified, including PVL-positive ST80 <i>SCCmec</i>-IV/<i>SCCmec</i>-unknown (22.5%), ST34 <i>SCCmec</i>-V with TSST-1 positive (2.5%), and PVL-negative ST72 <i>SCCmec</i>-II (2.5%). Genome analysis enables us to describe the first detection of both PVL-negative HA-MRSA ST239/241 SCCmec-III mercury carrying <i>ccrC</i>, as well as <i>SCCmec</i>-V cassette, which dramatically changes the epidemiology of <i>S. aureus</i> infections in one of the hospitals in eastern Algeria. |
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