Emergence of Methicillin-Resistant Staphylococcus aureus ST239/241 SCCmec-III Mercury in Eastern Algeria

In this paper, we investigate the epidemiology of infections-associated Staphylococcusaureus (S. aureus) from the Medical Intensive Care Unit (MICU) at University Hospital Center of Constantine (UHCC) in Algeria, with a special emphasis on met...

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Autores principales:	Hanane Aouati, Linda Hadjadj, Farida Aouati, Amir Agabou, Mariem Ben Khedher, Hacène Bousseboua, Chafia Bentchouala, Jean-Marc Rolain, Seydina M. Diene
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HA-MRSA MDR <i>SCCmec</i> cassettes <i>SCCmec</i>-III mercury-<i>ccrC</i> ST239/241 <i>SCCmec</i>-V Medicine R
Acceso en línea:	https://doaj.org/article/5bcf1a30d0744f52b51122a54a1a8b42
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spelling	oai:doaj.org-article:5bcf1a30d0744f52b51122a54a1a8b422021-11-25T18:38:46ZEmergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria10.3390/pathogens101115032076-0817https://doaj.org/article/5bcf1a30d0744f52b51122a54a1a8b422021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1503https://doaj.org/toc/2076-0817In this paper, we investigate the epidemiology of infections-associated <i>Staphylococcus</i><i>aureus</i> (<i>S. aureus</i>) from the Medical Intensive Care Unit (MICU) at University Hospital Center of Constantine (UHCC) in Algeria, with a special emphasis on methicillin-resistant strains (MRSA) revealed by cefoxitin disks (30 μg), then confirmed by penicillin-binding protein (PBP2a) agglutination and real-time polymerase chain reaction (RT-PCR) targeting <i>mecA</i> and <i>mec</i>C genes. Staphylococcal Cassette Chromosome <i>mec</i> (<i>SCCmec</i> type), staphylococcal protein A (<i>spa</i>-type), multilocus sequence type (MLST), Panton–Valentine Leucocidin (PVL), and toxic shock syndrome toxin-1 (TSST-1) were further investigated in all isolates, and whole genome sequencing was performed for a selected subset of three hospital-acquired MRSA (HA-MRSA) isolates. A measurement of 80% out of the 50 <i>S. aureus</i> isolates were identified as HA-MRSA harbouring the <i>mecA</i> gene, and 72.5% of them were multidrug resistant (MDR). Twelve STs, four different <i>SCCmec</i> cassettes, fourteen <i>spa</i> types, ten isolates Panton–Valentine Leukocidin (PVL)-positive, and three isolates TSST-1 were identified. Interestingly, there was a high prevalence (n = 29; 72.5%) of a worrisome emerging clone: the HA-MRSA ST239/241 <i>SCCme</i>c-III mercury with PVL negative, resistant to β-lactams, aminoglycosides, quinolones, and tetracyclines. Other clones of HA-MRSA isolates were also identified, including PVL-positive ST80 <i>SCCmec</i>-IV/<i>SCCmec</i>-unknown (22.5%), ST34 <i>SCCmec</i>-V with TSST-1 positive (2.5%), and PVL-negative ST72 <i>SCCmec</i>-II (2.5%). Genome analysis enables us to describe the first detection of both PVL-negative HA-MRSA ST239/241 SCCmec-III mercury carrying <i>ccrC</i>, as well as <i>SCCmec</i>-V cassette, which dramatically changes the epidemiology of <i>S. aureus</i> infections in one of the hospitals in eastern Algeria.Hanane AouatiLinda HadjadjFarida AouatiAmir AgabouMariem Ben KhedherHacène BoussebouaChafia BentchoualaJean-Marc RolainSeydina M. DieneMDPI AGarticleHA-MRSAMDR<i>SCCmec</i> cassettes<i>SCCmec</i>-III mercury-<i>ccrC</i>ST239/241<i>SCCmec</i>-VMedicineRENPathogens, Vol 10, Iss 1503, p 1503 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HA-MRSA MDR <i>SCCmec</i> cassettes <i>SCCmec</i>-III mercury-<i>ccrC</i> ST239/241 <i>SCCmec</i>-V Medicine R
spellingShingle	HA-MRSA MDR <i>SCCmec</i> cassettes <i>SCCmec</i>-III mercury-<i>ccrC</i> ST239/241 <i>SCCmec</i>-V Medicine R Hanane Aouati Linda Hadjadj Farida Aouati Amir Agabou Mariem Ben Khedher Hacène Bousseboua Chafia Bentchouala Jean-Marc Rolain Seydina M. Diene Emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria
description	In this paper, we investigate the epidemiology of infections-associated <i>Staphylococcus</i><i>aureus</i> (<i>S. aureus</i>) from the Medical Intensive Care Unit (MICU) at University Hospital Center of Constantine (UHCC) in Algeria, with a special emphasis on methicillin-resistant strains (MRSA) revealed by cefoxitin disks (30 μg), then confirmed by penicillin-binding protein (PBP2a) agglutination and real-time polymerase chain reaction (RT-PCR) targeting <i>mecA</i> and <i>mec</i>C genes. Staphylococcal Cassette Chromosome <i>mec</i> (<i>SCCmec</i> type), staphylococcal protein A (<i>spa</i>-type), multilocus sequence type (MLST), Panton–Valentine Leucocidin (PVL), and toxic shock syndrome toxin-1 (TSST-1) were further investigated in all isolates, and whole genome sequencing was performed for a selected subset of three hospital-acquired MRSA (HA-MRSA) isolates. A measurement of 80% out of the 50 <i>S. aureus</i> isolates were identified as HA-MRSA harbouring the <i>mecA</i> gene, and 72.5% of them were multidrug resistant (MDR). Twelve STs, four different <i>SCCmec</i> cassettes, fourteen <i>spa</i> types, ten isolates Panton–Valentine Leukocidin (PVL)-positive, and three isolates TSST-1 were identified. Interestingly, there was a high prevalence (n = 29; 72.5%) of a worrisome emerging clone: the HA-MRSA ST239/241 <i>SCCme</i>c-III mercury with PVL negative, resistant to β-lactams, aminoglycosides, quinolones, and tetracyclines. Other clones of HA-MRSA isolates were also identified, including PVL-positive ST80 <i>SCCmec</i>-IV/<i>SCCmec</i>-unknown (22.5%), ST34 <i>SCCmec</i>-V with TSST-1 positive (2.5%), and PVL-negative ST72 <i>SCCmec</i>-II (2.5%). Genome analysis enables us to describe the first detection of both PVL-negative HA-MRSA ST239/241 SCCmec-III mercury carrying <i>ccrC</i>, as well as <i>SCCmec</i>-V cassette, which dramatically changes the epidemiology of <i>S. aureus</i> infections in one of the hospitals in eastern Algeria.
format	article
author	Hanane Aouati Linda Hadjadj Farida Aouati Amir Agabou Mariem Ben Khedher Hacène Bousseboua Chafia Bentchouala Jean-Marc Rolain Seydina M. Diene
author_facet	Hanane Aouati Linda Hadjadj Farida Aouati Amir Agabou Mariem Ben Khedher Hacène Bousseboua Chafia Bentchouala Jean-Marc Rolain Seydina M. Diene
author_sort	Hanane Aouati
title	Emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria
title_short	Emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria
title_full	Emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria
title_fullStr	Emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria
title_full_unstemmed	Emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria
title_sort	emergence of methicillin-resistant <i>staphylococcus aureus</i> st239/241 <i>sccmec</i>-iii mercury in eastern algeria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5bcf1a30d0744f52b51122a54a1a8b42
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Emergence of Methicillin-Resistant <i>Staphylococcus aureus</i> ST239/241 <i>SCCmec</i>-III Mercury in Eastern Algeria

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