CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples

CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples

Genomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.

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Autores principales: Xiaolu Zhang, Silvano Garnerone, Michele Simonetti, Luuk Harbers, Marcin Nicoś, Reza Mirzazadeh, Tiziana Venesio, Anna Sapino, Johan Hartman, Caterina Marchiò, Magda Bienko, Nicola Crosetto
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/5bd13737717a4dc7914df1406868bf3e
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spelling oai:doaj.org-article:5bd13737717a4dc7914df1406868bf3e2021-12-02T16:57:42ZCUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples10.1038/s41467-019-12570-22041-1723https://doaj.org/article/5bd13737717a4dc7914df1406868bf3e2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12570-2https://doaj.org/toc/2041-1723Genomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.Xiaolu ZhangSilvano GarneroneMichele SimonettiLuuk HarbersMarcin NicośReza MirzazadehTiziana VenesioAnna SapinoJohan HartmanCaterina MarchiòMagda BienkoNicola CrosettoNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Xiaolu Zhang
Silvano Garnerone
Michele Simonetti
Luuk Harbers
Marcin Nicoś
Reza Mirzazadeh
Tiziana Venesio
Anna Sapino
Johan Hartman
Caterina Marchiò
Magda Bienko
Nicola Crosetto
CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
description Genomics DNA library preparation from formalin-fixed paraffin-embedded tissues is challenging. Here the authors describe CUTseq that uses restriction enzymes and in vitro amplification to barcode samples for reduced representation genome sequencing.
format article
author Xiaolu Zhang
Silvano Garnerone
Michele Simonetti
Luuk Harbers
Marcin Nicoś
Reza Mirzazadeh
Tiziana Venesio
Anna Sapino
Johan Hartman
Caterina Marchiò
Magda Bienko
Nicola Crosetto
author_facet Xiaolu Zhang
Silvano Garnerone
Michele Simonetti
Luuk Harbers
Marcin Nicoś
Reza Mirzazadeh
Tiziana Venesio
Anna Sapino
Johan Hartman
Caterina Marchiò
Magda Bienko
Nicola Crosetto
author_sort Xiaolu Zhang
title CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_short CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_full CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_fullStr CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_full_unstemmed CUTseq is a versatile method for preparing multiplexed DNA sequencing libraries from low-input samples
title_sort cutseq is a versatile method for preparing multiplexed dna sequencing libraries from low-input samples
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/5bd13737717a4dc7914df1406868bf3e
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