Investigation on Mycobacterium tuberculosis diversity in China and the origin of the Beijing clade.

<h4>Background</h4>Investigation of the genetic diversity of Mycobacterium tuberculosis in China has shown that Beijing genotype strains play a dominant role in the tuberculosis (TB) epidemic. In order to examine the strain diversity in the whole country, and to study the evolutionary de...

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Autores principales: Kanglin Wan, Jinghua Liu, Yolande Hauck, Yuanyuan Zhang, Jie Liu, Xiuqin Zhao, Zhiguang Liu, Bing Lu, Haiyan Dong, Yi Jiang, Kristin Kremer, Gilles Vergnaud, Dick van Soolingen, Christine Pourcel
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:5bd5de8bfcbf4faa8f5be6674049438d2021-11-18T07:31:17ZInvestigation on Mycobacterium tuberculosis diversity in China and the origin of the Beijing clade.1932-620310.1371/journal.pone.0029190https://doaj.org/article/5bd5de8bfcbf4faa8f5be6674049438d2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22220207/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Investigation of the genetic diversity of Mycobacterium tuberculosis in China has shown that Beijing genotype strains play a dominant role in the tuberculosis (TB) epidemic. In order to examine the strain diversity in the whole country, and to study the evolutionary development of Beijing strains, we sought to genotype a large collection of isolates using different methods.<h4>Methodology/principal findings</h4>We applied a 15-loci VNTR typing analysis on 1,586 isolates from the Beijing municipality and 12 Chinese provinces or autonomous regions. The data was compared to that of 900 isolates from various other worldwide geographic regions outside of China. A total of 1,162/1,586 (73.2%) of the isolates, distributed into 472 VNTR types, were found to belong to the Beijing genotype family and this represented 56 to 94% of the isolates in each of the localizations. VNTR typing revealed that the majority of the non-Beijing isolates fall into two genotype families, which represented 17% of the total number of isolates, and seem largely restricted to China. A small number of East African Indian genotype strains was also observed in this collection. Ancient Beijing strains with an intact region of difference (RD) 181, as well as strains presumably resembling ancestors of the whole Beijing genotype family, were mainly found in the Guangxi autonomous region.<h4>Conclusions/significance</h4>This is the largest M. tuberculosis VNTR-based genotyping study performed in China to date. The high percentage of Beijing isolates in the whole country and the presence in the South of strains representing early branching points may be an indication that the Beijing lineage originated from China, probably in the Guangxi region. Two modern lineages are shown here to represent the majority of non-Beijing Chinese isolates. The observed geographic distribution of the different lineages within China suggests that natural frontiers are major factors in their diffusion.Kanglin WanJinghua LiuYolande HauckYuanyuan ZhangJie LiuXiuqin ZhaoZhiguang LiuBing LuHaiyan DongYi JiangKristin KremerGilles VergnaudDick van SoolingenChristine PourcelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e29190 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Kanglin Wan
Jinghua Liu
Yolande Hauck
Yuanyuan Zhang
Jie Liu
Xiuqin Zhao
Zhiguang Liu
Bing Lu
Haiyan Dong
Yi Jiang
Kristin Kremer
Gilles Vergnaud
Dick van Soolingen
Christine Pourcel
Investigation on Mycobacterium tuberculosis diversity in China and the origin of the Beijing clade.
description <h4>Background</h4>Investigation of the genetic diversity of Mycobacterium tuberculosis in China has shown that Beijing genotype strains play a dominant role in the tuberculosis (TB) epidemic. In order to examine the strain diversity in the whole country, and to study the evolutionary development of Beijing strains, we sought to genotype a large collection of isolates using different methods.<h4>Methodology/principal findings</h4>We applied a 15-loci VNTR typing analysis on 1,586 isolates from the Beijing municipality and 12 Chinese provinces or autonomous regions. The data was compared to that of 900 isolates from various other worldwide geographic regions outside of China. A total of 1,162/1,586 (73.2%) of the isolates, distributed into 472 VNTR types, were found to belong to the Beijing genotype family and this represented 56 to 94% of the isolates in each of the localizations. VNTR typing revealed that the majority of the non-Beijing isolates fall into two genotype families, which represented 17% of the total number of isolates, and seem largely restricted to China. A small number of East African Indian genotype strains was also observed in this collection. Ancient Beijing strains with an intact region of difference (RD) 181, as well as strains presumably resembling ancestors of the whole Beijing genotype family, were mainly found in the Guangxi autonomous region.<h4>Conclusions/significance</h4>This is the largest M. tuberculosis VNTR-based genotyping study performed in China to date. The high percentage of Beijing isolates in the whole country and the presence in the South of strains representing early branching points may be an indication that the Beijing lineage originated from China, probably in the Guangxi region. Two modern lineages are shown here to represent the majority of non-Beijing Chinese isolates. The observed geographic distribution of the different lineages within China suggests that natural frontiers are major factors in their diffusion.
format article
author Kanglin Wan
Jinghua Liu
Yolande Hauck
Yuanyuan Zhang
Jie Liu
Xiuqin Zhao
Zhiguang Liu
Bing Lu
Haiyan Dong
Yi Jiang
Kristin Kremer
Gilles Vergnaud
Dick van Soolingen
Christine Pourcel
author_facet Kanglin Wan
Jinghua Liu
Yolande Hauck
Yuanyuan Zhang
Jie Liu
Xiuqin Zhao
Zhiguang Liu
Bing Lu
Haiyan Dong
Yi Jiang
Kristin Kremer
Gilles Vergnaud
Dick van Soolingen
Christine Pourcel
author_sort Kanglin Wan
title Investigation on Mycobacterium tuberculosis diversity in China and the origin of the Beijing clade.
title_short Investigation on Mycobacterium tuberculosis diversity in China and the origin of the Beijing clade.
title_full Investigation on Mycobacterium tuberculosis diversity in China and the origin of the Beijing clade.
title_fullStr Investigation on Mycobacterium tuberculosis diversity in China and the origin of the Beijing clade.
title_full_unstemmed Investigation on Mycobacterium tuberculosis diversity in China and the origin of the Beijing clade.
title_sort investigation on mycobacterium tuberculosis diversity in china and the origin of the beijing clade.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/5bd5de8bfcbf4faa8f5be6674049438d
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