Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.

Sperm dimensions and the question of whether X and Y chromosome-bearing sperm differ in size or shape has been of great interest, especially for the development of alternative methods to sort or classify sperm cells. The aim of the present study was to evaluate possible differences in the shape and...

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Autores principales:	José O Carvalho, Luciano P Silva, Roberto Sartori, Margot A N Dode
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5bd6c4a3e33d4f7fa573f1acbea2f032
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spelling	oai:doaj.org-article:5bd6c4a3e33d4f7fa573f1acbea2f0322021-11-18T07:52:51ZNanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.1932-620310.1371/journal.pone.0059387https://doaj.org/article/5bd6c4a3e33d4f7fa573f1acbea2f0322013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23527178/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Sperm dimensions and the question of whether X and Y chromosome-bearing sperm differ in size or shape has been of great interest, especially for the development of alternative methods to sort or classify sperm cells. The aim of the present study was to evaluate possible differences in the shape and size of the sperm head between X and Y chromosome-bearing sperm by atomic force microscopy (AFM). One ejaculate per bull (n = 4) was used. Each ejaculate was separated into four fractions: non-sexed (NS), sexed for X-sperm (SX), sexed for Y-sperm (SY) and a pooling of SX and SY samples (SXY). Using AFM, 400 sperm heads per group were measured. Twenty three structural features were assessed including one-, two- and three-dimensional parameters and shape descriptors. These measurements determine the micro- to nanoscale features of X- and Y-bearing chromosomes in sperm cells. No differences were observed for any individual variables between SX and SY groups. Next, a simultaneous evaluation of all features using statistical discriminant analysis was performed to determine if it was possible to distinguish to which group belong each individual cells. This analysis clearly showed, a distinct separation of NS, SXY, SX and SY groups. The recognition of this structural possibility to distinguish between X and Y sperm cell might improve the understanding of sperm cells biology. These results indicated that the associations of several structural measurements of the sperm cell head are promising candidates for development of a new method of sperm sexing.José O CarvalhoLuciano P SilvaRoberto SartoriMargot A N DodePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59387 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q José O Carvalho Luciano P Silva Roberto Sartori Margot A N Dode Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.
description	Sperm dimensions and the question of whether X and Y chromosome-bearing sperm differ in size or shape has been of great interest, especially for the development of alternative methods to sort or classify sperm cells. The aim of the present study was to evaluate possible differences in the shape and size of the sperm head between X and Y chromosome-bearing sperm by atomic force microscopy (AFM). One ejaculate per bull (n = 4) was used. Each ejaculate was separated into four fractions: non-sexed (NS), sexed for X-sperm (SX), sexed for Y-sperm (SY) and a pooling of SX and SY samples (SXY). Using AFM, 400 sperm heads per group were measured. Twenty three structural features were assessed including one-, two- and three-dimensional parameters and shape descriptors. These measurements determine the micro- to nanoscale features of X- and Y-bearing chromosomes in sperm cells. No differences were observed for any individual variables between SX and SY groups. Next, a simultaneous evaluation of all features using statistical discriminant analysis was performed to determine if it was possible to distinguish to which group belong each individual cells. This analysis clearly showed, a distinct separation of NS, SXY, SX and SY groups. The recognition of this structural possibility to distinguish between X and Y sperm cell might improve the understanding of sperm cells biology. These results indicated that the associations of several structural measurements of the sperm cell head are promising candidates for development of a new method of sperm sexing.
format	article
author	José O Carvalho Luciano P Silva Roberto Sartori Margot A N Dode
author_facet	José O Carvalho Luciano P Silva Roberto Sartori Margot A N Dode
author_sort	José O Carvalho
title	Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.
title_short	Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.
title_full	Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.
title_fullStr	Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.
title_full_unstemmed	Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.
title_sort	nanoscale differences in the shape and size of x and y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5bd6c4a3e33d4f7fa573f1acbea2f032
work_keys_str_mv	AT joseocarvalho nanoscaledifferencesintheshapeandsizeofxandychromosomebearingbovinespermheadsassessedbyatomicforcemicroscopy AT lucianopsilva nanoscaledifferencesintheshapeandsizeofxandychromosomebearingbovinespermheadsassessedbyatomicforcemicroscopy AT robertosartori nanoscaledifferencesintheshapeandsizeofxandychromosomebearingbovinespermheadsassessedbyatomicforcemicroscopy AT margotandode nanoscaledifferencesintheshapeandsizeofxandychromosomebearingbovinespermheadsassessedbyatomicforcemicroscopy
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Nanoscale differences in the shape and size of X and Y chromosome-bearing bovine sperm heads assessed by atomic force microscopy.

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