Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq

Abstract Dendrobium officinale L. is an important traditional herb with high commercial value in China. Several bioactive constituents, including polysaccharides and alkaloids, reportedly make major contributions toward the excellent medicinal effect of D. officinale. In this study, the contents of...

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Main Authors:	Chenjia Shen, Hong Guo, Hailing Chen, Yujun Shi, Yijun Meng, Jiangjie Lu, Shangguo Feng, Huizhong Wang
Format:	article
Language:	EN
Published:	Nature Portfolio 2017
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/5be1ed17d370404c91e7f8dfca0c63d1
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spelling	oai:doaj.org-article:5be1ed17d370404c91e7f8dfca0c63d12021-12-02T16:08:12ZIdentification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq10.1038/s41598-017-00292-82045-2322https://doaj.org/article/5be1ed17d370404c91e7f8dfca0c63d12017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00292-8https://doaj.org/toc/2045-2322Abstract Dendrobium officinale L. is an important traditional herb with high commercial value in China. Several bioactive constituents, including polysaccharides and alkaloids, reportedly make major contributions toward the excellent medicinal effect of D. officinale. In this study, the contents of polysaccharides and alkaloids in various organs of D. officinale were measured and compared. We took advantage of transcriptomes from four organs to explore biological mechanisms in the organ-specific distribution of active ingredients in D. officinale. Based on Kyoto Encyclopedia of Genes and Genomes pathways, unigenes related to the enzymes involved in fructose and mannose metabolism and unigenes associated with putative upstream elements of the alkaloid biosynthetic pathway were identified. A large number of candidates, including 35 full-length glycosyltransferase genes and 49 full-length P450 genes, were also identified based on the transcriptome data, and the organ-specific expression pattern of these genes was determined. Furthermore, differential expression of all candidate genes was analyzed in two Dendrobium species, D. nobile L. and D. officinale. The data will supply important clues to exploit useful genes involved in polysaccharide and alkaloid synthesis.Chenjia ShenHong GuoHailing ChenYujun ShiYijun MengJiangjie LuShangguo FengHuizhong WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chenjia Shen Hong Guo Hailing Chen Yujun Shi Yijun Meng Jiangjie Lu Shangguo Feng Huizhong Wang Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq
description	Abstract Dendrobium officinale L. is an important traditional herb with high commercial value in China. Several bioactive constituents, including polysaccharides and alkaloids, reportedly make major contributions toward the excellent medicinal effect of D. officinale. In this study, the contents of polysaccharides and alkaloids in various organs of D. officinale were measured and compared. We took advantage of transcriptomes from four organs to explore biological mechanisms in the organ-specific distribution of active ingredients in D. officinale. Based on Kyoto Encyclopedia of Genes and Genomes pathways, unigenes related to the enzymes involved in fructose and mannose metabolism and unigenes associated with putative upstream elements of the alkaloid biosynthetic pathway were identified. A large number of candidates, including 35 full-length glycosyltransferase genes and 49 full-length P450 genes, were also identified based on the transcriptome data, and the organ-specific expression pattern of these genes was determined. Furthermore, differential expression of all candidate genes was analyzed in two Dendrobium species, D. nobile L. and D. officinale. The data will supply important clues to exploit useful genes involved in polysaccharide and alkaloid synthesis.
format	article
author	Chenjia Shen Hong Guo Hailing Chen Yujun Shi Yijun Meng Jiangjie Lu Shangguo Feng Huizhong Wang
author_facet	Chenjia Shen Hong Guo Hailing Chen Yujun Shi Yijun Meng Jiangjie Lu Shangguo Feng Huizhong Wang
author_sort	Chenjia Shen
title	Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq
title_short	Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq
title_full	Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq
title_fullStr	Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq
title_full_unstemmed	Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq
title_sort	identification and analysis of genes associated with the synthesis of bioactive constituents in dendrobium officinale using rna-seq
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5be1ed17d370404c91e7f8dfca0c63d1
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Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq

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