High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.

<h4>Background</h4>Antimicrobial peptide expression is associated with disease activity in inflammatory bowel disease (IBD) patients. IBD patients have abnormal expression of elafin, a human elastase-specific protease inhibitor and antimicrobial peptide. We determined elafin expression i...

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Autores principales:	Jiani Wang, Christina Ortiz, Lindsey Fontenot, Ying Xie, Wendy Ho, S Anjani Mattai, David Q Shih, Hon Wai Koon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5bf9df99799348ad8ba254e231ee6dd8
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spelling	oai:doaj.org-article:5bf9df99799348ad8ba254e231ee6dd82021-12-02T20:18:57ZHigh circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.1932-620310.1371/journal.pone.0231796https://doaj.org/article/5bf9df99799348ad8ba254e231ee6dd82020-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0231796https://doaj.org/toc/1932-6203<h4>Background</h4>Antimicrobial peptide expression is associated with disease activity in inflammatory bowel disease (IBD) patients. IBD patients have abnormal expression of elafin, a human elastase-specific protease inhibitor and antimicrobial peptide. We determined elafin expression in blood, intestine, and mesenteric fat of IBD and non-IBD patients.<h4>Methods</h4>Serum samples from normal and IBD patients were collected from two UCLA cohorts. Surgical resection samples of human colonic and mesenteric fat tissues from IBD and non-IBD (colon cancer) patients were collected from Cedars-Sinai Medical Center.<h4>Results</h4>High serum elafin levels were associated with a significantly elevated risk of intestinal stricture in Crohn's disease (CD) patients. Microsoft Azure Machine learning algorithm using serum elafin levels and clinical data identified stricturing CD patients with high accuracy. Serum elafin levels had weak positive correlations with clinical disease activity (Partial Mayo Score and Harvey Bradshaw Index), but not endoscopic disease activity (Mayo Endoscopic Subscore and Simple Endoscopic Index for CD) in IBD patients. Ulcerative colitis (UC) patients had high serum elafin levels. Colonic elafin mRNA and protein expression were not associated with clinical disease activity and histological injury in IBD patients, but stricturing CD patients had lower colonic elafin expression than non-stricturing CD patients. Mesenteric fat in stricturing CD patients had significantly increased elafin mRNA and protein expression, which may contribute to high circulating elafin levels. Human mesenteric fat adipocytes secrete elafin protein.<h4>Conclusions</h4>High circulating elafin levels are associated with the presence of stricture in CD patients. Serum elafin levels may help identify intestinal strictures in CD patients.Jiani WangChristina OrtizLindsey FontenotYing XieWendy HoS Anjani MattaiDavid Q ShihHon Wai KoonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 15, Iss 4, p e0231796 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiani Wang Christina Ortiz Lindsey Fontenot Ying Xie Wendy Ho S Anjani Mattai David Q Shih Hon Wai Koon High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.
description	<h4>Background</h4>Antimicrobial peptide expression is associated with disease activity in inflammatory bowel disease (IBD) patients. IBD patients have abnormal expression of elafin, a human elastase-specific protease inhibitor and antimicrobial peptide. We determined elafin expression in blood, intestine, and mesenteric fat of IBD and non-IBD patients.<h4>Methods</h4>Serum samples from normal and IBD patients were collected from two UCLA cohorts. Surgical resection samples of human colonic and mesenteric fat tissues from IBD and non-IBD (colon cancer) patients were collected from Cedars-Sinai Medical Center.<h4>Results</h4>High serum elafin levels were associated with a significantly elevated risk of intestinal stricture in Crohn's disease (CD) patients. Microsoft Azure Machine learning algorithm using serum elafin levels and clinical data identified stricturing CD patients with high accuracy. Serum elafin levels had weak positive correlations with clinical disease activity (Partial Mayo Score and Harvey Bradshaw Index), but not endoscopic disease activity (Mayo Endoscopic Subscore and Simple Endoscopic Index for CD) in IBD patients. Ulcerative colitis (UC) patients had high serum elafin levels. Colonic elafin mRNA and protein expression were not associated with clinical disease activity and histological injury in IBD patients, but stricturing CD patients had lower colonic elafin expression than non-stricturing CD patients. Mesenteric fat in stricturing CD patients had significantly increased elafin mRNA and protein expression, which may contribute to high circulating elafin levels. Human mesenteric fat adipocytes secrete elafin protein.<h4>Conclusions</h4>High circulating elafin levels are associated with the presence of stricture in CD patients. Serum elafin levels may help identify intestinal strictures in CD patients.
format	article
author	Jiani Wang Christina Ortiz Lindsey Fontenot Ying Xie Wendy Ho S Anjani Mattai David Q Shih Hon Wai Koon
author_facet	Jiani Wang Christina Ortiz Lindsey Fontenot Ying Xie Wendy Ho S Anjani Mattai David Q Shih Hon Wai Koon
author_sort	Jiani Wang
title	High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.
title_short	High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.
title_full	High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.
title_fullStr	High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.
title_full_unstemmed	High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.
title_sort	high circulating elafin levels are associated with crohn's disease-associated intestinal strictures.
publisher	Public Library of Science (PLoS)
publishDate	2020
url	https://doaj.org/article/5bf9df99799348ad8ba254e231ee6dd8
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High circulating elafin levels are associated with Crohn's disease-associated intestinal strictures.

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