Functional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.

<h4>Background</h4>RIG-I (retinoic acid inducible gene-I) is one of the key cytosolic pattern recognition receptors (PRRs) for detecting nucleotide pathogen associated molecular patterns (PAMPs) and mediating the induction of type I interferon and inflammatory cytokines in innate immune...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lijun Chen, Jianguo Su, Chunrong Yang, Limin Peng, Quanyuan Wan, Lan Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c00fffd2d6f4aeb8d1afac12420200d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5c00fffd2d6f4aeb8d1afac12420200d
record_format	dspace
spelling	oai:doaj.org-article:5c00fffd2d6f4aeb8d1afac12420200d2021-11-18T07:10:14ZFunctional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.1932-620310.1371/journal.pone.0042182https://doaj.org/article/5c00fffd2d6f4aeb8d1afac12420200d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22860079/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>RIG-I (retinoic acid inducible gene-I) is one of the key cytosolic pattern recognition receptors (PRRs) for detecting nucleotide pathogen associated molecular patterns (PAMPs) and mediating the induction of type I interferon and inflammatory cytokines in innate immune response. Though the mechanism is well characterized in mammals, the study of the accurate function of RIG-I in teleosts is still in its infancy.<h4>Methodology/principal findings</h4>To clarify the functional characterizations of RIG-I in grass carp Ctenopharyngodon idella (CiRIG-I), six representative overexpression plasmids were constructed and transfected into C. idella kidney (CIK) cell lines to obtain stably expressing recombinant proteins, respectively. A virus titer test and 96-well plate staining assay showed that all constructs exhibited the antiviral activity somewhat. The quantitative real-time RT-PCR (qRT-PCR) demonstrated that mRNA expressions of CiIPS-1, CiIFN-I and CiMx2 were regulated by not only virus (GCRV) or viral PAMP (poly(IC)) challenge but also bacterial PAMPs (LPS and PGN) stimulation in the steadily transfected cells. The results showed that the full-length CiRIG-I played a key role in RLR pathway. The repressor domain (RD) exerted an inhibitory function of the signaling channel under all utilized challenges. Caspase activation and recruitment domains (CARDs) showed a positive role in GCRV and poly(I:C) challenge. Helicase motifs were crucial for the signaling pathway upon LPS and PGN stimulation. Interestingly, ΔCARDs (CARDs deleted) showed positive modulation in RIG-I signal transduction.<h4>Conclusions/significance</h4>The results provided some novel insights into RIG-I sensing with a strikingly broad regulation in teleosts, responding not only to the dsRNA virus or synthetic dsRNA but also bacterial PAMPs.Lijun ChenJianguo SuJianguo SuChunrong YangLimin PengQuanyuan WanLan WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e42182 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lijun Chen Jianguo Su Jianguo Su Chunrong Yang Limin Peng Quanyuan Wan Lan Wang Functional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.
description	<h4>Background</h4>RIG-I (retinoic acid inducible gene-I) is one of the key cytosolic pattern recognition receptors (PRRs) for detecting nucleotide pathogen associated molecular patterns (PAMPs) and mediating the induction of type I interferon and inflammatory cytokines in innate immune response. Though the mechanism is well characterized in mammals, the study of the accurate function of RIG-I in teleosts is still in its infancy.<h4>Methodology/principal findings</h4>To clarify the functional characterizations of RIG-I in grass carp Ctenopharyngodon idella (CiRIG-I), six representative overexpression plasmids were constructed and transfected into C. idella kidney (CIK) cell lines to obtain stably expressing recombinant proteins, respectively. A virus titer test and 96-well plate staining assay showed that all constructs exhibited the antiviral activity somewhat. The quantitative real-time RT-PCR (qRT-PCR) demonstrated that mRNA expressions of CiIPS-1, CiIFN-I and CiMx2 were regulated by not only virus (GCRV) or viral PAMP (poly(IC)) challenge but also bacterial PAMPs (LPS and PGN) stimulation in the steadily transfected cells. The results showed that the full-length CiRIG-I played a key role in RLR pathway. The repressor domain (RD) exerted an inhibitory function of the signaling channel under all utilized challenges. Caspase activation and recruitment domains (CARDs) showed a positive role in GCRV and poly(I:C) challenge. Helicase motifs were crucial for the signaling pathway upon LPS and PGN stimulation. Interestingly, ΔCARDs (CARDs deleted) showed positive modulation in RIG-I signal transduction.<h4>Conclusions/significance</h4>The results provided some novel insights into RIG-I sensing with a strikingly broad regulation in teleosts, responding not only to the dsRNA virus or synthetic dsRNA but also bacterial PAMPs.
format	article
author	Lijun Chen Jianguo Su Jianguo Su Chunrong Yang Limin Peng Quanyuan Wan Lan Wang
author_facet	Lijun Chen Jianguo Su Jianguo Su Chunrong Yang Limin Peng Quanyuan Wan Lan Wang
author_sort	Lijun Chen
title	Functional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.
title_short	Functional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.
title_full	Functional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.
title_fullStr	Functional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.
title_full_unstemmed	Functional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.
title_sort	functional characterizations of rig-i to gcrv and viral/bacterial pamps in grass carp ctenopharyngodon idella.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5c00fffd2d6f4aeb8d1afac12420200d
work_keys_str_mv	AT lijunchen functionalcharacterizationsofrigitogcrvandviralbacterialpampsingrasscarpctenopharyngodonidella AT jianguosu functionalcharacterizationsofrigitogcrvandviralbacterialpampsingrasscarpctenopharyngodonidella AT jianguosu functionalcharacterizationsofrigitogcrvandviralbacterialpampsingrasscarpctenopharyngodonidella AT chunrongyang functionalcharacterizationsofrigitogcrvandviralbacterialpampsingrasscarpctenopharyngodonidella AT liminpeng functionalcharacterizationsofrigitogcrvandviralbacterialpampsingrasscarpctenopharyngodonidella AT quanyuanwan functionalcharacterizationsofrigitogcrvandviralbacterialpampsingrasscarpctenopharyngodonidella AT lanwang functionalcharacterizationsofrigitogcrvandviralbacterialpampsingrasscarpctenopharyngodonidella
_version_	1718423853338722304

Functional characterizations of RIG-I to GCRV and viral/bacterial PAMPs in grass carp Ctenopharyngodon idella.

Ejemplares similares