Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model

David C Markel1, S Trent Guthrie2, Bin Wu3, Zheng Song4, Paul H Wooley41Department of Orthopaedics, Providence Hospital and Medical Centers, Southfield, MI, 2Henry Ford Hospital, Detroit, MI, 3Department of Biomedical Engineering, Wayne State University, Detroit, MI, 4Orthopaedic Research Institute,...

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Autores principales:	Markel DC, Guthrie ST, Wu B, Song Z, Wooley PH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/5c076b2ec5f04e82a7d13361cc160b98
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spelling	oai:doaj.org-article:5c076b2ec5f04e82a7d13361cc160b982021-12-02T05:54:28ZCharacterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model1178-7031https://doaj.org/article/5c076b2ec5f04e82a7d13361cc160b982012-01-01T00:00:00Zhttp://www.dovepress.com/characterization-of-the-inflammatory-response-to-four-commercial-bone--a9077https://doaj.org/toc/1178-7031David C Markel1, S Trent Guthrie2, Bin Wu3, Zheng Song4, Paul H Wooley41Department of Orthopaedics, Providence Hospital and Medical Centers, Southfield, MI, 2Henry Ford Hospital, Detroit, MI, 3Department of Biomedical Engineering, Wayne State University, Detroit, MI, 4Orthopaedic Research Institute, Wichita, KS, USAAbstract: Bone grafting is utilized in nearly all orthopedic subspecialties and in most anatomic regions. Bone graft substitutes have the potential to offer similar efficacy as autogenous grafts without the morbidity of harvest. Several studies have noted the efficacy of new-generation bone substitute products, but few studies have evaluated their safety. This study characterizes and quantifies the inflammatory reaction to four different commercially available bone graft substitutes, which were examined using the in vivo murine air pouch biocompatibility model. One coralline hydroxyapatite product was chosen as an example of a purely osteoconductive material. Three demineralized bone matrix products were chosen to represent products that are both osteoconductive and osteoinductive. Samples were implanted in a murine air pouch and harvested after 14 days in situ. Pouch fluid was extracted, mRNA isolated, and reverse transcription polymerase chain reactions carried out to detect interleukin-1 gene expression as a marker for inflammation. In addition, multiple histological characteristics were examined to quantify cellular responses to the implanted materials. All bone graft substitutes induced a significant inflammatory response compared with negative controls. Histology and polymerase chain reaction data indicated that the level of inflammatory reaction was elevated in materials with a higher demineralized bone matrix to carrier proportion. The hydroxyapatite product generated a low inflammatory reaction. In conclusion, this study used an in vivo model of biocompatibility to demonstrate that a significant inflammatory reaction occurs when using implanted bone graft substitutes. When choosing a bone grafting method, surgeons should consider both the efficacy and safety of methods and materials used. Further studies are necessary to determine the ideal bone graft material to maximize efficacy while minimizing morbidity.Keywords: bone graft, biocompatibility, inflammation, animal modelMarkel DCGuthrie STWu BSong ZWooley PHDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2012, Iss default, Pp 13-18 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Markel DC Guthrie ST Wu B Song Z Wooley PH Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model
description	David C Markel1, S Trent Guthrie2, Bin Wu3, Zheng Song4, Paul H Wooley41Department of Orthopaedics, Providence Hospital and Medical Centers, Southfield, MI, 2Henry Ford Hospital, Detroit, MI, 3Department of Biomedical Engineering, Wayne State University, Detroit, MI, 4Orthopaedic Research Institute, Wichita, KS, USAAbstract: Bone grafting is utilized in nearly all orthopedic subspecialties and in most anatomic regions. Bone graft substitutes have the potential to offer similar efficacy as autogenous grafts without the morbidity of harvest. Several studies have noted the efficacy of new-generation bone substitute products, but few studies have evaluated their safety. This study characterizes and quantifies the inflammatory reaction to four different commercially available bone graft substitutes, which were examined using the in vivo murine air pouch biocompatibility model. One coralline hydroxyapatite product was chosen as an example of a purely osteoconductive material. Three demineralized bone matrix products were chosen to represent products that are both osteoconductive and osteoinductive. Samples were implanted in a murine air pouch and harvested after 14 days in situ. Pouch fluid was extracted, mRNA isolated, and reverse transcription polymerase chain reactions carried out to detect interleukin-1 gene expression as a marker for inflammation. In addition, multiple histological characteristics were examined to quantify cellular responses to the implanted materials. All bone graft substitutes induced a significant inflammatory response compared with negative controls. Histology and polymerase chain reaction data indicated that the level of inflammatory reaction was elevated in materials with a higher demineralized bone matrix to carrier proportion. The hydroxyapatite product generated a low inflammatory reaction. In conclusion, this study used an in vivo model of biocompatibility to demonstrate that a significant inflammatory reaction occurs when using implanted bone graft substitutes. When choosing a bone grafting method, surgeons should consider both the efficacy and safety of methods and materials used. Further studies are necessary to determine the ideal bone graft material to maximize efficacy while minimizing morbidity.Keywords: bone graft, biocompatibility, inflammation, animal model
format	article
author	Markel DC Guthrie ST Wu B Song Z Wooley PH
author_facet	Markel DC Guthrie ST Wu B Song Z Wooley PH
author_sort	Markel DC
title	Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model
title_short	Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model
title_full	Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model
title_fullStr	Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model
title_full_unstemmed	Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model
title_sort	characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/5c076b2ec5f04e82a7d13361cc160b98
work_keys_str_mv	AT markeldc characterizationoftheinflammatoryresponsetofourcommercialbonegraftsubstitutesusingamurinebiocompatibilitymodel AT guthriest characterizationoftheinflammatoryresponsetofourcommercialbonegraftsubstitutesusingamurinebiocompatibilitymodel AT wub characterizationoftheinflammatoryresponsetofourcommercialbonegraftsubstitutesusingamurinebiocompatibilitymodel AT songz characterizationoftheinflammatoryresponsetofourcommercialbonegraftsubstitutesusingamurinebiocompatibilitymodel AT wooleyph characterizationoftheinflammatoryresponsetofourcommercialbonegraftsubstitutesusingamurinebiocompatibilitymodel
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Characterization of the inflammatory response to four commercial bone graft substitutes using a murine biocompatibility model

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