Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.

In this study, nine C. muris and 43 C. andersoni isolates from various animals in China were subtyped by a multilocus sequence typing (MLST) tool. DNA sequence analyses showed the presence of 1-2 subtypes of C. muris and 2-6 subtypes of C. andersoni at each of the four loci (MS1, MS2, MS3, and MS16)...

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Autores principales:	Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen Ning, Aiqin Liu, Jinfeng Zhao, Yaoyu Feng, Meng Qi, Helei Wang, Chaochao Lv, Guanghui Zhao, Lihua Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c09f108e556406abbaf52513fe8cba5
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spelling	oai:doaj.org-article:5c09f108e556406abbaf52513fe8cba52021-11-18T07:07:41ZMultilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.1932-620310.1371/journal.pone.0043782https://doaj.org/article/5c09f108e556406abbaf52513fe8cba52012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22937094/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In this study, nine C. muris and 43 C. andersoni isolates from various animals in China were subtyped by a multilocus sequence typing (MLST) tool. DNA sequence analyses showed the presence of 1-2 subtypes of C. muris and 2-6 subtypes of C. andersoni at each of the four loci (MS1, MS2, MS3, and MS16), nine of which represented new subtypes. Altogether, two C. muris and 10 C. andersoni MLST subtypes were detected. Linkage disequilibrium analysis indicated although the overall population structure of the two parasites was clonal, the Chinese C. andersoni in cattle has an epidemic structure. Three and two clusters were produced in the C. muris and C. andersoni populations by Structure 2.3.3 analysis, with Chinese C. muris and C. andersoni substructures differing from other countries. Thus, this study suggested the prevalence of C. andersoni in China is not attributed to the introduction of dairy cattle. More studies involving more genetic loci and systematic sampling are needed to better elucidate the population genetic structure of C. muris and C. andersoni in the world and the genetic basis for the difference in host specificity among the two most common gastric parasites.Rongjun WangFuchun JianLongxian ZhangChangshen NingAiqin LiuJinfeng ZhaoYaoyu FengMeng QiHelei WangChaochao LvGuanghui ZhaoLihua XiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43782 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rongjun Wang Fuchun Jian Longxian Zhang Changshen Ning Aiqin Liu Jinfeng Zhao Yaoyu Feng Meng Qi Helei Wang Chaochao Lv Guanghui Zhao Lihua Xiao Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.
description	In this study, nine C. muris and 43 C. andersoni isolates from various animals in China were subtyped by a multilocus sequence typing (MLST) tool. DNA sequence analyses showed the presence of 1-2 subtypes of C. muris and 2-6 subtypes of C. andersoni at each of the four loci (MS1, MS2, MS3, and MS16), nine of which represented new subtypes. Altogether, two C. muris and 10 C. andersoni MLST subtypes were detected. Linkage disequilibrium analysis indicated although the overall population structure of the two parasites was clonal, the Chinese C. andersoni in cattle has an epidemic structure. Three and two clusters were produced in the C. muris and C. andersoni populations by Structure 2.3.3 analysis, with Chinese C. muris and C. andersoni substructures differing from other countries. Thus, this study suggested the prevalence of C. andersoni in China is not attributed to the introduction of dairy cattle. More studies involving more genetic loci and systematic sampling are needed to better elucidate the population genetic structure of C. muris and C. andersoni in the world and the genetic basis for the difference in host specificity among the two most common gastric parasites.
format	article
author	Rongjun Wang Fuchun Jian Longxian Zhang Changshen Ning Aiqin Liu Jinfeng Zhao Yaoyu Feng Meng Qi Helei Wang Chaochao Lv Guanghui Zhao Lihua Xiao
author_facet	Rongjun Wang Fuchun Jian Longxian Zhang Changshen Ning Aiqin Liu Jinfeng Zhao Yaoyu Feng Meng Qi Helei Wang Chaochao Lv Guanghui Zhao Lihua Xiao
author_sort	Rongjun Wang
title	Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.
title_short	Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.
title_full	Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.
title_fullStr	Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.
title_full_unstemmed	Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.
title_sort	multilocus sequence subtyping and genetic structure of cryptosporidium muris and cryptosporidium andersoni.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/5c09f108e556406abbaf52513fe8cba5
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Multilocus sequence subtyping and genetic structure of Cryptosporidium muris and Cryptosporidium andersoni.

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