Genetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.

Phlebotomus argentipes is the vector of Leishmania donovani which causes the disease leishmaniasis, a neglected tropical disease and a growing health problem in Sri Lanka. A proper understanding of the population genetic structure of sand fly vectors is considered important prior to planning and imp...

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Autores principales:	Dulani Ruwanika K Pathirage, Thilini C Weeraratne, Sanath C Senanayake, S H P Parakrama Karunaratne, Nadira D Karunaweera
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5c0d096b327d42358aaf17784b159f9e
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spelling	oai:doaj.org-article:5c0d096b327d42358aaf17784b159f9e2021-12-02T20:14:35ZGenetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.1932-620310.1371/journal.pone.0256819https://doaj.org/article/5c0d096b327d42358aaf17784b159f9e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256819https://doaj.org/toc/1932-6203Phlebotomus argentipes is the vector of Leishmania donovani which causes the disease leishmaniasis, a neglected tropical disease and a growing health problem in Sri Lanka. A proper understanding of the population genetic structure of sand fly vectors is considered important prior to planning and implementation of a successful vector control program. Thus, the present study was conducted to determine the population genetic structure of sand fly vectors in Sri Lanka. Two mitochondrial genes namely Cytochrome c oxidase subunit 1 (Cox 1) and Cytochrome b (Cytb), and the internal transcribed spacer 2 (ITS2) region from the nuclear ribosomal DNA were used for molecular characterization. Analyses included maximum likelihood method, network analysis and DNA polymorphisms. The outcome revealed unique sequences of all genomic regions studied except the cox 1 gene had a relationship with sand flies isolated previously from Sri Lanka, India and Israel and cytb gene of 4 sand flies that aligned with those isolated earlier from Sri Lanka and 3 from Madagascar. Furthermore, cox 1 gene and ITS 2 region analyses based on FST values indicated a possible gene flow between the study sites whereas cytb gene analysis favoured the existence of genetically distinct populations of P. argentipes in each of the study sites. Poor population differentiation of P. argentipes, a possible consequence of a gene flow, is indeed of concern due to the risk imposed by promoting the spread of functionally important phenotypes such as insecticide resistance across the country, making future vector control efforts challenging.Dulani Ruwanika K PathirageThilini C WeeraratneSanath C SenanayakeS H P Parakrama KarunaratneNadira D KarunaweeraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256819 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dulani Ruwanika K Pathirage Thilini C Weeraratne Sanath C Senanayake S H P Parakrama Karunaratne Nadira D Karunaweera Genetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.
description	Phlebotomus argentipes is the vector of Leishmania donovani which causes the disease leishmaniasis, a neglected tropical disease and a growing health problem in Sri Lanka. A proper understanding of the population genetic structure of sand fly vectors is considered important prior to planning and implementation of a successful vector control program. Thus, the present study was conducted to determine the population genetic structure of sand fly vectors in Sri Lanka. Two mitochondrial genes namely Cytochrome c oxidase subunit 1 (Cox 1) and Cytochrome b (Cytb), and the internal transcribed spacer 2 (ITS2) region from the nuclear ribosomal DNA were used for molecular characterization. Analyses included maximum likelihood method, network analysis and DNA polymorphisms. The outcome revealed unique sequences of all genomic regions studied except the cox 1 gene had a relationship with sand flies isolated previously from Sri Lanka, India and Israel and cytb gene of 4 sand flies that aligned with those isolated earlier from Sri Lanka and 3 from Madagascar. Furthermore, cox 1 gene and ITS 2 region analyses based on FST values indicated a possible gene flow between the study sites whereas cytb gene analysis favoured the existence of genetically distinct populations of P. argentipes in each of the study sites. Poor population differentiation of P. argentipes, a possible consequence of a gene flow, is indeed of concern due to the risk imposed by promoting the spread of functionally important phenotypes such as insecticide resistance across the country, making future vector control efforts challenging.
format	article
author	Dulani Ruwanika K Pathirage Thilini C Weeraratne Sanath C Senanayake S H P Parakrama Karunaratne Nadira D Karunaweera
author_facet	Dulani Ruwanika K Pathirage Thilini C Weeraratne Sanath C Senanayake S H P Parakrama Karunaratne Nadira D Karunaweera
author_sort	Dulani Ruwanika K Pathirage
title	Genetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.
title_short	Genetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.
title_full	Genetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.
title_fullStr	Genetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.
title_full_unstemmed	Genetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.
title_sort	genetic diversity and population structure of phlebotomus argentipes: vector of leishmania donovani in sri lanka.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5c0d096b327d42358aaf17784b159f9e
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Genetic diversity and population structure of Phlebotomus argentipes: Vector of Leishmania donovani in Sri Lanka.

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